Synaptic plasticity in sex steroid-sensitive motoneurons.

性类固醇敏感运动神经元的突触可塑性。

• 批准号: 01540621
• 负责人: MATSUMOTO Akira
• 金额: $ 1.28万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540621/
• 关键词: Gap junction; connexin 32; mRNA; in situ hybridization; androgen; rat; lumbar spinal cord; motoneuron; インサイチュ・ハイブリダイゼイション; 新生ラット脳; ギャップ結合蛋白のmRNA; 肝臓組織; 脳組織

Gap junctions are considered to play an important role in metabolic and electrical coupling between neurons. We studied androgenic influence on the expression of the mRNA for gap junction protein in the androgen-sensitive motoneurons in the spinal nucleus of the bulbocavernosus(SNB)by using in situ hybridization histochemistry. A complementary DNA specific for the mRNA for r liver gap junction protein(connexin 32, a gift from Dr. D. A. Goodenough, Harvard Medical Sch was applied to in situ hybridization.1. Cellular localization of gap junction mRNA was examined in the neonatal male rat brai Autoradiographic signals for connexin 32 mRNA were found to distribute in various regions of the brain such as parietal cortex, hippocampus, thalamus/striatum and hypothalamus. These signals were localized on neuronal cells. Immunohistochemical and ultrastructural observations of the hippocampus supported the presence of gap junctions in the examined region.2. Cellular localization of gap junction m … More RNA was examined in the lumbar spinal cords of adult male rats. Autoradiographic-signals for connexin 32 mRNA were found to be localized on the somata and proximal dendrites of motoneurons in the SNB, dorsolateral nucleus(DLN)and retrodorsolateral nucleus(RDLN).3. Adult male rats were castrated and implanted with Silastic tubes containing testosterone or nothing. Animals were sacrificed 4 weeks later. The removal of androgen by castration dramatically reduced the expression of gap junction mRNA in the androgen-sensitive SNB motoneurons, whereas this change was prevented by testosterone treatment. On the contrary, castration or testosterone treatment did not induce any changes in the expression level of gap junction mRNA in the androgen-insensitive RDLN motoneurons. These results suggest that androgen influences the expression of gap junction gene in the SNB motoneurons and may provide evidence for the molecular mechanisms of hormonally induced synaptic plasticity in the SNB motoneurons. Less

缝隙连接被认为在神经元之间的代谢和电耦合中起重要作用。应用原位杂交组织化学方法研究了雄激素对球海绵体肌脊核（SNB）内雄激素敏感运动神经元缝隙连接蛋白mRNA表达的影响。一种特异于肝脏间隙连接蛋白（连接蛋白32，由D. A.应用Goodenough，哈佛医学院的方法进行原位杂交.用放射自显影法检测了新生雄性大鼠脑内缝隙连接mRNA的细胞定位。结果表明，缝隙连接蛋白32 mRNA在顶叶皮质、海马、丘脑/纹状体和下丘脑等脑区均有分布。这些信号定位在神经元细胞上。海马的免疫组织化学和超微结构观察支持缝隙连接在检查区域的存在.缝隙连接的细胞定位 ...更多信息 在成年雄性大鼠的腰脊髓中检查RNA。缝隙连接蛋白32 mRNA的放射自显影信号定位于SNB、背外侧核（DLN）和背外侧后核（RDLN）运动神经元的胞体和近端树突.将成年雄性大鼠阉割并植入含有睾酮或不含睾酮的硅橡胶管。4周后处死动物。去除雄激素去势显着降低了雄激素敏感的SNB运动神经元的间隙连接mRNA的表达，而这种变化被阻止睾酮治疗。相反，去势或睾酮处理并没有引起雄激素不敏感的RDLN运动神经元的间隙连接mRNA的表达水平的任何变化。这些结果提示雄激素影响SNB运动神经元缝隙连接基因的表达，为雄激素诱导SNB运动神经元突触可塑性的分子机制提供了证据。少

项目成果

松本 明: "ギャップ結合の可塑性" 生体の科学. 40. 635-639 (1989)

Akira Matsumoto：“间隙连接的可塑性”生物科学。40。635-639（1989）

Matsumoto, A., Arai, Y., Urano, A. and Hyodo, S.: "Androgen regulates gap junction mRNA expression in androgensensitive motoneurons in the rat spinal cord." Neurosci. lett.131. 159-162 (1991)

Matsumoto, A.、Arai, Y.、Urano, A. 和 Hyodo, S.：“雄激素调节大鼠脊髓中雄激素敏感运动神经元的间隙连接 mRNA 表达。”

Matsumoto,A.et al.: "Androgen regulates gap junction mRNA expression in androgenーsensitive motoneurons in the rat spinal cord." Neurosci.Lett.131. 159-162 (1991)

Matsumoto, A. 等人：“雄激素调节大鼠脊髓中雄激素敏感运动神经元的间隙连接 mRNA 表达。Neurosci.Lett.131 (1991)。”

Matsumoto, A., Arnold, A. P. and Micevych, P. E.: "Gap junctions between lateral spinal motoneurons in the rats." Brain Res.495. 362-366 (1989)

Matsumoto, A.、Arnold, A. P. 和 Micevych, P. E.：“大鼠外侧脊髓运动神经元之间的间隙连接。”

Matsumoto, A., Arai, Y., Urano, A. and Hyodo, S.: "Cellular localization of gap junction mRNA in the neonatal rat brain." Neurosci. Lett.124. 225-228 (1991)

Matsumoto, A.、Arai, Y.、Urano, A. 和 Hyodo, S.：“新生大鼠脑中间隙连接 mRNA 的细胞定位”。