SOD and Catalase Activity in Oral Cancer Cell Line.

研究了抗癌药物对超氧化物歧化酶（SOD）的影响。本实验使用了两种癌细胞系，分别是HeLa S3细胞和NA细胞，它们起源于人类舌鳞癌，是1981年在我实验室建立的细胞系。研究了博来霉素、丝裂霉素C、顺铂和5-氟尿嘧啶作为抗癌药物。给药后，肿瘤细胞中SOD活性增强；在博莱霉素浓度为1.2 ~ 30马克杯/毫升时，SOD活性呈剂量依赖性增加，在给药2.0马克杯/毫升后6小时达到最大值。糖酵解抑制剂NaF在博莱霉素存在和不存在的情况下均能增强SOD活性，而KCN则显著降低SOD活性。NaF与博来霉素具有明显的叠加效应。这些结果表明，抗肿瘤药物可能通过多种机制增强SOD活性。

The effect of anticancer drugs on superoxide dismutase (SOD) was investigated. Two cancer cell lines were used in this experiment, they are HeLa S3 cells and NA cells which originated from a human squamous cell carcinoma of the tongue, were established as a cell line in our laboratory in 1981. Bleomycin, mitomycin C, cisplatin, and 5-fluorouracil were investigated as anticancer drugs. SOD activity in the cancer cells were found to be enhanced after administration of these drugs ; SOD activity increased in a dose-dependent menner at concentrations from 1.2 to 30 mug/ml of bleomycin, and reached maximam 6 hores after the administration of 2.0 mug/ml of the drug. NaF, a glycolysis inhibitor, enhanced SOD activity both in the presence and absence of bleomycin, while KCN decreased SOD activity significantly. NaF and bleomycin showed and additive effect. These results suggest that SOD activitymay be enhanced by anticancer drugs through several mechanism.