Reconstruction of circadian gene network by molecular LEGO
Reconstruction of circadian gene network by molecular LEGO
批准号:
17570134
负责人:
MATSUMOTO Akira
金额:
$2.3万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2006
中文摘要
用“分子乐高”方法在果蝇S2细胞中重建了由人工嵌合基因组成的基因-蛋白质网络。模拟果蝇昼夜节律系统的双反馈环表现出一个相对稳定的周期为4-6小时的振荡。分析了对振荡的稳定性和周期有显著影响的参数,并对每个域中发生突变的成分进行了分析。负环成分信使核糖核酸半衰期的长短对振荡的稳定性有很大的影响。半衰期越短,节律性越稳定。然而,在负性因子中改变信使核糖核酸的半衰期并没有发现任何显著的周期变化。负因子在半衰期内2~16小时的蛋白质降解对振荡的稳定性和周期均无影响。当负因子的蛋白质半衰期与正因子的半衰期相似时,振荡变得稳定。负性因子的核局部化是产生周期性的一个重要参数,因为它的效率改变了重建节律的周期。此外,负性因子的核定位效率越低,就不会导致节律性。消极因素和积极因素之间的比例也很重要。当比例为1:5或5:1时,与它们为偶数时相比,一个给定的周期延长。令人惊讶的是,重建振荡在20到25摄氏度的范围内显示出温度补偿。
英文摘要
Gene-protein networks composed of artificial chimeric genes were reconstructed by "molecular LEGO" method in Drosophila S2 cell. A double feedback loop mimicking Drosophila circadian system showed a relatively stable oscillation with 4-6 hrs in period. Parameters which significantly influences to stability and period of the oscillation were analyzed with the altered components having mutation in each domain. The half life of mRNA of a negative loop component, hereafter called as a negative factor, drastically affects to a stability of oscillation. When the half-life becomes shorter, the rhythmicity becomes more stable. However, any significant change in period was not found by changing the half-life of mRNA in a negative factor. The protein degradation of a negative factor in the range from 2 to 16 hrs in its half-life had no effect on either stability or period of the oscillation. When the protein half-life of a negative factor was similar to that of a positive one, the oscillation becomes stable. The nuclear localization of negative factor was found as an important parameter to generate a periodicity because its efficiency changed the period of the reconstructing rhythm. Moreover, the less efficiency of nuclear localization of a negative factor induced no rhythmicity. The ratio between negative and positive factor is also important. A given period lengthened when the ratio is 1:5 or 5:1 as compared to when they were even. Surprisingly, the reconstructing oscillation showed a temperature-compensation in its period in the range between 20 and 25 C.
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Genome-wide screenings for circadian clock genes in Drosophils.
果蝇生物钟基因的全基因组筛选。
DOI:
--
发表时间:
2006
期刊:
Sleep and Biological Rhythms 4
影响因子:
--
作者:
[M.Sohda, Y.Misumi et al., Akira Matsumoto]
通讯作者:
Akira Matsumoto
A functional genomics strategy reveals clockwork orange as a transcriptional in Drosophila circadian clock.
功能基因组学策略揭示了发条橙在果蝇生物钟中的转录作用。
DOI:
--
发表时间:
2007
期刊:
Gene and Development 21(in press)
影响因子:
--
作者:
[Higuchi, Y., Miura, T., Kajimoto, T., Ohta, Y., Ohta Y, Matsumoto A. et al.]
通讯作者:
Matsumoto A. et al.
Temperature cycles drive Drosophila circadian oscillation in constant light that otherwise induces behavioural arrhythmicity.
温度循环会在恒定的光照下驱动果蝇的昼夜节律振荡,否则会导致行为心律失常。
DOI:
--
发表时间:
2005
期刊:
Europian Journal of Neuroscience 22
影响因子:
--
作者:
[M.Sohda, Y.Misumi, S.Yoshimura, N.Nakamura, T.Fusano, S.Sakisaka, S.Ogata, J.Fujimoto, N.Kiyokawa, Y.Ikehara., Okumura et al., Yoshii et al.]
通讯作者:
Yoshii et al.
DOI:
10.1016/j.ydbio.2004.11.021
发表时间:
2005-02-15
期刊:
DEVELOPMENTAL BIOLOGY
影响因子:
2.7
作者:
[Okumura, T, Matsumoto, A, Murakami, R]
通讯作者:
Murakami, R
DOI:
--
发表时间:
2006
期刊:
Sleep Biol.Rhythms 4
影响因子:
--
作者:
[Sumiyoshi M, Shimohigashi M, et. al., Matsumoto A]
通讯作者:
Matsumoto A
共 6 条
Smart-gel based synthetic artificial pancreas
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Posttranscriptional regulation of clock genes by ABC protein
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Development of Integrated Transistor Based device for Monitoring of Enzymatic Activities as Next-generation Platform for Apoptosis Pathways Analysis
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Civil Society, Nationstate and War in Modern Germany
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Analysis of the function of human brain carboxypeptidase B and its diagnostic significance in dementia of Alzheimer's type
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资助金额:$2.83万
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Nationstate and Identiy-Complex in European History
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Clarification of significance of human brain carboxypeptidase B in cerebrospinal fluid as a diagnostic marker of dementia
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资助金额:$2.3万
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依托单位:
INNOVATION FOR TISSUE-SPECIFIC RADIO-SENCITIZATION OF SELECTIVE INHIBITOR OF CYCLO-OXYGENASE 2 (COX-2)
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EXPRESSION OF NEUROTROPHIC FACTORS RELATED TO PLASTICITY OF MOTONEURONS
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French Revolution and Modern Europe - Empire and Nationstate -
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批准号:12410098
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ANALYSIS OF FUNCTION OF HUMAN BRAIN CARBOXYPEPTIDASE B-SPECIFIC C14-MODULE
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Effect of Thermal Therapy on Clinical Status, Neurohumoral Factors and Endothelial Function in Patients With Chronic Heart Failure
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Regulatory mechanisms of expression of nuclear receptor coactivators in motoneurons
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APPLICATION OF B-SECRETASE FOR PRESYMPTOMATIC DIAGNOSIS OF ALZHEIMERS DISEASE
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SIGNIFICANCE OF THE 68-K SERINE PROTEASE IN SERUM AS A MARKER PROTEIN FOR BRAIN AGING
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负责人:MATSUMOTO Akira
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Molecular mechanisms of androgen receptor expression
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批准号:08640850
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资助金额:$1.41万
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依托单位:
Function analysis of projections on the surface of Chlamydia trachomatis organisms, major pathogens of sexually transmitte diseases
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批准号:07671759
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.34万
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财政年份:1995
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依托单位:
Analysis of the mechanisms of beta-amyloid accumulation by 68kDa serine protease with beta-secretase activity
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批准号:07670173
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.15万
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负责人:MATSUMOTO Akira
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依托单位:
Molecular basis of synaptic plasticity
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批准号:06640869
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.41万
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财政年份:1994
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依托单位:
Molecular basis of neuronal plasticity
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批准号:04640692
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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负责人:MATSUMOTO Akira
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依托单位:
海外基金