Interaction of secreting pathogenic factors in vibriosis.

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Bacteria of genus Vibrio are habitant of natural aquatic environment and include some pathogenic species which secret various pathogenic factors. These factors usually cause symptoms by cooperating with or complementing to other factors. This research project was carried out to understand general mechanism of vibriosis by studying cooperative and complementary action of pathogenic factors of vibrios, such as hemolysin, protease, siderphore or enterotoxin of Vibrio vulnificus and V.mimicus.Hemolysisn and proteases were extensively studied in this research. Proteases of vibrios causing systemic infection, such as V.vulnificus, act directly as the toxic factor by stimulating vascular permeability. However proteases of enteropathogenic vibrios, such as V.mimicus, show indirect action ; influencing the colonization mechanism by modifying intestinal membrane, activation or inactivation of protein toxins by partial digestion of the peptide for nicking some special part.Although hemolytic action of V.mimicus hemolysin was not influenced by partial digestion with protease produced by the vibrio, enterotoxic activity of the hemolysin was lost by the proteolysis. V.vulnificus hemolysin was also nicked by partial digestion of the vibrio protease, and the influenc to the activity was different between the hemolysins of biotype 1 and 2. These results are useful information for understanding mechanism of vibrio infection.