New Regulatory Mechanism of Liver Gluconeogenesis and its Abnormality.

由于糖异生对葡萄糖稳态起着关键作用，因此分析肝脏中糖异生的调控机制具有重要意义。我们的研究结果如下：1.分析了糖类再灌胃大鼠肝脏中2，6-二磷酸果糖(F2，6P_2)的含量。F2、6P_2水平取决于1)肝脏中2-激酶活性与F_2的比值、6-P_2磷酸酶活性及2)血清中胰岛素与胰升糖素的比值。幼年内脏脂肪变性(JVS)小鼠与全身性肉碱缺乏相关。JVS给予肉碱治疗可有效治疗低血糖。这一结果表明了脂肪酸代谢在糖异生中的重要性。

Because gluconeogenesis plays a key role on glucose homeostasis, it is important to analyse the regulatory mechanism of gluconeogenesis in liver. Our results were as follows :1. We analyzed the levels of fructose 2, 6-bisphosphate (F2, 6P_2) in liver of rats after refeeding of carbohydiates. F2, 6P_2 levels depend on 1) ratio of 2 kinase activity to F2, 6P_2 phosphatase activity in liver and 2) ratio of insulin level to glucagon level in serum.2. Juvenile visceral steatosis (jvs) mouse was associated with systemic carnitine deficiency. Administration of carnitine to jvs could cure hypoglycemia. This results show that the importance of fatty acids metabolism on gluconeogenesis.