Oncogene Expression and Analysis of DNA Ploidy Patterns in Brain Tumors

本课题的研究结果在1989 ~ 1990年的27次日本和国际医学会议上发表了14篇论文。研究内容和结果：1）流式细胞术分析脑肿瘤的细胞动力学流式细胞术（FCM）已成为研究各种脑肿瘤生物学特性的有效手段。使用这种仪器，我们可以阐明培养的脑肿瘤的特点：i）恶性肿瘤不一定表现出高增殖活性，ii）在良性肿瘤中，脑膜瘤表现出高增殖指数，易于培养。我们还提出了一种新的DNA倍性模式分类，2）FCM法研究抗癌药物的作用机制用DNA/BrdU双染法研究抗癌药物的作用机制，阐明顺铂和卡铂的作用机制流式细胞术检测脑肿瘤患者的免疫功能及NK细胞的抗肿瘤作用应用流式细胞术从T细胞亚群角度评价脑肿瘤患者的免疫功能。利用流式细胞仪的无菌分选功能从淋巴细胞中获得的NK细胞在体外具有抗肿瘤作用。4）流式细胞术检测脑肿瘤中癌基因的表达利用已建立的脑肿瘤细胞系，通过免疫染色和流式细胞术证实c-myc癌基因在体外的表达。对手术切除的各种脑肿瘤进行培养，并在这些细胞中鉴定出c-myc阳性细胞。其中，胶质瘤显示c-myc阳性与恶性程度相关。因此，建议这种方法的潜在临床应用。

The results of the present study subject were reported in 14 papers, published as a book, and presented at 27 Japanese and international medical meetings between 1989 and 1990.Studies made and the results :1) Floweytometric analysis of cell kinetics of brain tumorsFlow cytometry (FCM) has become an effective means for the study of biological features of various brain tumors. Using this instrumentation, we could clarify the characteristics of cultured brain tumors : i) malignant tumors did not necessarily show high proliferative activity, ii) among benign tumors, meningioma showed a high proliferative index and was easy to cultivate. We also proposed a new classification of DNA ploidy patterns, by which the correlation with malignancy could be evaluated.2) Investigation of action mechanism of antineoplastic agents using FCMAction mechanism of antineoplastic agents was investigated using the DNA/BrdU double staining method and the action mechanism of cisplatin and calboplatin was clarified.3) Flowcytometric assessments of immunopotentiality of patients with brain tumor and the antitumoral effects of NK cellsFCM was applied to evaluation of immunopotentiality of brain tumor patients from the T cell subsets. NK cells, which were obtained from lymphocytes by utilizing sterile sorting function of a flow cytometer, had antitumoral effects in vitro. Thus obtained NK cells were considered clinically applicable.4) Floweytometric examination of oncogene expressions in brain tumorsExpression of c-myc oncogene was confirmed in vitro using established cell lines of brain tumors by immunostaining and FCM. Various kinds of brain tumors taken at surgery were cultivated and c-myc positve cells were identified in these cells. Of these, glioma showed a correlation between c-myc positivity and malignancy. Thus, potential clinical use of this method is suggested.