Role of Medullasin in Granulocytes in Biophylaxis

髓质素在粒细胞生物预防中的作用

• 批准号: 01570697
• 负责人: AOKI Yousuke
• 金额: $ 1.34万
• 依托单位: The Institute of Public Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570697/
• 关键词: Medullasin; Granulocytes; Fish oil; Inhibitors of medullasin; Neutral protease; Biophylaxis

Effect of environmental factors on the activity of medullasin in qranulocytes was investigated. Addition of fat from pig on the diet of mice caused an increase of medullasin activity in qranulocytes, however, that from bovine brought about a decrease of it. Addition of fish oil containing high percent of eicosapentaic acid and docosahexanoic acid to the diet of mice increased the protease activity. Low molecular weight peptide inhibitors of medullasin were purified from human bone marrow cells. Two inhibitors were found. They were composed of 34 or 36 amino acids corresponding to the sequence -terminal of human hemoglobin beta chain. They inhibited medullasin activity reversibly and non-competitively with substrate.

研究了环境因素对粒细胞髓质素活性的影响。小鼠饲料中添加猪脂肪可引起粒细胞髓质素活性升高，而牛脂肪则使其活性降低。在小鼠的饲料中添加高百分含量的二十碳五烯酸和二十二碳六酸的鱼油，可提高该酶的活性。从人骨髓细胞中提纯了髓鞘蛋白的低分子量肽抑制物。发现了两种抑制剂。它们由34或36个氨基酸组成，对应于人血红蛋白β链的序列末端。它们可逆地和底物非竞争性地抑制髓质素的活性。

项目成果

Kiyoshi Okano,Yosuke Aoki,Horohiko Shimizu,Masanobu Naruto: "Functional expression of human leukocyte elastase(HLE)/medullasin in eukaryotic cells" Biochem.Biophys.Res.Commun.167. 1326-1331 (1990)

Kiyoshi Okano、Yosuke Aoki、Horohiko Shimizu、Masanobu Naruto：“真核细胞中人白细胞弹性蛋白酶 (HLE)/髓质素的功能表达”Biochem.Biophys.Res.Commun.167。

Yosuke Aoki: "Role of medullasin in granulocytes in biophylaxisーInhibitory mechanism of antiーinflammatory glucocorticoids on medullasin activity.In Intracellular Proteolysis" Japan Scientiffic Societies Press,Tokyo, 2 (1989)

Yosuke Aoki：“粒细胞中髓质素在生物预防中的作用 - 抗炎糖皮质激素对髓质素活性的抑制机制。细胞内蛋白水解”日本科学协会出版社，东京，2 (1989)

Yosuke Aoki,Hideko Kimura,Tomomi Hase,Tsuneo Shimazu,Tosiro Maruyama,Hirohiko Shimizu: "Low molecular weight paptide inhibitors of medullasin:Purification and structure." J.Enzyme Inhibition. 3. 279-287 (1990)

Yosuke Aoki，Hideko Kimura，Tomomi Hase，Tsuneo Shimazu，Tosiro Maruyama，Hirohiko Shimizu：“髓质素的低分子量肽抑制剂：纯化和结构。”

Kunimatsu K.,Ichimaru E.,Kato I,Sonda Y,Aoki Y.,Yamamoto K.: "Granulocyte medullasin levels in gingival cervicular fluid from chronic adult periodontitis patients and experimental gingivitis subjects" J.Periodont.Res.25. 352-357 (1990)

Kunimatsu K.、Ichimaru E.、Kato I、Sonda Y、Aoki Y.、Yamamoto K.：“慢性成人牙周炎患者和实验性牙龈炎受试者牙龈宫颈液中的粒细胞髓质素水平”J.Periodont.Res.25。

Atsushi Ikai,Masae Makashima,Yosuke Aoki: "Inhibition of inflammatory proteinase,medullasin,by α2ーmacroglobulin and ovomacroglobulin" Biochem.Biophys.Res.Commun.158. 831-836 (1989)

Atsushi Ikai、Masae Makashima、Yosuke Aoki：“α2-巨球蛋白和卵巨球蛋白对炎症蛋白酶、髓质素的抑制”Biochem.Biophys.Res.Commun.158（1989）。