Study on apoprotein of the convulsion-inducing factor in schistosome egg granulomas

血吸虫虫卵肉芽肿惊厥因子脱辅基蛋白的研究

• 批准号: 11670249
• 负责人: TANABE Masanobu
• 金额: $ 1.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670249/
• 关键词: Schistosoma mansoni; Egg granuloma; Convulsion-inducing factor; Monoclonal antibody; Lipoprotein; Apoprotein

A convulsion-inducing lipoprotein (CILIP) was purified from hepatic egg granulomas and spleens of mice infected with Schistosoma mansoni, and its physical and chemical properties were characterized. The CILIP-like convulsion-inducing substances could be also purified from various tissues of uninfected and infected mice with S.mansoni, several inbred strains and immunocompromised mice cared under SPF condition, rat, rabbit, and human according to the method developed for CILIP purification. These substances showed the same physical and chemical properties as CILIP.Intravenous injection of these substances caused the same sign and symptoms as have been demonstrated by CILIP injection. Moreover, in vivo study indicates that these substances may act through the same mechanism as CILIP.These substances are, therefore, considered to be compatible lipoproteins with CILIP.It is, therefore, likely that CILIP may be widely distributed in various animal and human tissues.Rabbit and mouse polyclonal antibody to CILIP completely inhibited not only CILIP activity but also the activities of convulsion-inducing substances isolated from various animal and human tissues. This evidence suggests that there are a common molecule among these convulsion-inducing lipoproteins. The existence of two types of common molecules in the convulsion-inducing lipoproteins isolated from various animal and human tissues was confirmed by two dimensional electrophoresis. All of these evidences suggest that two types of common peptides probably play an essential role in the pharmacological action of CILIP.

从曼氏血吸虫感染小鼠的肝虫卵肉芽肿和脾脏中分离纯化了一种惊厥诱导脂蛋白（CILIP），并对其理化性质进行了研究。根据为CILIP纯化开发的方法，还可以从未感染和感染曼氏血吸虫的小鼠、几种近交系和在SPF条件下护理的免疫受损小鼠、大鼠、兔和人的各种组织中纯化CILIP样惊厥诱导物质。这些物质显示出与CILIP相同的物理和化学性质。静脉注射这些物质引起的体征和症状与CILIP注射液相同。此外，体内研究表明，这些物质可能通过与CILIP相同的机制起作用。因此，这些物质被认为是与CILIP相容的脂蛋白。因此，CILIP可能广泛存在于动物和人体的多种组织中，抗CILIP的兔、鼠多克隆抗体不仅能完全抑制CILIP的活性，而且能抑制惊厥的活性。从各种动物和人体组织中分离的诱导物质。这一证据表明，在这些惊厥诱导脂蛋白中有一个共同的分子。从动物和人体组织中分离的致惊厥脂蛋白经双向电泳证实存在两种类型的共同分子。这些证据表明，两种类型的共同肽可能在CILIP的药理作用中发挥重要作用。