Intracellular signal transduction and phospholipid metabolism in platelets and neutrophils

血小板和中性粒细胞的细胞内信号转导和磷脂代谢

• 批准号: 01570680
• 负责人: TAKAYAMA Hiroshi
• 金额: $ 1.47万
• 依托单位: Kyoto University (1990-1991)福井医科大学 (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570680/
• 关键词: Signal transduction; Platelet; Phospholipid; Protein phosphorylation

Our new findings during this research support are as follows. 1. Activation of protein kinase C before stimulation of thrombin or A23187 enhances release of arachidonate from platelet membrane. 2. Indomethacin inhibits transfer of arachidonate into phosphatidyl-ethanolamine as well as arachidonate release. 3. A23187 or thrombin can stimulate protein-tyrosine phosphorylation of platelets. 4. Thrombin-induced protein-tyrosine phosphorylation is not dependent on GTP-binding protein, intra-and extra-cellular Ca^<2+>, binding of fibrinogen to GPllbllla, thromboxane A_2 formation and released ADP. 5. A23187 or thromboxane A_2 evokes protein-tyrosin phosphorylation of platelets through increase of intracellular Ca^<2+> via unknown mechanisms. 6. A23187-induced protein-tyrosine phosphorylation may be involved in platelet aggregation but not in secretion. 7. There may be synergistic stimulation of protein-tyrosine phosphorylation between intracellular Ca^<2+> and protein kinase C.

我们在这项研究支持期间的新发现如下。1.在凝血酶或A23187刺激前激活蛋白激酶C可增强花生四烯酸从血小板膜的释放。2.吲哚美辛抑制花生四烯酸转移到磷脂酰乙醇胺以及花生四烯酸释放。3. A23187或凝血酶可刺激血小板的蛋白酪氨酸磷酸化。4.凝血酶诱导的蛋白酪氨酸磷酸化不依赖于GTP结合蛋白、细胞内和细胞外Ca^（2+）、纤维蛋白原与GP Ⅲ b的结合、血栓素A_2的形成和ADP的释放。5. A23187或血栓素A_2通过增加细胞内Ca^<2+>引起血小板蛋白酪氨酸磷酸化，其机制尚不清楚。6. A23187诱导的蛋白酪氨酸磷酸化可能参与血小板聚集，但不参与分泌。7.细胞内Ca^<2+>和蛋白激酶C之间可能存在协同刺激蛋白酪氨酸磷酸化的作用。

项目成果

高山 博史: "Vascular Cells and Lipid・derived Biologically Active Substauces血管と脂質生理活性物質" 血管. 13. 69-75 (1990)

Hiroshi Takayama：“血管细胞和脂质衍生的生物活性物质”血管。 13. 69-75 (1990)

高山 博史: "Arachidonate Metabolism of Vascular Cell血管細胞とアラキドン酸代謝" 日本血栓止血学会誌. 2. 265-273 (1991)

Hiroshi Takayama：“血管细胞的花生四烯酸代谢和花生四烯酸代谢”日本血栓和止血杂志 2. 265-273 (1991)。

Takayama, H.: "Vascular cells and lipid-derived biologically active substances" Jpn. J. Circulation Res.13. 69-75 (1990)

Takayama, H.：“血管细胞和脂质衍生的生物活性物质”Jpn。

Takayama, H.: "Ionophore A23187-induced proteintyrosine phosphorylation of human platelets : possible synergism between Ca^<2+> mobilization and protein Kinase C activation" Biochem. Biophys. Res. Commun.174. 922-927 (1991)

Takayama，H.：“离子载体A23187诱导的人血小板蛋白酪氨酸磷酸化：Ca 2+ 动员和蛋白激酶C激活之间可能的协同作用”Biochem。

高山博史: "血管と脂質生理活性物質" 血管. (1990)

高山浩：“血管和脂质生理活性物质”血管（1990）。