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Experimental Examination of Organic Toxicity for Trihalomethane.

三卤甲烷有机毒性的实验检验。

基本信息

批准号：
01570315
负责人：
HOBARA Tatsuya
金额：
$ 1.28万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

There are numerous surveys concerning TRI toxicity to animals and humans. In recent years, two carcinogenicity bioassays have indicated that the daily administration of high oral doses of TRI leads to an increased incidence of hepatocellular carcinoma in B6C3Fl mice.TRI pollution of surface water as well as ground water is increasing year by year, creating serious problem in many advanced nations. In contrast, relatively little is known about its absorption from the gastrointestinal tracts. In our previous paper, we administered TRI solutions at three concentrations to the three segments of intestinal tracts of the circulation loop system in dogs, and measured TRI and its metabolites in serum or blood, urine, bile and circulating solution However, the amounts of administered solution was so large (about 500 ml) that we could not clarify the detail of the small volume of TRI absorption from the intestinal tracts. From these reason, we administered TRI solutions at three concentrations (0.1, 0.25 and 0.5%, about 20ml each) to the three segments of intestinal tracts (jejunum, ileum and colon) of the closed loop system in dog. We measured TRI and its metabolites, F-TCE, TCA and Conj-TCE, in the serum or blood, urine, bile and remaining solutions. The fraction of TRI absorbed from the intestine were 85-90% of the administered volume of TRI two hours after administration in all groups, and all parts of intestine readily absorbed TRI. Moreover, there were no significant differences between results from the three parts of the intestine. The combined biliary and urinary excretion ratios of TRI and its metabolitesto the total amounts absorbed from intestine were very low (0.6-1.6%) two hours after administration in all groups.There were no significant differences between the Tween 80 added groups and the non-added groups in the 0.1% TRI administered group.

关于三碘甲烷对动物和人类的毒性有许多调查。近年来，两项致癌性生物测定表明，每天口服高剂量的TRI可导致B6 C3 F1小鼠肝癌的发病率增加。TRI对地表水和地下水的污染逐年增加，已成为许多发达国家的严重问题。相比之下，关于其从胃肠道的吸收知之甚少。在我们之前的论文中，我们将三种浓度的TRI溶液给予犬循环回路系统的三段肠道，并测量了血清或血液、尿液、胆汁和循环溶液中的TRI及其代谢物。然而，给药溶液的量太大（约500 ml），我们无法阐明TRI从肠道吸收的小体积细节。因此，我们将三种浓度（0.1%、0.25%和0.5%，各约20 ml）的TRI溶液分别注入狗闭合环路系统的三段肠道（空肠、回肠和结肠）。我们测量了血清或血液、尿液、胆汁和剩余溶液中的TRI及其代谢产物F-TCE、TCA和Conj-TCE。在所有组中，在给药后2小时，从肠道吸收的TRI分数为TRI给药体积的85-90%，并且肠道的所有部分都容易吸收TRI。此外，肠的三个部分的结果之间没有显著差异。给药后2 h，各组TRI及其代谢物的胆汁和尿液排泄量占肠吸收总量的比例均很低（0.6-1.6%），0.1% TRI组加Tween 80组与不加Tween 80组之间无显著性差异。