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Microdialysis法を用いた脳内神経伝達物質の日内リズム

使用微透析法测定脑神经递质的昼夜节律

基本信息

批准号：
01570602
负责人：
笠原嘉
金额：
$ 1.22万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至无数据
项目状态：
已结题

项目摘要

数年来取り組んだマイクロダイアリシス法を確立し、その方法論についての問題点を論文として発表した(Nakahara et al.in Biogenic Amines)。また本方法の応用として線条体と前頭葉におけるド-パミン系の遊離調節機構について比較し、従来の方法では明確化されなかった前頭葉におけるド-パミン系遊離の調節の可能性を発表した(Ozaki et al.in J of Neurochemistry)。ついで脳内自己刺激行動において側坐核ド-パミン系が関与しており、ド-パミン取り込み阻害剤を併用することによって顕在化させうることを発表した(Nakahara et al.in Brain Research, Nakahara et al.Neuroscience Letters)。上述したこれまでの実績を踏まえた上で、ラット視交叉上核のインド-ルアミンをマイクロダイアリシス法で測定を始めた。手術侵襲から回復した段階で、自由行動下に視交叉上核のセロトニンと5-ヒドロキシインド-ルアミン酢酸の基礎値の測定に成功し、アンフェタミンに対するインド-ルアミンの反応を確かめ、用量依存的にセロトニンがアンフェタミンによって遊離が行われることを実証しつつある。今後、マイクロダイアリシスの利点である行動のモニタ-を併用し、行動のリズムと視交叉上核のインド-ルアミンのリズムの関係について検討する予定である。また名古屋大学農学部のグル-プとも共同し、鳩の松果体でのマイクロダイアリシスを始めており、生理的状態で松果体のメラトニンを経時的に計測する手法を確立しつつある。今後、鳥類でのリズムを本方法論を用いて検討を加えたいと思っている。

Over the past few years, we have made sure that there is a lot of information about the establishment and methods of the organization, and the methods and methods. We have made a list of the information in the literature (Nakahara et al.in Biogenic Amines). In this method, you need to use the system to determine whether or not you are in a position to do so. In this way, you can use the method to make sure that you are in a position to make sure that you are in a position to make sure that you are not in the market. The system is based on the list of possibilities (Ozaki et al.in J of Neurochemistry) of the system. In order to stimulate your own activities, you can use your own stimuli. You can use your own stimuli to stimulate your own activities. You can use your own stimuli to stimulate your own stimulation activities. You can use your own stimuli to stimulate your own activities. You can use your own stimuli to stimulate your own activities. You can use your own stimuli to stimulate your own activities. You can use your own stimuli to help you with your own stimulation activities. You can use your own stimuli to help you with your own stimulation activities. You can use your own stimuli to help you with your own stimulation activities. You can use your own stimuli to make sure that you don't know what to do. You can use your own information to stop the damage. You need to make sure that you don't know what to do. You need to make sure that you don't know how to do this. You need to make sure that you don't know what to do (Nakahara et al.in Brain Research, Nakahara et al.Neuroscience Letters). The above information is required to determine the starting point of the measurement system. In the event of a technological attack, there is a high level of success in the measurement of success in the TV cross-over nuclear monitoring system, which is based on the determination of the success of the test, the performance of the device, the accuracy of the test, the accuracy and the accuracy of the system. From now on, we will make sure that we can make a profit in order to make a profit in order to make a profit in the future. In the Department of Agriculture of Nagoya University, the department of agriculture, the pineal gland, and the pineal gland. In the future, we will use this method to increase the accuracy of this method.