Interaction of secreting pathogenic factors in vibriosis.

弧菌病中分泌致病因子的相互作用。

• 批准号: 10470069
• 负责人: SHINODA Sumio
• 金额: $ 6.21万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470069/
• 关键词: Pathogenic vibrio; Protease; Hemolysin; Vibrio vulnificus; Vibrio mimicus; タンパク毒素; ヘモリジン; エンテロトキシン

Bacteria of genus Vibrio are habitant of natural aquatic environment and include some pathogenic species which secret various pathogenic factors. These factors usually cause symptoms by cooperating with or complementing to other factors. This research project was carried out to understand general mechanism of vibriosis by studying cooperative and complementary action of pathogenic factors of vibrios, such as hemolysin, protease, siderphore or enterotoxin of Vibrio vulnificus and V.mimicus.Hemolysisn and proteases were extensively studied in this research. Proteases of vibrios causing systemic infection, such as V.vulnificus, act directly as the toxic factor by stimulating vascular permeability. However proteases of enteropathogenic vibrios, such as V.mimicus, show indirect action ; influencing the colonization mechanism by modifying intestinal membrane, activation or inactivation of protein toxins by partial digestion of the peptide for nicking some special part.Although hemolytic action of V.mimicus hemolysin was not influenced by partial digestion with protease produced by the vibrio, enterotoxic activity of the hemolysin was lost by the proteolysis. V.vulnificus hemolysin was also nicked by partial digestion of the vibrio protease, and the influenc to the activity was different between the hemolysins of biotype 1 and 2. These results are useful information for understanding mechanism of vibrio infection.

弧菌属细菌是自然水环境中的栖息地，含有多种致病因子。这些因素通常通过与其他因素协同或互补而导致症状。本研究通过研究创伤弧菌和拟态弧菌的溶血素、蛋白酶、铁载体或肠毒素等弧菌致病因子的协同互补作用，了解弧菌病的一般发病机制。引起全身感染的弧菌的蛋白酶，如创伤弧菌，通过刺激血管通透性直接作为毒性因子发挥作用。而拟态弧菌等肠源性弧菌的蛋白水解酶具有间接作用，通过改变肠膜来影响定植机制，通过部分消化蛋白毒素来激活或灭活蛋白毒素以切割某些特殊部分。虽然拟态弧菌溶血素的溶血作用不受弧菌产生的酶的部分消化的影响，但溶血素的肠毒素活性因蛋白降解而丧失。创伤弧菌溶血素也是通过部分消化弧菌蛋白酶而被切割出来的，并且生物型1和生物型2的溶血素对活性的影响是不同的。这些结果为了解弧菌感染的机制提供了有用的信息。

项目成果

Bi, K., et al.: "Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction"Microbiol. Immunol.. 44. 149-153 (2000)

Bi, K. 等人：“通过任意引发的聚合酶链式反应分析拟态弧菌临床菌株”Microbiol。

Shi, L., Miyoshi, S., Shinoda S.et al.: "Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction."J.Health Sci.. 46. 63-65 (2000)

Shi, L., Miyoshi, S., Shinoda S.等人：“通过聚合酶链式反应测定拟态弧菌分离株中溶血素基因（vmh、tdh 和 hlx）的存在。”J. Health Sci.. 46. 63

Miyoshi, S. et al.: "The hemagglutinating action of Vibrio vulnificus metalloprotease"Microbiol. Immunol.. 43. 79-82 (1999)

Miyoshi, S. 等人：“创伤弧菌金属蛋白酶的血凝作用”微生物学。

Miyoshi,S.: "The hemagglutinating action of Vibrio vulnificus metalloprotease." Microbiology and Immunology. 43. 79-82 (1999)

Miyoshi,S.：“创伤弧菌金属蛋白酶的血凝作用。”

Miyoshi,S.: "Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease,a member of the thermolysin family." Infection and Immunity. 66. 4851-4855 (1998)

Miyoshi,S.：“由嗜热菌蛋白酶家族成员之一的创伤弧菌金属蛋白酶引起的出血反应的特征。”