Structural Analysis of Flagella of Vibrio

有些弧菌在一个细胞上有两种鞭毛：极单鞭毛（M）和侧鞭毛（L）。以副溶血性弧菌鞭毛基因为探针的Southern杂交结果表明，所有弧菌的M鞭毛基因和所有含L鞭毛的弧菌的L鞭毛基因具有DNA同源性，而与仅含M鞭毛的弧菌杂交的L鞭毛探针则没有发现，表明这些弧菌中不存在L鞭毛基因。两种不同大小的鞭毛蛋白分子，42 kd和44 kd，发现在所有测试的弧菌的M-鞭毛。多克隆抗体的凝胶扩散试验表明，这两种鞭毛蛋白具有免疫同源性。然而，通过单克隆抗体获得的结果表明存在仅存在于两种鞭毛蛋白之一中的表位。在同一物种的两种M-鞭毛蛋白中发现了相似的N-末端氨基酸序列。然而，同一物种的L-和M-鞭毛蛋白之间的序列相似性不是很高，大约30 - 40%。这一水平与沙门氏菌、大肠杆菌等肠杆菌的序列相似性基本一致。L-鞭毛和M-鞭毛的基因似乎是独立进化的，尽管它们的祖先是共同的.虽然他们的祖先应该是共同的。

Some vibrio species have two kinds of flagella, polar Monotrichous (M) and Lateral (L), on a cell. Southern blot hybridization with flagellar gene of Vibrio Pardhaemolyticus as the probe showed DNA homology of M-flagellar gene in all species of vebrios and L-flagellar gene in all L-flagella-having vibrios, whereas the hybridization L-flagellar probe with vibrios having only M-flagella was not observed, suggesting absence of L-flagellar gene in these vibrios. Two different-sized flagellin molecules, 42 kd and 44 kd, were found in M-flagella of all vibrios species tested. Gel diffusion test with polyclonal antibody showed immunological homogeneity of these two flagellins. However, result obtained by monoclonal antibodies suggested existence of epitopes which exist only in one of the two flagellins. Similar N-terminal amino acid sequences were found in the two M-flagellins of same species. However, similarity of the sequence between L- and M-flagellin of same species was not so high, approximately 30 to 40 %. This level was almost same with the similarity with the sequence of enterobacteria such as Salmonella Escherichia coli. The genes of L- and M- flagella seemed to evolve independently, although their ancestor be common. Endentry, although their ancestor should be common.