Pulmonary Hypoplasia and Morphological Characteristics and Surfactant Content Following Oligohydramnios or Diaphragmatic Hernia.

羊水过少或膈疝后的肺发育不全、形态特征和表面活性剂含量。

• 批准号: 01570913
• 负责人: HIGUCHI Motokazu
• 金额: $ 1.34万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570913/
• 关键词: pulmonary hypoplasia; oligohydramnios; diaphragmatic hernia; pulmonary surfactant; Fetus; oligohydramnios; Pulmonary hypoplasia; Pulmonary surfactant; pulmonary hypoplasia; Oligohydramnios; congenital diaphyragmatic hernia

To study the effect of oligohydramnios on lung growth and biochemical lung development in fetal rabbits, amniotic fluid was drained through a tube inserted into the maternal peritoneal cavity on the 23rd day of gestation. 1-ittermate fetuses without an amniotic shunt were used as controls. The fetuses were delivered abdominally on the 28th day of gestation. In a total of 8 pregnant does, 17 fetuses underwent amniotic shunting and 22 fetuses were used as controls. The amniotic shunt produced a significant reduction in the amniotic fluid volume. There were no differences in the wet weights of the fetal body, liver or brain between the two groups. however, the amniotic sliurit significantly decreased the wet weight of the fetal lung, fetal lung wet weight/body weight ratio, and protein concentration per lung as compared to tile control fetuses. In the fetal liver and brain tissues, no changes were found in the concentrations, of total phospholipids, phosphatidylcholine (PC) or disaturated phosphatidylcholine (DSPC, the main component of Iiing surfactant) per g of wet tissue and per mg of protein. however, the lungs of the fetuses with amniotic shunts contained significantly more PC and DSPC, and the IIS ratio was higher tlian in the control fetuses. These results suggest that the oligoliydramnios produced by an amniotic silunt causes pulmonary hypoplasia, but raises the pulmonary surfactant content of fetal rabbit lung.

为探讨羊水过少对胎兔肺生长和肺生化发育的影响，于妊娠第23天经母兔腹膜腔置管引流羊水。以未经羊水分流的1-胎龄胎儿为对照。这些胎儿是在怀孕第28天腹产的。在8只妊娠母猪中，17只接受羊水分流术，22只作为对照。羊水分流术使羊水量显著减少。两组胎儿的胎体、肝脏或脑的湿重没有差异。然而，与对照组相比，羊水组胎肺湿重、胎肺湿重/体重比和每肺蛋白质浓度显著降低。在胎肝和胎脑组织中，每克湿组织和每毫克蛋白质中总磷脂、磷脂酰胆碱(PC)或饱和磷脂酰胆碱(DSPC，其主要成分)的浓度没有变化。而羊水分流组胎儿肺组织中PC和DSPC含量明显高于对照组，而IIS比例高于对照组。这些结果表明，羊水过少可引起肺发育不良，但可增加胎兔肺表面活性物质的含量。

项目成果

MOTOKAZU HIGUCHI ET AL.: "The Influence of Experimentally Produced Oligohydramnjos on Lung Growth and Pulmonary Surfactant Content in Fetal Rabbit." J. of Developmental Physiology. 16. 223-227 (1992)

Motokazu Higuchi 等人：“实验生产的寡氢鼠尾草对胎兔肺生长和肺表面活性剂含量的影响”。

MOTOKAZU HIGUCHI ET AL.: "The Influence of Experimental Pulmonary Hypopiasia Due to Oligohydramnios on Lung Growth and Pulmonary Surfactant Production in Fetal Rabbit." J. of Japanese Medical Society for Biological Interface. 21. 184-185 (1990)

MOTOKAZU Higuchi 等人：“羊水过少引起的实验性肺发育不全对胎兔肺生长和肺表面活性剂产生的影响。”

M.Higuchi et al.: "The influence of pulmonary hypoplasia following experimentally produced oligohydramnios on lung growth and pulmonary surfactant production in fetal rabbits" J.developmental phisiology.

M.Higuchi 等人：“实验性羊水过少后肺发育不全对胎兔肺生长和肺表面活性物质产生的影响”J.发育生理学。

Motokazu Higuchi et al: "The influence of experimentally produced oligohydramnios on lung growth and pulmonary surfactant content in fetal rabbit" Journal of Developmental Physiology. 16. 223-227 (1992)

Motokazu Higuchi 等人：“实验性羊水过少对胎兔肺生长和肺表面活性物质含量的影响”《发育生理学杂志》。

Motokazu Higuchi et al: "The influence of experimental pulmonary hypoplasia due to oligohydramnios on lung growth and pulmonary surfactant production in fetal rabbit" Journal of Japanese Medical Society for Interface. 21. 184-185 (1990)

Motokazu Higuchi 等人：“羊水过少导致的实验性肺发育不全对胎兔肺生长和肺表面活性物质产生的影响”日本界面医学会杂志。