Analysis of the Pathogenic Mechanism in Schistosomiasis-Functional Decline of the Mitochondria in Parenchymal Liver Cells

A Mitochondria-Rich Fracion isolated from ICR和C3 H女性老鼠的livers 8至10周后，Schistosoma Mansoni感染显示，以成为Succccccinate的不平衡状态-支持的反应。A moderate to strong mitochon？al Mg^\+\-dependent ATPase activity at neutral pH was demonstrated cytochmecally in the parenchymal liver cells of S. mansoni-infected mice。Moreover，Malate脱氢酶、NADH脱氢酶、细胞色素氧化酶和谷氨酸脱氢酶的酶活性也明显地减少了来自受感染老鼠的大鼠中的富含甲酮的片段。Furthermore，对受感染的老鼠的liver的超结构观测，清楚地演示了一些形态学变化的Hepatic Mitochondria， I。e.，晶体的流失、晶体的流失和矩阵的电子密度的分化;在未感染的、正常的、治疗中，Hepatic Mitochondria也被证明是在未感染的、正常的老鼠中。 ... More 15，000克x30分钟的裂解剂的戊类注射液的超自然剂（egg-granulomas）。However，在S.C.中，在对Hepatic Mitochondria的反应中，存在着不值得称赞的变化。曼森成年蠕虫。该活性物质部分地从硫酸铵分段、Sephadex G-200凝胶过滤和DEAE-Sephadex A-25柱色谱法中提取的egg-granulomas。Intraperitoneal injection of the partially purified substance into unifected， normal mice resulted in the decline of the activities of several enzymes in Hepatic metochondria and in the ultrastructural changes in the mitochondria of parencymal liver cells。The same biochemical changes were also observed in the primary cultured hepatocytes treated with the partially purified substance.These findings suggest that the egg-granulomas produce some substance（s） which may be responsible for reduction of the activities of respiration and several enzymes localized in the mitochondria of the parenchymal liver cells in S. mansoni-infected mice。Less（低）

A mitochondria-rich fracion isolated from the livers of ICR and C3H female mice 8 to 10 weeks after Schistosoma mansoni infection showed to be uncoupling state of the succcinateーsupported respiration. A moderate to strong mitochondrial Mg^<++>-dependent ATPase activity at neutral pH was demonstrated cytochmecally in the parenchymal liver cells of S. mansoni-infected mice. Moreover, the enzymatic activities of malate dehydrogenase, NADH dehydrogenase, cytochrome oxidase and glutamate dehydrogenase also decreased significantly in the mitochondria-rich fraction from the livers of infected mice. Furthermore, an ultrastructural observation of the liver of infected mice clearly demonstrated some morphological changes of hepatic mitochondria, i. e., swelling, disappearance of cristae and deciline of electron density of matrix.The uncoupling state of the respiration and the ultrastructural changes of the hepatic mitochondria were also demonstrated in the uninfected, normal mice treated with in … More traperitoneal injection of the dialysate of 15,000g x 30 min supernatant of crude extracts of egg-granulomas. However, there was no appreciable change in the respiration of hepatic mitochondria in the mice treated with culture fluid or crude extract of S. mansoni adult worms. The active substance was partially purified from crude extract of egg-granulomas by ammonium sulfate fractionation, Sephadex G-200 gel filtration and DEAEーsephadex A-25 column chromatography. Intraperitoneal injection of the partially purified substance into unifected, normal mice resulted in the decline of the activities of several enzymes in hepatic metochondria and in the ultrastructural changes in the mitochondria of parencymal liver cells. The same biochemical changes were also observed in the primary cultured hepatocytes treated with the partially purified substance.These findings suggest that the egg-granulomas produce some substance(s) which may be responsible for reduction of the activities of respiration and several enzymes localized in the mitochondria of the parenchymal liver cells in S. mansoni-infected mice. Less