The effect afair pollutants on productibility of allergen specific antibody

污染物对过敏原特异性抗体生产能力的影响

• 批准号: 01570301
• 负责人: KAGAWA Jun
• 金额: $ 1.41万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570301/
• 关键词: Nitreogen di oxide; specific antibody; ovalbumin; ELISA; guinea pig; sensitization; オミレ-ション法; 感作; オシレ-ション法; 大気汚染物質; 抗原特異的IgE抗体; 気道反応性; 超音波オブライザ-

In order to investigate th effect of air pollutants on allrgic sensitization to inhaled antigen, 2.5ppm N02 exposure experiments using guinea pigs were conductd. 60 male guinea pigs divided into three groups were exposed to 25mg/m3 ovalbumin (OA) aerosol or 5mg/m3 OA or 5mg/m3 OA plus 2.5ppm N02 in a original OA exposure system with a 500L exposure chamber for 4 hrs/day, 5days/week, for 4 weeks. Every other day, before and immediate after 4hr-exposure measurement of respiratory flow resistance were performed with oscilation method. After 4 week exposure, specific antibodies against OA are measured in serum and bronchoalveolar fluid by a direct enzyme immunoassay. The first bronchial reaction during exposure were observed on the 8-10th day. Intensity of reaction depended on the concentration of OA and was independent of N02 exposure. The relationships between OA-specific antibodies in serum and bronchoalveolar fluid and the intensity of bronchial reaction are under present investigation.

为探讨空气污染物对吸入性抗原致敏作用的影响，用豚鼠进行了2.5ppm NO2暴露实验。将60只雄性豚鼠分成3组，分别暴露于25 mg/m~3的卵白蛋白气雾剂、5 mg/m~3的卵白蛋白或5 mg/m~3的卵白蛋白+2.5ppm的NO_2，在500L曝气室中暴露4小时/天，5天/周。每隔一天、暴露前和暴露后即刻用示波法测定呼吸流阻。暴露4周后，用直接酶免疫法检测血清和支气管肺泡液中抗OA的特异性抗体。染毒后第8~10天观察第一次支气管反应。反应强度依赖于OA的浓度，与NO2暴露无关。血清和支气管肺泡液中的OA特异性抗体与支气管反应强度之间的关系正在研究中。

项目成果

Satoh,T.,Kagaw,J.: "Ellects of 2.5ppm NO_2 exposure on allergic seusitzation" J.Alleagy clin.Inmunol.

Satoh,T.,Kagaw,J.：“选择 2.5ppm NO_2 暴露对过敏性惊厥的影响”J.Alleagy clin.Inmunol。

Satoh, T., et al.: "Effects of 2.5 ppm NO2 exposure on allergic sensitization in the guinea pig" J Allergy Clin Immunol.

Satoh, T., et al.：“2.5 ppm NO2 暴露对豚鼠过敏性致敏的影响”J Allergy Clin Immunol。

Satoh,T.,Kagawa,J.: "Timecourse of allergic reaction during coatiuuous allergeu exposure in the guiven pig" Jut.Arch.Allergy appl.Iumuunol.

Satoh,T.,Kakawa,J.：“幼仔猪长时间接触过敏原期间过敏反应的时间进程”Jut.Arch.Allergy appl.Iumuunol。

Satoh,T.,Kagawa,J.: "Effects of 2.5ppm No_2 exposure on allergic seusitization" J.Allergy Clin.Immunol.

Satoh,T.,Kakawa,J.：“2.5ppm No_2 暴露对过敏性惊厥的影响”J.Allergy Clin.Immunol。

Satoh,T.Kagawa,J.: "Timecourse of allergic reaction during continuous allergen exposure in the guine a pig" Int.Arch.Allergy Appl.Immunol.

Satoh,T.Kakawa,J.：“豚鼠连续过敏原暴露期间过敏反应的时间进程”Int.Arch.Allergy Appl.Immunol。