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Effect of Sex Steroids on T Cell Differentiation and Maturation in the Thymus.

性类固醇对胸腺 T 细胞分化和成熟的影响。

基本信息

批准号：
01570020
负责人：
SEIKI Kanji
金额：
$ 0.9万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570020/
关键词：
Mouse thymus Reticuloepithelial cell T cell Sex steroid receptor Thymic hormone Scatchard plot Immunohistochemistry FACscan 性ホルモン胸腺細胞胸腺上皮

项目摘要

For recent years we have shown not only the presence of receptors for sex steroids (SH), androgen, estrogen (E) and progestin (P) in the thymus tissue, but also the immunohistochemical localization of these receptors in reticuloepithelial (RE) cells within the tissue in which thymic hormone (thymulin) is also exclusively localized. These findings clearly mean that both SH-containing cells and thymulin producing cells are the same RE cells, suggesting that SH may mediate some immune functions of the thymus through its receptors within the RE cells which produce thymulin to lead T cell differentiation.Female mice of C57BL/6NJcl strain were used. They were either ovariectomized (OVX) alone, OVX-estrogen-treated, or OVX-thymulin-treated. The thymus tissues were excised from each animal, and offered for the following experiments ;1. Scatchard plot analysis for steroid receptors (SR), 2. immunohistochemical staining to localize SR and thymulin, and 3. FACscan analysis to classify T cell subp … More opulation.The mouse thymus possessed specific receptors for E (ER) and P (PR) with high affinities and low capacities. In the ER-stained section, the stained cells were mainly located in the medulla, with dendritic cell processes, presumably RE cells. The cells comprising Hassall's corpuscle were also stained receptor-positive. In the adjacent section, a large number of thymulin-stained cells were localized exclusively in the medulla, with dendritic cell processes, identical with ER-positive RE cells. The cells of Hassall's corpuscle were also thymulin-positive. Tentatively, these results clearly indicate that both ER-containing cells and thymulin-producing cells are the same RE cells, similar to those reported for the rat thymus. E-treatment brought about a striking decrease in thymic tissue weight, whereas thymulin-treatment caused a fairly remarkable increase in weight. In turn, the loss of tissue weight by E depended on a significant decrease in the immature double positive T cell subsets with only a slight increase in the helper/inducer T cells. On the contrary, the gain of tissue weight by thymulin depended on a significant increase in the helper/inducer T cell subsets with no change in other T cell subpopulations.From the present study, it is clearly indicated that E mediates immune function of the thymus through its receptor with in RE cells which produce thymulin to lead differentiation and maturation of T cells. Less

近年来，我们不仅证明了胸腺组织中存在性类固醇（SH）、雄激素、雌激素（E）和孕激素（P）受体，而且还证明了这些受体在胸腺激素（胸腺素）也专门定位的组织内的网状上皮（RE）细胞中的免疫组织化学定位。这些发现清楚地表明，含有SH的细胞和产生胸腺素的细胞是相同的RE细胞，这表明SH可能通过其在RE细胞内的受体介导胸腺的一些免疫功能，产生胸腺素以引导T细胞分化。使用C57BL/6NJcl品系的雌性小鼠。他们要么单独切除卵巢（OVX），要么接受OVX雌激素治疗，要么接受OVX胸腺素治疗。从每只动物身上切下胸腺组织，并提供以下实验；1．类固醇受体 (SR) 的斯卡查德图分析，2. 免疫组织化学染色以定位 SR 和胸腺素，以及 3. FAC 扫描分析以对 T 细胞亚型进行分类。小鼠胸腺具有 E (ER) 和 P (PR) 的特异性受体，具有高亲和力和低容量。 ER染色切片中，染色细胞主要位于髓质，有树突状细胞突起，推测为RE细胞。包含哈萨尔氏小体的细胞也被染色为受体阳性。在相邻切片中，大量胸腺素染色细胞仅位于髓质中，具有树突状细胞突起，与 ER 阳性 RE 细胞相同。哈索尔小体的细胞也呈胸腺素阳性。初步地，这些结果清楚地表明，含有 ER 的细胞和产生胸腺素的细胞是相同的 RE 细胞，与报道的大鼠胸腺细胞相似。电子治疗导致胸腺组织重量显着下降，而胸腺素治疗则导致体重相当显着增加。反过来，E引起的组织重量损失取决于未成熟双阳性T细胞亚群的显着减少，而辅助/诱导T细胞仅略有增加。相反，胸腺素对组织重量的增加依赖于辅助/诱导T细胞亚群的显着增加，而其他T细胞亚群没有变化。从本研究中，清楚地表明E通过其与RE细胞中的受体介导胸腺的免疫功能，RE细胞产生胸腺素以引导T细胞的分化和成熟。较少的