Target Specificity of Projections from Postganglionic Sympathetic Neurons Innervated by Enkephalin Containing Preganglionic Fibers.

由含有脑啡肽的节前纤维支配的节后交感神经元的投射的目标特异性。

• 批准号: 01570029
• 负责人: MASUKO Sadahiko
• 金额: $ 1.34万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570029/
• 关键词: Preganglionic sympathetic neuron; Enkephalin containing basket-like terminal; Stellate ganglion; VIP; CGRP; SP containing postganglionic neuron; Sweat gland innervation; Non-catecholaminergic ganglionic neuron; Target specific innervation; 籠状エンケフ

It has been known that neurons of sympathetic nervous system contain many kinds of neuropeptides in addition to the classical neurotransmitter such Noradrenaline (NA) or acetylcholine. In the present study we investigated specific connections coded with certain neuropeptides between sympathetic nerve cells and their targets in the dog. By use of the fast blue tracing technique and immunohistochemistry, it was revealed that :1. Enkephalin (ENK)-Immunoreactive (IR) fibers were selectively distributed in the stellate and L_6-S_1 sympathetic ganglia, and were originated from preganglionic neurons in the intermediolateral nucleus of spinal cord.2. The ENK-1R fibers surrounded Vasoactive Intestinal Peptide (VIP)/Calcitonin Gene-Related Peptide (CCRP)/Substance P (SP)-IR non-NA ganglionic cells and formed a basket-like terminal.3. Many ENK-1R terminals formed direct contacts with somata of postganglionic neurons and also made asymmetrical axo-dendritic synapses.4. The VIP/CGRP/SP-IR postganglionic neurons in the stellate ganglion innervated selectively to the sweat glands of the footpads.Thus, a target specific connection, i. e., the ENK containing preganglionic neurons -> VIP/CGRP/SP containing postganglionic neurons -> sweat glands, was demonstrated.In another experiment, we demonstrated target-specific projections of intrinsic ganglionic neurons with different chemical codes in the canine larynx.

已知交感神经系统的神经元除了含有去甲肾上腺素（NA）、乙酰胆碱等经典神经递质外，还含有多种神经肽。在本研究中，我们调查了特定的连接编码的某些神经肽之间的交感神经细胞和它们的目标在狗。应用快速蓝示踪技术和免疫组织化学方法，结果表明：1.脑啡肽（ENK）免疫反应（IR）纤维选择性分布于星状神经节和L_6-S_1交感神经节，起源于脊髓中间外侧核的节前神经元. ENK-1 R纤维围绕血管活性肠肽（VIP）/降钙素基因相关肽（CCRP）/P物质（SP）-IR非NA神经节细胞，形成篮状终末.许多ENK-1 R终末与节后神经元胞体形成直接接触，并形成不对称的轴-树突触.星状神经节内的VIP/CGRP/SP-IR节后神经元选择性地支配足垫的汗腺，从而形成了一种靶特异性的联系，即：例如，在另一个实验中，我们证明了具有不同化学编码的狗喉内神经节神经元的靶特异性投射。

项目成果

Sadahiko Masuko et al.: "Targetーspecific projections of intrinsic ganglionic neurons with different chemical codes in the canine larynx" Neuroscience Research. 9. 270-278 (1991)

Sadahiko Masuko 等人：“犬喉中具有不同化学密码的内在神经节神经元的目标特异性投射”《神经科学研究》9. 270-278 (1991)。

Sadahiko Masuko: "Target-specific innervation of ENK immunoreactive nerve terminals in the canine sympathetic ganglion."

Sadahiko Masuko：“犬交感神经节中 ENK 免疫反应性神经末梢的目标特异性神经支配。”

Sadahiko Masuko: "Targetーspecific innervation of ENK immunoreactive nerve terminals in the canine sympatheic ganglion"

Sadahiko Masuko：“犬交感神经节中 ENK 免疫反应性神经末梢的目标特异性神经支配”

Sadahiko Masuko: "Target specific projections of intrinsic ganglionic neurons with different chemical codes in the canine larynx"

Sadahiko Masuko：“针对犬喉中具有不同化学密码的内在神经节神经元的特定投射”

Sadahiko Masuko: "Targetーspecific innervation of ENK immunoreactive nerve terminals in the canine sympathetic ganglion"

Sadahiko Masuko：“犬交感神经节中 ENK 免疫反应性神经末梢的目标特异性神经支配”