Studies on the abnormality of receptors of parietal cell

壁细胞受体异常的研究

• 批准号: 01570410
• 负责人: TANI Norio
• 金额: $ 1.41万
• 依托单位: Tokai Univesity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570410/
• 关键词: parietal cell; receptor; acid secretion; intracellular calcium ion; cyclic AMP; ultrastructural changes; intracellular canaliculi; radioreceptor assay; アドレナリンレセプタ-; 内視鏡的Congo red試験; microsomal fraction; radioreceptorassay; 酸分泌反応

Investigation about sensitivity (responsibility) of parietal cells was performed by mainly using isolated parietal cells of guinea pig. Results are as follows.1). It was confirmed that measurement of acid secreting function of isolated parietal cells by ^<14>C-aminopyrine accumulation method is useful for the evaluation of sensitivity of the cells.2). It was confirmed that measurement of intracellular calcium ion of isolated parietal cells is useful for the evaluation of sensitivity of the cells in case of stimulation with muscarinic agonists and gastrin.3). It was confirmed that observation of ultrastructural changes of parietal cells are useful for evaluation of sensitivity of the cells. Parietal cells showed remarkable dynamic ultrastructural changes. following stimulation by secretagogues. The difference of inhibitory mechanism between H_2-blocker and proton pump inhibitor was observed using this method.4). Affinity for parietal cells of pirenzepine, a muscarinic receptor antagonist, was observed by radioreceptor assay using microsomal fraction prepared from isolated parietal parietal cells and it was revealed that pirenzepine has a direct action on the muscarinic receptors of parietal cells.5). It was shown that parietal cells have adrenergic beta_2 receptors by ^<14>C-aminopyrine accumulation method and radioreceptor assay.6). The investigation of ultrastructural changes of parietal cells obtained by endoscopic biopsy from 6 patients with gastroduodenal diseases revealed that sensitivity of parietal cells may not be the cause of the locational variation of the speed of discoloration during endoscopic Congo red test.

以离体豚鼠壁细胞为主要材料，对壁细胞的敏感性（责任）进行了研究。结果如下：1)。证实用^<14> c -氨基吡啶积累法测定离体壁细胞的泌酸功能可用于评价细胞的敏感性2)。研究证实，测定离体壁细胞的胞内钙离子可用于评估细胞在毒蕈碱激动剂和胃泌素刺激下的敏感性。证实了观察壁细胞超微结构的变化对评价细胞的敏感性是有帮助的。壁细胞动态超微结构变化明显。在促分泌剂刺激下。用该方法观察h_2 -阻滞剂与质子泵抑制剂抑制机制的差异。利用分离的顶壁细胞制备微粒体片段，通过放射受体测定观察到吡伦齐平（一种毒蕈碱受体拮抗剂）对顶壁细胞的亲和力，发现吡伦齐平直接作用于顶壁细胞的毒蕈碱受体5)。^<14 b> c -氨基嘌呤积累法和放射受体测定表明，顶壁细胞具有肾上腺素能β 2受体。通过对6例胃十二指肠疾病患者胃镜活检所得壁细胞超微结构变化的研究，发现壁细胞的敏感性可能不是内镜下刚果红试验中变色速度发生位置变化的原因。

项目成果

Muto N,Tani N and Miwa T: "Changes in cytosolic free calcium concentration by histamine stimulation in isolated guinea-pig parietal cells." Biology and Medicine of Signal Transduction,Proceedings of 7th International Conference on cyclic nucleotides,calci

Muto N、Tani N 和 Miwa T：“组胺刺激离体豚鼠壁细胞中胞质游离钙浓度的变化。”

唐沢 博之,木村 典夫,谷 礼夫,原 雅文,武藤 信美,近藤 均,宮沢 正行,三輪 剛,渡辺 慶一: "プロントポンプインヒビタ-の壁細胞におよぼす影響についての形態学的検討" 胃分泌研究会誌. 23. 73-76 (1991)

Hiroyuki Karasawa、Norio Kimura、Reio Tani、Masafumi Hara、Nobumi Muto、Hitoshi Kondo、Masayuki Miyazawa、Tsuyoshi Miwa、Keiichi Watanabe：“前泵抑制剂对壁细胞影响的形态学研究”胃分泌研究杂志。 (1991)

谷 礼夫: "壁細胞のムスカリン受容体の研究" 消化性潰瘍ー臨床と基礎. 9. 40-48 (1990)

Reio Tani：“壁细胞毒蕈碱受体的研究”消化性溃疡 - 临床和基础研究 (1990)。

唐沢 博之,木村 典夫,谷 礼夫,原 雅文,武藤 信美,近藤 均,宮沢 正行,三輪 剛,渡辺 慶一: "プロトンポンプインヒビタ-の壁細胞におよぼす影響についての形態学的検討" 胃分泌研究会誌. 23. 73-76 (1991)

Hiroyuki Karasawa、Norio Kimura、Reio Tani、Masafumi Hara、Nobumi Muto、Hitoshi Kondo、Masayuki Miyazawa、Tsuyoshi Miwa、Keiichi Watanabe：“质子泵抑制剂对壁细胞影响的形态学研究”胃分泌研究期刊。 76 (1991)

Tani N,Watanabe Y,Mutoh N,Karasawa H,Hara M and Miwa T: "Effect of pirenzepine on aminopyrine uptake by isolated guinea pig parietal cells." Scandinavian J.of Gastroenterology. 24. 1159-1162 (1989)

Tani N、Watanabe Y、Mutoh N、Karasawa H、Hara M 和 Miwa T：“哌仑西平对分离的豚鼠壁细胞摄取氨基比林的影响”。