The study on the accumuration of hematoporphyrin in rheumatoid synoviam and mechanism of destruction of synovial cell by the exposure of Argon dye laser.

类风湿滑膜中血卟啉的积累及氩染料激光照射破坏滑膜细胞机制的研究。

• 批准号: 01570852
• 负责人: INOUE Kazuhiko
• 金额: $ 0.51万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570852/
• 关键词: hematoporphyrin; Argon dye laser; rheumatoid arthritis; synovial cell; superoxide; arthoscopy; 関節鏡; アジュバント関節炎; 滑膜炎; アルゴンダイレ-ザ-; 滑膜切除

The hematoporphyrin accumurate in inflemmatory cell of ajuvant arthritis of rat in large amount and long period. After 48 hours of ajuvant injection, the hematoporphyrin accumurate more than after 24 and 72 hours of ajuvant injection. By the exposure of Argon dye laser, ajuvant arthritis showed the strong fluorescence according to existence of hematoporphyrin.In the tissue culture of synovium which was take in the arthroscopy, synovium was destroyed by the hematoporphyrin in the medium and exposure of Argon dye laser to the synovial cultureAfter the exposure of laser, synovial tissue proliferates, however, the character of synovium changed. The observation of the fluoresce of hematoporphyrin of synovial culture was performed by the exposure of Argon dye laser to know where the hematoporphyrin accumurate inside or outside of synovial cell. However, we were able to know the hematoporphyrin accumurated portion of synovial cell By the immuno chemical study, using tissuer culture of synovium the release of interleukin 1 was decreased by the hematoporhyrin in the medium and the exposure of Argon dye laser more than without the exposure of Argon dye laser. In the same culture, Superoxide was measured by the method of cytochrome C. The superoxide measured high value in the medium.

血卟啉在大鼠佐剂性关节炎炎症细胞中大量、长时间积累。给药48 h后血卟啉的积累量明显高于给药24 h和72 h后。在氩染料激光照射下，由于血卟啉的存在，佐剂关节炎表现出强烈的荧光。在关节镜下进行的滑膜组织培养中，介质中的血卟啉和氩气染料激光照射对滑膜造成破坏，激光照射后滑膜组织增生，但滑膜特性发生改变。采用氩染料激光照射，观察滑膜培养血卟啉的荧光，了解血卟啉在滑膜细胞内外的聚集位置。然而，通过免疫化学研究，我们能够知道滑膜细胞中血卟啉的蓄积部分，使用滑膜组织培养，介质中的血卟啉和氩气染料激光照射比没有氩气染料激光照射更能减少白细胞介素1的释放。在同一培养基中，用细胞色素c法测定超氧化物，超氧化物在培养基中测得高值。

项目成果

Kazuhiko Inoue: "Basults and Future of Arthroscopic Synovectomy for Rheumatoid Arthritis" Postgraduate course on surgical aspects in Rheumatic diseases (Jakarta Indonesia). (1990)

Kazuhiko Inoue：“类风湿性关节炎关节镜滑膜切除术的基础和未来”风湿性疾病外科方面的研究生课程（印度尼西亚雅加达）。

井上 和彦: "アジュバント関節炎への低出力YAGレ-ザ-照射の効果" 第34回日本リウマチ学会総会抄録集. 274 (1990)

Kazuhiko Inoue：“低功率 YAG 激光照射对佐剂性关节炎的影响”日本风湿病学会第 34 届年会记录 274（1990）。

Kazuhiko Inoue: "Effects of low power YAG laser for Ajuvant Arthritis of Rat" The second world congress international society for low power laser applications in medicine (Tokyo). (1990)

Kazuhiko Inoue：“低功率 YAG 激光对大鼠佐剂关节炎的影响”国际低功率激光医学应用学会第二届世界大会（东京）。

Kazuhiko Inoue: "Effects of Low Power YAG Laser for Ajuvant Arthritis of Rat" 学会発表 The Second World Congress International Society for Low Power Laser Applicatoins in Medicine(Tokyo). (1990)

Kazuhiko Inoue：“低功率 YAG 激光对大鼠佐剂性关节炎的影响”会议演讲，国际低功率激光医学应用学会第二届世界大会（东京）（1990 年）。

Kazuhiko Inoue: "Effects of YAG LASER Exposure in the Treatment of Rheumatiod Arthritis" XVII Congressoilar Dereumatologia. 109 (1989)

Kazuhiko Inoue：“YAG 激光照射治疗风湿性关节炎的效果”第十七届国会 Dereumatologia。