Background-free imaging of single membrane-receptors with nanophosphors

使用纳米磷光体对单膜受体进行无背景成像

• 批准号: DP170104749
• 负责人: A/Prof Andrei Zvyagin
• 金额: $ 24.91万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170104749
• 关键词: Background; free; imaging; single; membrane

This project aims to develop nanophosphor beacons and real-time, ultrahigh-sensitivity functional imaging to provide a picture of the brain. Time-gated detection microscopy will give these nanophosphors a superior optical contrast. The nanophosphors’ antibody-targeting will image single AMPA membrane receptors in their full biological context, crucial to understanding neuronal signalling. Simultaneous imaging of receptor trafficking and activity in neurons will help to uncover details of the dynamic activity in the brain. This technology is expected to help understand the inner workings of the brain and provide insights into its functioning.

该项目旨在开发纳米荧光体信标和实时、超高灵敏度功能成像，以提供大脑图像。时间选通检测显微镜将为这些纳米磷光体提供卓越的光学对比度。纳米磷的抗体靶向将对单个 AMPA 膜受体在其完整的生物学背景下进行成像，这对于理解神经元信号传导至关重要。对神经元中受体运输和活动的同步成像将有助于揭示大脑中动态活动的细节。这项技术有望帮助了解大脑的内部运作并提供对其功能的见解。