Molecular Role of Calcium and Boron for Pollen Germination of Higher Plants

钙和硼对高等植物花粉萌发的分子作用

基本信息

  • 批准号:
    01560075
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1989
  • 资助国家:
    日本
  • 起止时间:
    1989 至 1990
  • 项目状态:
    已结题

项目摘要

Culture conditions for pollen germination of Lilium longiflorum on a synthetic medium were established: 1 mM CaCl_2, 1mM H_3BO_3 and 0.3 M sucrose were required for an agar medium, 0.1 mM Ca(NO_3)_2, 0.5mM H_3BO_3 and 0.3 M sucrose for a liquid medium, with a pollen density of 0-3 mg/ml. Microanalyses of calcium and boron using X-ray energy spectrometer were not successful because of difficulty in pollen tube sample preparation.EDTA and EGTA, a chelator, and H-7 as protein kinase C inhibitor, stimulated pollen germination at the early stage of germination, whereas protein synthesis inhibitor cycloheximide (CHI) inhibited the germination. However, treatment of pollen with CHI followed by EDTA treatment stimulated the germination rather than inhibited it. This suggests that protein actions required for the germination can be replaced with EDTA and that calcium may interact with cell wall and/or plasma membrane.Fatty acid composition of phosphatidylinositol (PI) changed during the pollen germination, distribution of unsaturated fatty acids such as 18:2 and 18:3 reduced while percentage of 16:0 increased. This change with PI was different from that of phosphatidylcholine. Although detection of inositol triphsphate (IP_3) has not been successful in the pollen germination system in our experiment, the above result suggests that PI-mediated calcium regulation system may play an important role during pollination.To examine calcium effect on cell wall loosening, cell wall degrading enzgme activities were assayed. Significant activities of polygalacturonase and beta-1, 3-glucanase were detected. However, addition of calcium to the medium neither stimulated nor inhibited these enzyme activitie. Now we successfully purified beta-1, 3-glucanase from L. longiflorum leaves to administrate to rabbit for preparation of anti-beta-1, 3-glucanase serum and immuno-histological investigation of pollen termination in connection with calcium and boron action.
麝香百合(Lilium longiflorum)花粉在合成培养基上萌发的培养条件为:琼脂培养基需要1mMCaCl_2,1mMH_3BO_3和0.3M蔗糖;液体培养基需要0.1mMCa(NO_3)_2,0.5mMH_3BO_3和0.3M蔗糖;用X射线能谱仪测定花粉中的钙和硼,由于样品制备困难而未能成功,螯合剂EDTA和EGTA及蛋白激酶C抑制剂H-7在花粉萌发早期促进花粉萌发,而蛋白合成抑制剂放线菌酮(CHI)则抑制花粉萌发。然而,用CHI处理花粉,然后用EDTA处理花粉,可以刺激而不是抑制花粉的萌发。这表明萌发所需的蛋白质作用可以被EDTA取代,并且钙可能与细胞壁和/或质膜相互作用。在花粉萌发过程中,磷脂酰肌醇(PI)的脂肪酸组成发生变化,18:2和18:3等不饱和脂肪酸的分布减少,而16:0的百分比增加。PI的这种变化与磷脂酰胆碱不同。本实验还没有成功地检测到三磷酸肌醇(inositol triphspate,IP_3)在花粉萌发过程中的存在,但上述结果表明,PI介导的钙调节系统在授粉过程中可能起着重要作用。多聚半乳糖醛酸酶和β-1,3-葡聚糖酶的活性显著。然而,在培养基中添加钙既不刺激也不抑制这些酶的活性。本实验成功地从L.麝香草叶片给药家兔制备抗β-1,3-葡聚糖酶血清,并对花粉终止与钙、硼作用的关系进行免疫组织学研究。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
J.Sekiya,Y.Nakata,K.Fujii and M.Tada: "Purification of βー1,3ーglucanase from Liliumu longiflorum leaves" Agric.Biol.Chem.,.
J.Sekiya、Y.Nakata、K.Fujii 和 M.Tada:“从百合叶中纯化 β-1,3-葡聚糖酶”Agric.Biol.Chem.,。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
J. Sekiya, Y. Nakata, K. Fujii and M. Tada: "Purification of beta-1, 3-glucanase from Lilium longiflorum leaves." Age. BIol. Chem.
J. Sekiya、Y. Nakata、K. Fujii 和 M. Tada:“从长花百合叶中纯化 β-1, 3-葡聚糖酶。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

