权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Influence of estrogen on reproductive organization in perinatal mouse brain, with special reference to xenoestrogen

雌激素对围产期小鼠大脑生殖组织的影响，特别是异雌激素

基本信息

批准号：
10670032
负责人：
SEIKI Kanji
金额：
$ 0.77万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We have set out to obtain an information of whether high E levels during pregnancy would affect the development of perinatal mouse brain, especially the sex-related area through an estrogen receptor (ER) mechanism. We also examined the influence of habitual smoking during pregnancy on perinatal brain development by ad libitum administration of a nicotine-water solution.We have investigated the effects of administration of estriol (EィイD23ィエD2) and/or nicotine to pregnant mouse on neonatal offspring brain development, using immunohistochemical and radiobiochemical techniques.Estriol caused significant decreases in brain weight and ER concentration after birth, whereas nicotine plus EィイD23ィエD2 brought about no changes in brain weight or ER concentration. Intensities of ER- and neuron-specific enolase (NSE)-immunostainings were both enhanced by EィイD23ィエD2 in the reproductive organization including the preoptic area (POA), anterior to middle medial basal hypothalamus (AMBH) and amygdala (AM) after birth, Nicotine alone did not enhance the ER-immunostain intensity in POA, AMBH or AM, whereas it did enhance the NSE-immunostain intensity in these brain areas. On the contrary, simultaneous administration of nicotine and EィイD23ィエD2 brought about the reverse effect of ER- and NSE-immunostain intensities.These results indicate that estrogen treatment during the perinatal period influences, through its receptor, the development and growth of the reproductive organization in the fetal brain. The influence of nicotine needs to be further elucidated.

我们已经着手获得的信息是否在怀孕期间高E水平会影响围产期小鼠脑的发育，特别是通过雌激素受体（ER）机制的性相关领域。我们还通过随意给予尼古丁水溶液，研究了妊娠期习惯性吸烟对围产期脑发育的影响。用免疫组织化学和放射生物化学技术研究了雌三醇（E3）D23受体拮抗剂（E3 D23）和尼古丁（N2）对孕鼠仔鼠脑发育的影响。而尼古丁+E β D23 β D2对脑重量和ER浓度无影响。结果表明，尼古丁D23、D24、D25均能增强生殖器官视前区（POA）、下丘脑中内侧基底前区（AMBH）和杏仁核（AM）的ER和NSE免疫染色强度，尼古丁单独作用不增强POA、AMBH和AM的ER免疫染色强度，但能增强这些脑区的NSE免疫染色强度。相反，同时给予尼古丁和雌二醇D23受体D2则使ER和NSE的免疫染色强度相反，这些结果表明，围产期雌激素治疗通过其受体影响胎脑生殖组织的发育和生长。尼古丁的影响需要进一步阐明。