Evaluation of neural toxicity of organic solvents using primary culture neurons

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In this study, we investigated intracellular CaィイD12+ィエD1 homeostasis effected by organic solvents. Not only CaィイD12+ィエD1, but NaィイD1+ィエD1, KィイD1+ィエD1 play important roles in neural activity. We tried to evaluate the effect on physical function such as release of neurotransmitter measuring CaィイD12+ィエD1, NaィイD1+ィエD1, and KィイD1+ィエD1 movements.Organic solvents is hydrophobic, and they had been investigated focused on electrophysiologic activities, membrane voltage and cation concentration of target organs in relation to membrane lipids. The method we used enabled to evaluate intracellular environment, intracellular CaィイD12+ィエD1 storage organelles.We also investigated electrical activities in brain slices involved synaptic transmission composing neural network. We could evaluated the change of intracellular CaィイD12+ィエD1 change along with ion currents, and we were acknowledged which has an important role, intracellular CaィイD12+ィエD1 or extracellular CaィイD12+ィエD1.