Detoction of Anti-Tumor-Promoters by Soft Agar Colony Forming Assay and Application to Inhibition of Urinary Bladder Carcinogenesis

早些时候，我们报告说，在软琼脂上形成了殖民地，用老鼠黑细胞展示了在大鼠治疗BBN中的纸张发育。我们提供了一种短期筛选方法，用于检测新的肉碱、生长因子和反肿瘤生长因子。我们也评估了NDG是一种被抑制的Saccharin-inducuced Colony形成，认为它在大鼠叶片致癌性中具有抗肿瘤促进活动。我们研究了一个长期的实验，研究了由BBN诱导的NDGA对低血细胞的致癌作用。Six-wk-old Male Fischer 344只老鼠分布在8个小组中。在4周内饮用0.05%BBN（5-8组）或不饮用0.05%BBN，然后饮用36周，他们饮用5%Sodium Saccharin （第2组和第6组）。所有幸存的动物都在第40周被杀。历史上的不正常现象是被调查的。第6组（87%）高于第7组（55%）的事件和数字的前碳水化合物。第1组，第2组，第3组和第4组没有显示任何不正常现象。这些结果证实，NDGA对大鼠体内的血液生成产生了抗肿瘤-推效应。

Previously, we reported that colony formation in soft agar was shown with rat bladder cells prior to papilloma development in rat treated BBN. And we provided a short-term screening method for detecting new carcinogens, promoters and antiーtumor-promoters. We also evaluated NDG A inhibited saccharin-induced colony formation, suggesting that it has anti-tumor-promoting activity in rat bladder carcinogenesis. We studied long-term experiment on the anti-tumor-promoting effect of NDGA on urinary bladder carcinogenesis in rats induced by BBN. Six-wk-old male Fischer 344 rats were distributed among 8 groups. The were given drinking with (groups 5-8) or without (groups 1-4) 0.05% BBN for 4weeks, and then for 36weeks, they were given powdered diet containing 5% Sodium Saccharin (groups 2and 6). All surviving animals were killed in week 40. Histological abnormalities were investigated. The incidence and numbers of precarcinomatous lesions were significantly lower in group 6 (87%) than 7 (55%). Group 1, 2, 3, and 4 did not show any abnormalities of the bladder epithelium. These results confirm that NDGA have anti-tumor-promoting effects on bladder carcinogenesis in rats.