New Regulatory Mechanism of Liver Gluconeogenesis and its Abnormality.

肝脏糖异生的新调控机制及其异常。

• 批准号: 01570639
• 负责人: KUWAJIMA Masamichi
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570639/
• 关键词: Gluconeogenesis; Etomoxir; Fructose 2, 6-Bisphosphate; carnitine; fatty acid oxidation; carnitine palmitoyltransferase; 脂肪酸酸化; Etomoxir; carnitine palmifoyltransferase; 肝グリコ-ゲン; フルクト-ス2,6ービスリン酸; インスリン; グルカゴン

Because gluconeogenesis plays a key role on glucose homeostasis, it is important to analyse the regulatory mechanism of gluconeogenesis in liver. Our results were as follows :1. We analyzed the levels of fructose 2, 6-bisphosphate (F2, 6P_2) in liver of rats after refeeding of carbohydiates. F2, 6P_2 levels depend on 1) ratio of 2 kinase activity to F2, 6P_2 phosphatase activity in liver and 2) ratio of insulin level to glucagon level in serum.2. Juvenile visceral steatosis (jvs) mouse was associated with systemic carnitine deficiency. Administration of carnitine to jvs could cure hypoglycemia. This results show that the importance of fatty acids metabolism on gluconeogenesis.

由于糖异生在葡萄糖稳态中发挥着关键作用，因此分析肝脏糖异生的调节机制具有重要意义。我们的结果如下： 1．我们分析了重新喂食碳水化合物后大鼠肝脏中果糖2, 6-二磷酸（F2, 6P_2）的水平。 F2、6P_2水平取决于1)肝脏中2激酶活性与F2、6P_2磷酸酶活性的比率和2)血清中胰岛素水平与胰高血糖素水平的比率。2．幼年内脏脂肪变性（jvs）小鼠与全身性肉碱缺乏有关。给合资企业注射肉碱可以治疗低血糖。这一结果表明脂肪酸代谢对糖异生的重要性。

项目成果

M.Kuwajima,N.Kono,M.Horiuchi,Y.Imamura,A.Ono,Y.Inui,S.Kawata,T.Koizumi,J.Hayakawa,I.Siheki,S.Tarui: "Animal model of syotemic carnitine deficiency:Analysis in C3HーHー2ﾟ strain of mouse associated with juvenile visceral steatosis." Biochem.Biophys.Res.Commu

M. Kuwajima、N. Kono、M. Horiuchi、Y. Imamura、A. Ono、Y. Inui、S. Kawata、T. Koizumi、J. Hayakawa、I. Siheki、S. Tarui：“对称肉碱的动物模型缺陷：与幼年内脏脂肪变性相关的 C3H－H－2ﾟ 品系小鼠分析。” Biochem.Biophys.Res.Commu

I.Mineo,N.Kono,Y.Yamada,N.Hara,H.Kiyokawa,T.Hamaguchi,M.Kawachi,T.Yamasaki,H.Nakajima,M.Kuwajima,S.Tarui: "Gluiose infusion abolishes the excessive ATP degradation in worlsing muocles of a patient with Mc Ardle's Disease." Musck Nerve. 13. 618-620 (1990)

I.Mineo,N.Kono,Y.Yamada,N.Hara,H.Kiyokawa,T.Hamaguchi,M.Kawachi,T.Yamasaki,H.Nakajima,M.Kuwajima,S.Tarui：“葡萄糖输注消除了过量的

H.Kiyokawa: "Hyperinsulinemia due to impaired insulin clearance associated with fasting hypoqlycemia and postprandial hyperglycemia:an analysis of a patient with antiinsuline receptor antibodies" J.Clin.Endocrinol.Metab.69. 616-621 (1989)

H.Kiyokawa：“与空腹低血糖和餐后高血糖相关的胰岛素清除受损导致的高胰岛素血症：抗胰岛素受体抗体患者的分析”J.Clin.Endocrinol.Metab.69。

H.Kiyokawa,N.Kono,T.Hamaguchi,M.Kawachi,K.Tajima,I.Mineo,Y.Yamada,T.Shimizu,M.Kuwajima S.Tarui: "Hyperinsulinemio due to impaired insulin clearance associated with fasting hypoglycemia and postprandial hyperglycemia:An analysis of a patient with antiinsul

H.Kiyokawa、N.Kono、T.Hamaguchi、M.Kawachi、K.Tajima、I.Mineo、Y.Yamada、T.Shimizu、M.Kuwajima S.Tarui：“与空腹低血糖相关的胰岛素清除受损导致高胰岛素血症

Y.Yamada,N.Kono,H.Nakajima,T.Shimizu,H.Kiyokawa,M.Kawachi,A.Ono,T.Nishimura,M.Kuwajima,S.Tarui: "Low glucoseー1,6・bisphosphate and high fructoseー2,6ーBisphosphate concentrations in muscles of patients with glycogenosis types VII and V." Biochem.Biophys.Res.

Y. Yamada、N. Kono、H. Nakajima、T. Shimizu、H. Kiyokawa、M. Kawachi、A. Ono、T. Nishimura、M. Kuwajima、S. Tarui：“低葡萄糖－1,6·二磷酸盐和VII 型和 V 型糖原增多症患者的肌肉中果糖 2,6-二磷酸浓度较高。”Biochem.Biophys.Res。