Role of calcium in abscission of citrus

钙在柑橘脱落中的作用

• 批准号: 01560038
• 负责人: IWAHORI Shuichi
• 金额: $ 1.09万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560038/
• 关键词: abscission; antimonate method; brassinolide; Calamondin; calcium ion; IAA; trifluoperazine; カルシウム; 柑橘; 顕微化学; 細胞内カルシウム検出; ピロアンチモン酸; ブラシノライド

To study the role of calcium in abscission of citrus, 1) interaction of brassinolide (BR) and trifluoperazine (TFP), an. initiator of calamondin- calcium complex in abscission, 2) subcellular localization of calcium during senescence and abscission of leaf explants, were investigated. BR, a novel plant growth-regulating steroidal lactone, markedly retarded the abscission of leaf explants of Calamondin (Citrus maduretisis Lour.), when dissolved in itiator and fed through the petiole. BR was effective at concentrations as low as 0.021muM, and showed a stronger effect than IAA which also retarded abscission. TFP, an inhibitor of the calmodulin-calcium complex, accelerated abscission, and this acceleration could be counteracted by a simultaneous addition of IAA or BR, the effect of IAA being stronger. This suggests the mechanism of retardation of abscission by BR may be different from that of IAA. BR and IAA also markedly retarded and TFP accelerated the abscission of fruitlet explants of … More Calamondin.Subcellular localization of calcium in the cells of abscission layer and adjacent parencliyma tissue was investigated electronmicroscopically by Slocum and. Roux's antimonate precipitation method using leaf explants of Calamondin. By comparing the specimens which were fixed with fixatives with or without potassium antimonate, calcium was detected as electron-dense small particles and that was thought to be free calcium ion. In fresh or IAA-treated explants only a few particles were present in the cells of abscission layer maiixly along plasmalemma. In the explants which uiiderwetit some senescence, more particies were present in these cells along pasmalemma but also in, cytoplasm and middle lamella of tile cell wall. In abscising explants which were treated with trifluoperazine(TFP), abundant particles were present in the degenerated middle lamella. These results show that the amount, type and subcellular localization of calcium present in the cells of abscission layer closely related to the abscission. Less

为研究钙在柑桔果实衰老过程中的作用，采用1）油菜素内酯（BR）与三氟拉嗪（TFP）的互作效应，以及钙离子对柑桔果实衰老的影响。2）叶片外植体衰老和脱落过程中钙离子亚细胞定位。BR是一种新的植物生长调节甾体内酯，它能显著地延缓Calamondin（Citrus maduretisisLour.）当溶解在引发剂和饲料通过叶柄。BR在低至0.021 μ M的浓度下就有效，并且表现出比IAA更强的作用，后者也延迟脱落。钙调素-钙复合物的抑制剂TFP能加速脱落，同时加入IAA或BR可抵消这种加速作用，IAA的作用更强。这表明BR延缓水稻脱落的机理可能与IAA不同。BR和IAA也显著地延缓了幼果外植体的脱落，而TFP则促进了幼果外植体的脱落 ...更多信息 Calamondin. Slocum和.使用Calamondin的叶外植体的Roux锑酸盐沉淀法。通过比较用含锑酸钾和不含锑酸钾的固定剂固定的标本，检测到钙为电子致密的小颗粒，认为这是游离钙离子。在新鲜的或IAA处理的外植体中，只有少数颗粒存在于细胞中，主要沿着质膜分布。在未衰老的外植体中，细胞壁的沿着膜、细胞质和中层中有较多的颗粒。在用三氟拉嗪（TFP）处理的外植体中，大量的颗粒存在于退化的中间层中。这些结果表明，游离层细胞内钙的含量、类型及亚细胞定位与游离密切相关。少

项目成果

Iwahori,S.: "Role of calcium in abscission of citrus" Proc.Int.Citrus Symp hend in Guangzhou,China.

Iwahori,S.：“钙在柑橘脱落中的作用”Proc.Int.Citrus Symp hend，中国广州。

Iwahori,S.,S.Tominaga and S.Higuchi: "Retardation of abscission of citrus leaf and fruitlet explants brassinolide." Plant Growth Regulation. 9. 119-125 (1990)

Iwahori，S.，S.Tominaga 和 S.Higuchi：“油菜素内酯延缓柑橘叶和小果外植体的脱落。”

Iwahori,S.,S.Tominaga and S.Higuchi: "Retardation of abscission of citrus leaf and fruitlet explants by brassinolide" Plant Growth Regulation. (1990)

Iwahori，S.，S.Tominaga 和 S.Higuchi：“油菜素内酯延迟柑橘叶和小果外植体的脱落”植物生长调节。

Iwahri,S.: "Role of calcium in abscission of citrus." Proc.Int.Citrus Symp.(1991)

Iwahri,S.：“钙在柑橘脱落中的作用。”

Iwahori, S., S. Tominaga and S. Higuchi: "Retardation of abscission of citrus leaf and fruitlet explants by brassionolide." Plant Growth Regulation. 9. 119-125 (1990)

Iwahori, S.、S. Tominaga 和 S. Higuchi：“芸苔素内酯延缓柑橘叶和小果外植体的脱落。”