Evaluation of neural toxicity of organic solvents using primary culture neurons

使用原代培养神经元评价有机溶剂的神经毒性

• 批准号: 10670316
• 负责人: HOBARA Tatsuya
• 金额: $ 1.79万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670316/
• 关键词: organic solvents; neuron; ion currents; intracellular CaィイD12+ィエD1; 細胞内Ca^<++>; トリクロロエチレン; 海馬初代培養細胞; マイクロマニピュレーター; 新生ラット

In this study, we investigated intracellular CaィイD12+ィエD1 homeostasis effected by organic solvents. Not only CaィイD12+ィエD1, but NaィイD1+ィエD1, KィイD1+ィエD1 play important roles in neural activity. We tried to evaluate the effect on physical function such as release of neurotransmitter measuring CaィイD12+ィエD1, NaィイD1+ィエD1, and KィイD1+ィエD1 movements.Organic solvents is hydrophobic, and they had been investigated focused on electrophysiologic activities, membrane voltage and cation concentration of target organs in relation to membrane lipids. The method we used enabled to evaluate intracellular environment, intracellular CaィイD12+ィエD1 storage organelles.We also investigated electrical activities in brain slices involved synaptic transmission composing neural network. We could evaluated the change of intracellular CaィイD12+ィエD1 change along with ion currents, and we were acknowledged which has an important role, intracellular CaィイD12+ィエD1 or extracellular CaィイD12+ィエD1.

在本研究中，我们研究了有机溶剂对细胞内Ca γ - D12+ γ - D1稳态的影响。在神经活动中起重要作用的不仅是Ca γ γ γ γ γ + γ γ γ + γ γ，还有Na γ γ + γ γ + γ γ。我们试图评估对身体功能的影响，如神经递质的释放，测量Ca γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ。有机溶剂是疏水的，主要研究对象是靶器官的电生理活性、膜电压和阳离子浓度与膜脂质的关系。我们使用的方法能够评估细胞内环境，细胞内Ca - D12+ c - D1存储细胞器。我们还研究了与突触传递组成神经网络有关的脑电活动。我们可以评价细胞内Ca γ γ - D12+ γ - D1的变化随离子电流的变化，并认识到细胞内Ca γ - D12+ γ - D1和细胞外Ca γ - D12+ γ - D1的作用。

项目成果

芳原達也 他: "臓器移植に関する日本人の意識構造(第2報)-共分散構造モデルによる死生観について-"民族衛生学雑誌. 64. 183-192 (1998)

吉原达也等：“日本人关于器官移植的意识结构（第二次报告）-基于协方差结构模型的生死观-”《民族卫生杂志》64。183-192（1998）。

Hobara, T. et. al: "Estimation of the lethaltoluene concentration from the accidental death of Painting workers"Industrial Health. 38. 5-13 (2000)

霍原，T.等。

T,Hobara et al: "Pollution due to Trichloroacetic Acide in Clams(Tapes japonica)in an Estuary Adjacent to Industrial Areas" Bull.Environ.Contam.Toxicol. 60. 74-80 (1998)

T,Hobara 等人：“工业区附近河口的蛤蜊（粳稻）中三氯乙酸造成的污染”Bull.Environ.Contam.Toxicol。

T,Hobara et al: "Zinc oxide enhancement of chemiluminescence in rat neutrophils" Toxicol Letters. 95. 137 (1998)

T，Hobara 等人：“氧化锌增强大鼠中性粒细胞化学发光”Toxicol Letters。

Hobara,T.et.al: "Estimation of the lethaltoluene concentration from the accidental death of Painting workers"Industrial Health. 38. 5-13 (2000)

Hobara，T.et.al：“油漆工人意外死亡造成的致死性甲苯浓度的估算”工业健康。