Interstitial Reactions in the Progress of Gastric Carcinoma

胃癌进展中的间质反应

• 批准号: 01570770
• 负责人: NAKATA Eiji
• 金额: $ 1.41万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570770/
• 关键词: cell cycle; gastric carcunoma; collagen; collagenase; GAG; 胃癌細胞周期

To clarify the mechanism of the development of gastric carcinona, NKN-28 and KATO-III gastric carcinoma cells of different histological types were grown in synchronous cultures, and the differences in collagen metabolism and GAG (glycosaminoglycans) production were investigated in relation to the cell cycle. The effects of conditioned medium on the collagen metabolism of fibroblasts at various phases of the gastric carcinoma cell cycle were also studied.1) MKN-28, KATO-III and fibroblasts all produced collagen and collagenase and showed high values in the losarithaic growthphase.2) Good synchronization was obtained with both the MKN-28 and KATO-III cells, 87.0% and 74.6%, respectively.3) Collagen metabolism of gastric carcinoma cells was significantly promoted in the S phase (P<0.01) of the cell cycle regardless of the histological type.4) The collagen synthesizing capacity of both poorly and well differentiated types of gastric carcinoma cells was compared at various cell cycles, and … More no significant differences were found.5) Since collagenase activity in the S phase of KATO-III cells of scirrhus gastric carcinoma origin was significantly higher than that in the S phase of 9KN-28 cells of well-differentiated tubular adenocarcinoma origin, it appeared that collagenase is closely related to the type of carcinoma development and that since it causes collagen decomposition it is deeply involved in extensive cancer cell infiltrative growth, one of factors in the mechanism of formation of scirrhus gastric carcinoma.6) Because the conditioned medium of the KATO-III in the S phase promoted collagen synthesis by fibroblasts, it appeared that humoral factors released from carcinoma cells play an important role in the fibroplasia of scirrhus gastric carcinoma.7) GAG production was promoted in the S phase of cell cycle regardless of histological type. Its main component was hyaluronic acid (HA), and HA production in the S phase of KATO-III was higher than that in the S phase of NKN-28. Since accompanying carcinoma cell infiltration and proliferation HA increases and inhibits collagen fibrosis, it is surmised that this facilitates the progress of the carcinoma. Less

为阐明胃癌发生发展的机制，将不同组织学类型的NKN-28和KATO-Ⅲ胃癌细胞同步培养，研究胶原代谢和糖胺聚糖（glycosaminoglycans，GAG）产生与细胞周期的关系。结果表明：（1）MKN-28、KATO-Ⅲ和成纤维细胞均能产生胶原和胶原酶，并在Losarithic生长期表现出较高的活性;（2）MKN-28和KATO-Ⅲ细胞具有良好的同步性，分别为87.0%和74.6%; 3）无论何种组织学类型的胃癌细胞，其胶原代谢均在细胞周期的S期显著增加（P<0.01）。4）比较了不同细胞周期的低分化型和高分化型胃癌细胞的胶原合成能力， ...更多信息 5）由于硬癌来源的KATO-III细胞S期胶原酶活性显著高于高分化管状腺癌来源的9 KN-28细胞S期胶原酶活性，胶原酶似乎与癌症发展类型密切相关，并且由于它导致胶原分解，因此它深深参与了广泛的癌细胞浸润生长，形成硬癌的机制中的因素之一。6）由于S期的KATO-III的条件培养基促进成纤维细胞的胶原合成，因此似乎癌细胞释放的体液因子在硬癌的纤维形成中起重要作用。7）GAG的产生在细胞周期的S期被促进，而与组织学类型无关。其主要成分是透明质酸（HA），并且KATO-III的S期HA产量高于NKN-28的S期。由于伴随着癌细胞浸润和增殖，HA增加并抑制胶原纤维化，推测这促进了癌的进展。少

项目成果

中田 英二: "間質コラ-ゲンとコラゲナ-ゼー胃癌間質反応に関する検討ー" 肝胆膵. 23. 625-632 (1991)

Eiji Nakata：“间质胶原和胶原酶对胃癌的反应的研究。”23. 625-632 (1991)

中田 英二: "胃癌の進展と間質反応に関する研究ー胃癌細胞周期からみたコラ-ゲン代謝の検討ー" 大阪医科大学雑誌. 48. 30-43 (1989)

Eiji Nakata：“胃癌进展和间质反应的研究 - 从胃癌细胞周期的角度检查胶原代谢”大阪医科大学杂志 48. 30-43 (1989)。

中田 英二: "胃癌の発育・進展とコラ-ゲン代謝" BIOTHERAPY. 5. 1441-1448 (1991)

Eiji Nakata：“胃癌的生长和进展以及胶原代谢”BIOTHERAPY。5. 1441-1448 (1991)。