Neuronal circuitry and nerve terminals regulating the sacral parasympathetic preganglionic neurons

调节骶副交感节前神经元的神经回路和神经末梢

• 批准号: 12680734
• 负责人: MASUKO Sadahiko
• 金额: $ 2.3万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680734/
• 关键词: Sacral spinal cord; Parasympathetic preganglionic neuron; Visceral afferent nerve; Inter Neuron; Spinal cord descending fiber; Neuropeptide; Vesicular acetylcholine transporter; Vanilloid Reception 1; Vanilloid Receptor 1(VR1); 脳幹; カテコラミン; モノアミ

To clarify the neural regulation to parasympathetic preganglionic neurons, nerve fibers and terminals distributing around sacral preganglionic neurons of the rat were investigated by using immunohistochemistry combined with axonal racing and nerve transection experiments, and the following results were obtained.1. About 50-60% of the sacral preganglionic neurons were immunoreactive for NO synthase.2-1). In the sacral spinal cord, substance P (SP)-, calcitonin gene-related peptide (CGRP)-, vesicular acetylcholine transporter (VAChT)-, and vasoactive intestinal polypeptide (VIP)-immunoreactive fibers extending from Lissauer's tract along the lateral edge of the dorsal horn to the autonomic nucleus were found, and fibers immunoreactive for SP and CGRP formed a dense networks of nerve terminals around the preganglionic cells.2). Although, both lumbar and sacral preganglionic neurons received enkephalin (ENK)-immunoreactive nerve fibers from the dorsal central gray, ENK fibers in the sacral … More spinal cord also contained neuropeptide Y (NPY) and formed nerve terminals mainly distributing outside of the CGRP/SP nerve networks.3). Lumbar sympathetic preganglionic neurons received many serotonin (5-HT)- and tyrosine hydroxylase (TH)-immunoreactive descending fibers. In contrast, 5-HT and TH fibers around sacral preganglionic neurons were scarce and. Did not showed selective distribution.3. These fibers did not disappear after the transection of the L4, L5 spinal cord, suggesting that they are of intrinsic or of peripheral origin.4. Transection experiments of the spinal nerve revealed that VAChT, VIP, SP and CGRP fibers were of peripheral origin, and VAChT and VIP fibers came from the myenteric neurons in the most distal potion of the rectum.5. In the pelvic visceral sensory system, the expression of vanilloid reception 1 (VR1) was restricted to the afferents from the ureter and fundus of urinary bladder, the superficial dorsal horn and fibers in the lateral collateral pathway extending along the lateral edge of the dorsal horn into the parasympathetic preganglionic nucleus, suggesting that these VR1-positve afferents were involved in regulation of the sacral preganglionic neurons. Less

为阐明神经对副交感节前神经元的神经调节作用，采用免疫组织化学方法，结合轴突竞速和神经横断实验，对大鼠骶神经节前神经元周围的神经纤维和终末进行了研究。约50-60%的神经节前神经元对一氧化氮合酶呈免疫反应。在脊髓内，P物质(SP)、降钙素基因相关肽(CGRP)、囊泡乙酰胆碱转运体(VAChT)和血管活性肠肽(VIP)免疫反应纤维从Lissauer氏束沿背角外侧缘延伸至自主神经核，SP和CGRP免疫反应纤维在节前细胞周围形成致密的神经末梢网络。虽然腰部和骶部节前神经元都接受来自背侧中央灰质的脑啡肽免疫反应神经纤维，但在骶骨…更多的脊髓还含有神经肽Y(NPY)，形成的神经末梢主要分布在CGRP/SP神经网络之外。腰交感神经节前神经元接受大量5-羟色胺(5-羟色胺)和酪氨酸羟化酶(TH)免疫反应阳性下行纤维。5-羟色胺和TH纤维较少，分布于神经节前神经元周围。未表现出选择性分布。这些纤维在L4、L5脊髓横断后并未消失，提示它们是固有的或外周起源的。脊神经横断实验显示，Vacht、VIP、SP和CGRP纤维均为外周来源，Vacht和VIP纤维来自直肠最远端的肌间神经元。在盆腔内脏感觉系统中，香草素受体1(VR1)的表达仅限于来自输尿管和膀胱底、浅背角的传入纤维和沿背角外侧缘延伸至副交感节前核的侧支通路中的纤维，提示这些VR1阳性传入参与了对骶神经节前神经元的调节。较少

项目成果

Li M-Z, Masuko S: "Target specific organization and neuron types of the dog pelvic ganglia : a retrograde-tracing and immunohistochemical study"Arc. Histol. Cytol. 64(3). 267-280 (2001)

Li M-Z、Masuko S：“狗盆腔神经节的目标特定组织和神经元类型：逆行追踪和免疫组织化学研究”Arc。

Li M-Z, Masuko S: "Target specific organization and neuron types of the dog pelvic ganglia : a retrograde-tracing and immunohistochemical study"Arc. Histol. Cytol.. 64(3). 267-280 (2001)

Li M-Z、Masuko S：“狗盆腔神经节的目标特定组织和神经元类型：逆行追踪和免疫组织化学研究”Arc。

Li M.Z. and Masuko S.: "Target specific organization and neuron types of the dog pelvic ganglia: a retrograde-tracing and immunohistochemical study"Arc. Histol. Cytol.. 64(3). 267-280 (2001)

李明志