权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Cytopathological studies of Ewing's sarcoma and an application of pathologic diagnosis using newly produced monoclonal antibodies

尤文氏肉瘤的细胞病理学研究及新制备的单克隆抗体在病理诊断中的应用

基本信息

批准号：
01570169
负责人：
NOJIMA Takayuki
金额：
$ 1.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

1. Three human Ewing's sarcoma lines were established, and these were investigated for their morphological and cytogenetic characterization and molecular analysis.2. Chromosome analysis revealed a translocation, t(11 ; 22)(q24 ; q12)in two of three lines, as reported previously for Ewing's sarcoma and primitive neuroectodermal tumors of bones and soft tissues.3. Morphology of cell cultures was unaffected by exposure to dbcAMP. Values of neuron-specific enolase were high in cultured cells, supematant of the culture fluid, and nude mice serum.4. Two clones of monoclonal anti-Seki antibodies were produced using the classical hybridoma technology. Expression of these target antigens was found in tumor cells of Ewing's sarcoma, neuroblastoma and osteosarcoma among tumor cells of Ewing's sarcoma, neuroblastoma, osteosarcoma rhabdomyosarcoma, malignant lymphoma and other carcinomas examined.5. The findings of Southem blot and Northern blot analyses of the N-myc, C-myc, Nras, ets-1 and PDGFB oncogenes were not specific for Ewing's sarcoma.6. We compared Ewing's sarcoma with rbabdomyosarcoma and small cell osteosarcoma by morphological, immunobistochemical and cytogenetic studies. The morphological features of neuroblastoma is easily confused with Ewing's sarcoma by light microscopy and immunohistochemistry. We established a new neuroblastoma cell line(HNB- 18)and investigated a difference between neuroblastoma and Ewing's sarcoma. These results described above(1-6)suggest that Ewing's sarcoma may originate in a primitive neuroectodermal cell. Although a difference between Ewing's sarcoma and primitive neuroectodermal tumor is still unclear from our work, we consider that these two tumors may be expressed by the histological grade of differentiation.

1.建立了3株人尤文肉瘤细胞系，并对其进行了形态学、细胞遗传学和分子生物学研究.染色体分析显示三个品系中有两个易位t（11 ; 22）（q24 ; q12），与以前报道的尤文肉瘤和骨及软组织原始神经外胚层肿瘤一样。细胞培养物的形态不受暴露于dbcAMP的影响。神经元特异性烯醇化酶在培养细胞、培养液上清液和裸鼠血清中均较高.使用经典的杂交瘤技术产生两个单克隆抗Seki抗体的克隆。在尤文肉瘤、神经母细胞瘤、骨肉瘤、横纹肌肉瘤、恶性淋巴瘤等肿瘤细胞中，尤文肉瘤、神经母细胞瘤和骨肉瘤的肿瘤细胞均表达这些靶抗原. N-myc、C-myc、Nras、ets-1和PDGFB癌基因的Southem blot和北方blot分析结果对尤文肉瘤无特异性.我们从形态学、免疫组化和细胞遗传学三个方面对尤文肉瘤与横纹肌肉瘤和小细胞骨肉瘤进行了比较。神经母细胞瘤的形态学特征在光镜及免疫组化上易与尤文肉瘤混淆。我们建立了一个新的神经母细胞瘤细胞系（HNB- 18），并研究了神经母细胞瘤和尤文肉瘤之间的差异。上述结果（1-6）提示尤文肉瘤可能起源于原始神经外胚层细胞。虽然尤文氏肉瘤和原始神经外胚层肿瘤之间的差异仍不清楚，从我们的工作，我们认为，这两种肿瘤可能是由分化的组织学分级表示。