Structural Analysis of Flagella of Vibrio

弧菌鞭毛的结构分析

• 批准号: 01570238
• 负责人: SHINODA Sumio
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570238/
• 关键词: Vibrio; Flagella; Structure gene; Serological taxonomy; Polar monotrichous flagella; Lateral flagella; Flagellin; モノクロ-ナル抗体; 血清学的分類

Some vibrio species have two kinds of flagella, polar Monotrichous (M) and Lateral (L), on a cell. Southern blot hybridization with flagellar gene of Vibrio Pardhaemolyticus as the probe showed DNA homology of M-flagellar gene in all species of vebrios and L-flagellar gene in all L-flagella-having vibrios, whereas the hybridization L-flagellar probe with vibrios having only M-flagella was not observed, suggesting absence of L-flagellar gene in these vibrios. Two different-sized flagellin molecules, 42 kd and 44 kd, were found in M-flagella of all vibrios species tested. Gel diffusion test with polyclonal antibody showed immunological homogeneity of these two flagellins. However, result obtained by monoclonal antibodies suggested existence of epitopes which exist only in one of the two flagellins. Similar N-terminal amino acid sequences were found in the two M-flagellins of same species. However, similarity of the sequence between L- and M-flagellin of same species was not so high, approximately 30 to 40 %. This level was almost same with the similarity with the sequence of enterobacteria such as Salmonella Escherichia coli. The genes of L- and M- flagella seemed to evolve independently, although their ancestor be common. Endentry, although their ancestor should be common.

有些弧菌在一个细胞上有两种鞭毛，极单鞭毛(M)和侧鞭毛(L)。以副溶血性弧菌的鞭毛基因为探针的Southern杂交结果显示，所有弧菌的M鞭毛基因和L鞭毛都有同源性，而L鞭毛探针与弧菌只有M鞭毛的杂交没有观察到L鞭毛基因的存在，提示这些弧菌中不存在L鞭毛基因。在所有供试弧菌的M鞭毛中都发现了两种不同大小的鞭毛蛋白分子，分别为42kd和44kd。多克隆抗体凝胶扩散试验表明，这两种鞭毛具有免疫均一性。然而，通过单抗获得的结果表明，仅存在于两种鞭毛中的一种中存在表位。在同一物种的两个M-鞭毛中发现了相似的N-末端氨基酸序列。而同种的L鞭毛蛋白与M鞭毛蛋白的序列相似性并不高，约为30%~40%。这一水平与沙门氏菌、大肠杆菌等肠道细菌的序列基本一致。L鞭毛和M鞭毛的基因似乎是独立进化的，尽管它们的祖先是共同的。Endentry，尽管它们的祖先应该是共同的。

项目成果

Chowdhury, M. A. R., Yamanaka, H., Miyoshe, S. and Shinoda, S.: "Ecology of seasonal distribution of Vibrio parahaemolytics in aquatic environment of temperate region." FEMS Microbiol. Ecol.74. 1-10 (1990)

Chowdhury, M. A. R.、Yamanaka, H.、Miyoshe, S. 和 Shinoda, S.：“温带地区水生环境中副溶血弧菌季节性分布的生态学”。

安井 哲史: "ビブリオ属菌鞭毛の構造遺伝子と抗原性" 日本細菌学雑誌. 45. 468- (1990)

Satoshi Yasui：“弧菌鞭毛的结构基因和抗原性”日本细菌学杂志 45. 468- (1990)。

安井哲史,篠田純男: "ビブリオ属菌の鞭毛遺伝子と抗原性" 日本細菌学雑誌. 45. 468 (1990)

Satoshi Yasui，Sumio Shinoda：“弧菌的鞭毛基因和抗原性”日本细菌学杂志 45. 468 (1990)。

Chowdhury,M.A.R.: "Ecology of seasonal distribution of Vibrio parahaemolyticus in aquatic envronments of temperate region." FEMS Microbiol. Ecol.74. 1-10 (1990)

Chowdhury，M.A.R.：“温带地区水生环境中副溶血性弧菌季节性分布的生态学。”

Yasui, T. and Shinoda, S.: "Flagellar gene and antigencity of genus Vibrio." Jpn. J. Bacterol.45. 468 (1990)

Yasui, T. 和 Shinoda, S.：“弧菌属的鞭毛基因和抗原性。”