SEKIYA Jiro其他文献

SEKIYA Jiro的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('SEKIYA Jiro', 18)}}的其他基金

γ-Glutamyltransferases in higher plants and catabolism of glutathiones
高等植物中的γ-谷氨酰转移酶和谷胱甘肽的分解代谢
  • 批准号:
    18580060
  • 财政年份:
    2006
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Multiforn of γ-Glutamyltransfemse in Higher Plants and Cysteine Recycle
高等植物中γ-谷氨酰转移酶的多种形式及半胱氨酸的回收
  • 批准号:
    14560052
  • 财政年份:
    2002
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on Catabolism of Glutathione and Recycle System of Cysteine in Higher Plants
高等植物谷胱甘肽分解代谢及半胱氨酸回收系统的研究
  • 批准号:
    12660059
  • 财政年份:
    2000
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Biochemistry and molecular biology of glutathione in higher plants
高等植物谷胱甘肽的生物化学和分子生物学
  • 批准号:
    09660062
  • 财政年份:
    1997
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular Analysis of Rice Mitochondrial F_0F_1-ATPase and Effect of Mineral Element Deficienct on the Enzyme
水稻线粒体F_0F_1-ATP酶的分子分析及矿质元素缺乏对酶的影响
  • 批准号:
    04660070
  • 财政年份:
    1992
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Digitally-Integrated Smart Sensing of Diverse Airborne Grass Pollen Sources
多种空气传播草花粉源的数字集成智能传感
  • 批准号:
    DP240103307
  • 财政年份:
    2024
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Discovery Projects
Construction of a system for the automatic pollen identification by AI and evaluation of composite pollination networks.
人工智能花粉自动识别及复合授粉网络评估系统的构建
  • 批准号:
    23K14273
  • 财政年份:
    2023
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The role of ELMOD family proteins and their genetic network in the development of specialized membrane domains on the Arabidopsis pollen surface
ELMOD家族蛋白及其遗传网络在拟南芥花粉表面特殊膜结构域发育中的作用
  • 批准号:
    2240972
  • 财政年份:
    2023
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Standard Grant
Establishment of new pollen culture system based on single-cell sorting
基于单细胞分选的新型花粉培养体系的建立
  • 批准号:
    23K17987
  • 财政年份:
    2023
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Principles of Molecular Action Mechanisms in Two-Step Ovular Guidance for the Pollen Tube
花粉管两步卵引导的分子作用机制原理
  • 批准号:
    22H04980
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Cellular signalling mechanisms during pollen-pistil interactions in Brassicaceae
十字花科花粉-雌蕊相互作用过程中的细胞信号机制
  • 批准号:
    RGPIN-2020-05301
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Discovery Grants Program - Individual
Determination of ultra and fine particles containing pollen cross-allergen, plastics and toxic compounds in urban atmosphere
城市大气中含有花粉交叉过敏原、塑料和有毒化合物的超细颗粒的测定
  • 批准号:
    22H03747
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
From anther to atmosphere: building a better pollen forecast for human health (ref:4250)
从花药到大气:为人类健康建立更好的花粉预测(参考:4250)
  • 批准号:
    2698476
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Studentship
Cellular regulation of pollen compatibility and fertility in the Brassicaceae
十字花科花粉相容性和育性的细胞调节
  • 批准号:
    RGPIN-2018-04348
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Discovery Grants Program - Individual
Evolution of flower colour in a pollen limited environment
花粉有限环境中花色的演变
  • 批准号:
    574222-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 1.28万
  • 项目类别:
    University Undergraduate Student Research Awards
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了