When will functional thymic-gonadal axis be established ?

功能性胸腺-性腺轴何时建立？

• 批准号: 03807002
• 负责人: SEIKI Kanji
• 金额: $ 0.77万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807002/
• 关键词: human fetal thymus; estrogen receptor; serum thymic factor; thymic epithelial cell; Hassall body; thymus-gonad axis; 胎児胸腺; エストロゲン(E); エストロゲンレセプター(ER); ER-mRNA; 胸腺上皮細胞; 胸腺T細胞; 胸腺細胞; エストロゲンレセプタ-; アポプト-シス

This study was carried out to determine when functional 'thymusgonad' axis would be established using human fetal thymus. Thymus tissues regally obtained from 13 to 24 week old fetuses were used for (1) immunohistochemical staining of estrogen receptor (ER), serum thymic factor (FTS) and keratin, and (2) quantitation by ELISA of ER and FTS in the tissue.The findings were as follows ; (1) in 16 week old fetal thymus ER-stained cells appeared exclusively in the medullary area which were confirmed as epithelial cells by keratin-staining. They were also scattered in the cortical area. (2) at the same gestational week, predominantly FTS-stained cells were diffusely distributed throughout the medulla, with some scattered in the cortical area. Their structural features were similar to those of ER-stained cells.The present results may provide evidence for E regulation of development-growth of the fetal thymus at mid-pregnancy through its effect on the epithelial cells via a receptor mechanism that produces FTS to stimulate the proliferation, differentiation and/or selection of T cells.

本研究旨在确定使用人胎儿胸腺建立功能性“胸腺腺”轴的时间。取13 ~ 24周龄胎儿常规胸腺组织(1)免疫组化染色雌激素受体（ER）、血清胸腺因子（FTS）和角蛋白；(2)ELISA法定量组织中雌激素受体（ER）和FTS。调查结果如下：(1) 16周龄胎胸腺er染色细胞仅出现在髓质区，角蛋白染色证实为上皮细胞。它们也分散在皮质区。(2)在同一孕周，主要是fts染色的细胞弥漫性分布在髓质，部分分散在皮质区。其结构特征与er染色细胞相似。目前的研究结果可能为妊娠中期胎儿胸腺发育生长的E调控提供证据，其通过受体机制对上皮细胞的影响，产生FTS刺激T细胞的增殖、分化和/或选择。

项目成果

Kou Sakabe: "Expression of estrogen receptor cells with special reterence to thymulin (FTS)-producing cells in human fetal thyinus during midpreararoy" Medical Science pesearch. 21. 337-341 (1993)

Kou Sakabe：“雌激素受体细胞在人胎儿胸腺中的表达，对胸腺素（FTS）产生细胞具有特殊的依赖性”医学科学研究。

Kou Sakabe: "Expression of estrogen receptor cells,with special reference to thymulin(FTS)-producing cells in human fetal thymus during mid-pregnancy" Medical Science Research. 21. 337-341 (1993)

Kou Sakabe：“雌激素受体细胞的表达，特别是妊娠中期人胎儿胸腺中产生胸腺素（FTS）的细胞”医学科学研究。

Kou Sakabe et al.: "Expression of estrogen receptor cells, with special reference to thymulin (FTS)-producing cells in human fetal thymus during mid-pregnancy" Medical Science Research. 21. 337-341 (1993)

Kou Sakabe 等人：“雌激素受体细胞的表达，特别是妊娠中期人胎儿胸腺中产生胸腺素 (FTS) 的细胞”医学科学研究。