SOD and Catalase Activity in Oral Cancer Cell Line.

口腔癌细胞系中的 SOD 和过氧化氢酶活性。

• 批准号: 01571101
• 负责人: SAKURADA Shigeyo
• 金额: $ 1.22万
• 依托单位: Showa university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571101/
• 关键词: cancer cell line; anticancer drugs; superoxide dismutase; catalase; superoxide anion; bleomycin; superoxide dismutase; 口腔悪性腫瘍; 口腔癌由来株細胞

The effect of anticancer drugs on superoxide dismutase (SOD) was investigated. Two cancer cell lines were used in this experiment, they are HeLa S3 cells and NA cells which originated from a human squamous cell carcinoma of the tongue, were established as a cell line in our laboratory in 1981. Bleomycin, mitomycin C, cisplatin, and 5-fluorouracil were investigated as anticancer drugs. SOD activity in the cancer cells were found to be enhanced after administration of these drugs ; SOD activity increased in a dose-dependent menner at concentrations from 1.2 to 30 mug/ml of bleomycin, and reached maximam 6 hores after the administration of 2.0 mug/ml of the drug. NaF, a glycolysis inhibitor, enhanced SOD activity both in the presence and absence of bleomycin, while KCN decreased SOD activity significantly. NaF and bleomycin showed and additive effect. These results suggest that SOD activitymay be enhanced by anticancer drugs through several mechanism.

观察抗癌药物对超氧化物歧化酶(SOD)活性的影响。本实验采用HeLa S3细胞和人舌鳞状细胞癌NA细胞两种细胞系作为实验细胞系。研究了博莱霉素、丝裂霉素C、顺铂和5-氟尿嘧啶作为抗癌药物。肿瘤细胞中的超氧化物歧化酶活性在给药后被增强；博莱霉素在1.2-30µg/ml浓度范围内呈剂量依赖性增加，并在给药2.0µg/ml后达到最大值。糖酵解抑制剂NaF在有无博莱霉素的作用下均能显著提高SOD活性，而KCN则显著降低了SOD活性。氟化钠和博莱霉素表现出相加效应。这些结果提示，抗癌药物可能通过多种机制增强超氧化物歧化酶的活性。

项目成果

Yukari Gogun: "Enhancement of superoxide dismutase activity in cancer cell lines by anticancer drugs." Journal of Clinical Biochemistry and Nutrition. 8. 85-92 (1990)

Yukari Gogun：“抗癌药物增强癌细胞系中的超氧化物歧化酶活性。”

Yukari Gogun: "Enhancement of superoxide dismutase activity in cancer cell lines by anticancer drugs" J. Clin. Biochem. Nutr.8. 85-92 (1990)

Yukari Gogun：“抗癌药物增强癌细胞系中的超氧化物歧化酶活性”J. Clin。

五郡由佳里,桜田重世,木村義孝,南雲正男: "Enhancement of superoxide dismutase activity in cancer cell line by anticancer drugs" Journal of Clinical Biochemistry and Neutrition.

Yukari Gogori、Shigeyo Sakurada、Yoshitaka Kimura、Masao Nagumo：“抗癌药物增强癌细胞系中超氧化物歧化酶活性”临床生物化学与营养杂志。