Non-descending inputs to the sympathetic preganglionic neuron and their synaptic structures.

交感神经节前神经元及其突触结构的非下降输入。

• 批准号: 07680824
• 负责人: MASUKO Sadahiko
• 金额: $ 1.47万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680824/
• 关键词: Sympathetic post gangionic neuron; Spinal cord; gangliospinal projection; Intermediolateral nucleus; Retrograde neuronal tracing; Electron microscopy; Synapse; Immunohistochemistry

1) In order to determine non-descending nerve terminals in the intermediolateral nucleus (IML), rat spinal cord was transected at T6 level. Seven days after the operation, serotonin (5-HT)-immunoreactive terminals disappeared completely from the IML of lower spinal cord. However, substance P-, enkephalin-, and tyrosine hydroxylase (TH)-immunoreactive nerve terminals remained in the IML.Electron microscopic observations revealed presence of synaptic contacts between these immunoreactive terminals and IML neurons. This result indicates presence of neuronal inputs from neurons in intrinsic or sub-spinal structures to the sympathetic preganglionic neurons.2) To examine a possible origin of TH-immunoreactive non-descending terminals in the IML,retrograde neuronal tracing experiments were made. After injection of cholera toxin B (CTB) into the spinal cord of levels T2-13, postganglionic cells located in thoracic sympathetic ganglia (T2-11) were labeled preferentially with CTB.Injections into more rostral (C6) or caudal (L1, L5) spinal cord segments resulted in few labeled ganglion cells. This result suggest that postganglionic neurons in the thoracic sympathetic ganglion project to thoracic spinal cord.

1)在T6水平横断大鼠脊髓，以确定中间外侧核(IML)内的非降支神经末梢。术后7天，5-羟色胺(5-HT)免疫反应阳性终末从下段脊髓IML完全消失。然而，P物质、脑啡肽和酪氨酸羟化酶(TH)免疫反应神经末梢仍留在IML内。电子显微镜观察显示，这些免疫反应终末与IML神经元之间存在突触接触。这一结果表明存在从固有结构或脊髓下结构的神经元到交感节前神经元的神经元输入。2)为了研究IML内TH免疫反应阳性非降支终末的可能来源，进行了神经元逆行追踪实验。将霍乱毒素B(CTB)注入脊髓T2-13节段后，位于胸交感神经节(T2-11)的节后细胞优先被CTB标记。向更多的头端(C6)或尾端(L1、L5)脊髓节段注入霍乱毒素B后，标记的节细胞较少。这一结果提示，胸交感神经节内的节后神经元投射到胸髓。

项目成果

Fujiyama,F.and Masuko,S: "Association of dopaminergic terminals and neurous releasing nitric oxide in the rat striatum : aneectron microscopic study using NADPH-diaphorase histochemistry and tyrosine hydroxylase immunohistochemistry." Brain Res.Bulletin.

Fujiyama, F. 和 Masuko, S：“大鼠纹状体中多巴胺能末端与神经释放一氧化氮的关联：使用 NADPH 心肌黄酶组织化学和酪氨酸羟化酶免疫组织化学进行的电子显微研究。”