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Physiological Functions and Transport of Iron Compound in Several Animal Species

几种动物中铁化合物的生理功能和转运

基本信息

批准号：
01560298
负责人：
TSUDA Tsuneyuki
金额：
$ 1.02万
依托单位：
Tokyo University of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560298/
关键词：
Iron Intestinal absorption Chelating compound Transferrin Sheep New born lamb Bipyridine Phenanthroline キレ-ト剤

项目摘要

I. Transport of iron compounds though small intestine(1) Iron plays several important roles in the animal body. In the present study, bipyridine and phenanthroline which are both easily chelated with Fe^<2+> and Fe^<3+> were used to know the rate and mechanism of absorption form from small intestine in several species of animals. Inverted small intestinal sacs obtained from rabbits, guinea pigs and quails were incubated with above mentioned Fe chelated agents. Ferrous compounds were more easily absorbed into the lumen of intestine than ferric ones. On the contrary, ferric compound remained inside of the tissue walls was higher in amount than that of ferrous ones. Fe chelated peptide used as control substance exhibited least both absorbed and intratissue remained amounts of iron.(2) To know the absorption mechanism of above substances, the results obtained using inverted intestine were compared with those of non inverted ones. The absorbed amounts of iron through inverted intestinal sacs were always higher than those of non inverted ones with a exception of Fe peptide. These results suggest iron absorption through intestinal wall is not simple diffusion but is regulated by active transport mechanism.(3) To know whether or not above chelated compounds act physiologically as Fe carrier in the animal body, Fe^<2+> and Fe^<3+> bipyridines and Fe peptide were introduced per as to sheep. Fe^<2+> bipyridine absorbed and functioned most effectively among tested three compounds. The excreted amounts into urine, however, was least in Fe peptide.II. Serum transferrin (Tf) concentrations in new born lambs and dams(1) Tf is a most important Fe carrier in the animal body. Serum Tf concentrations in new born lambs were relatively high values indicating self-productive ability in their own liver in addition to the supply from their dams.

I.铁化合物在小肠中的转运（1）铁在动物体内起着几种重要的作用。本研究利用易与Fe^<2+>和Fe^<3+>螯合的联吡啶和邻菲咯啉，研究了它们在几种动物小肠中的吸收速率和吸收机制。将兔、豚鼠和鹌鹑的倒置小肠囊与上述铁螯合剂一起孵育。亚铁化合物比三价铁化合物更容易被肠腔吸收。相反，铁化合物留在组织壁内的量高于亚铁化合物。以铁螯合肽为对照，其吸收量和组织内残留量均最少。(2)为了解上述物质的吸收机制，将倒置小肠法和非倒置小肠法的结果进行了比较。倒置肠囊对铁的吸收量除铁肽外均高于非倒置肠囊。提示铁在肠壁的吸收不是简单的扩散，而是受主动转运机制的调节。(3)为了了解这些螯合物在动物体内是否具有生理上的铁载体作用，将Fe^<2+>、Fe^<3+>联吡啶和Fe肽引入绵羊体内。在所测试的三种化合物中，Fe^<2+> bipyridine的吸附和作用效果最好。铁肽经尿排泄量最少。新生羔羊和母畜血清转铁蛋白（Tf）浓度的研究（1）Tf是动物体内最重要的铁载体。新生羔羊血清转铁蛋白浓度相对较高，表明除了来自母羔羊的供应外，其自身肝脏也具有自我生产能力。