Analysis of Oncogene Amplification and C-Myc Overexpression in Human Ovarian Cancer

人卵巢癌癌基因扩增和 C-Myc 过表达分析

• 批准号: 01571283
• 负责人: FUKUMOTO Manabu
• 金额: $ 0.77万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571283/
• 关键词: Human Tumor; Ovarian Cancer; Oncogene; Gene Amplification; Gene Expression; Tumor Tissue; C-myc

Amplification and expression of oncogenes were investigated in 55 cases of human ovarian cancer tissues. Gene amplificaion of unknown nature was screened by in-gel renaturation assay and amplification of the c-myc, Ki-ras and neu oncogenes were determined by Southern blot hybridization. No significant gene amplification was found in all the cases. By Northern hybridization assay, the c-myc gene was overexpressed in 29 cases (52.7%) whereas either k-ras or neu was not.The overexpression more than 3-fold of the c-myc gene was significant in serous cystadenocarcinoma and endometrioid carcinoma tissues compared with other histological types of tumor. Immunohistochemistry and in-situ hybridization revealed that tumor cells overexpressed both the c-myc gene and the protein.Although the expression level was not related to grading of malignancy, it is suggested that the c-myc gene is involved in early stages of carcinogesis of ovarian tumors.

对55例卵巢癌组织进行了癌基因扩增和表达的研究。用凝胶内复性法筛选未知性质的基因扩增，用Southern印迹杂交法检测c-myc、Ki-ras和neu癌基因的扩增。所有病例均未发现明显的基因扩增。北方杂交结果显示，c-myc基因过表达29例（52.7%），而k-ras和neu基因均不表达，其中浆液性囊腺癌和浆液性囊样癌的c-myc基因过表达率明显高于其他组织学类型。免疫组化和原位杂交结果显示，c-myc基因及其蛋白在卵巢肿瘤细胞中均呈高表达，其表达水平与卵巢肿瘤的恶性程度无关，但提示c-myc基因参与了卵巢肿瘤的早期癌变过程。

项目成果

Fukumoto,M.et al.: "Analysis of Gene Amplification in Human Tumor Cells by Inーgel DNA Renaturation Assay." Acta Heamatol.Jpn.52. 1463-1470 (1989)

Fukumoto, M. 等人：“通过 Ingel DNA 复性测定分析人类肿瘤细胞中的基因扩增。Acta Heamatol.Jpn.52 (1989)”。

Fukumoto,M.& Midorikawa,O.: "Molecular Biology of Cancer Genes" ed.by Sluyser,M.(Ellis Horwood Ltd.,London), (1990)

福本，M.

福本 学: "発癌の分子遺伝学" 日本産科婦人科熊本地方部会誌. 34. 93-99 (1990)

Manabu Fukumoto：“致癌的分子遗传学”日本妇产科熊本区杂志 34. 93-99 (1990)。

福本学: "癌化における遺伝子増幅" Medical Immunology. 18. 687-695 (1989)

Manabu Fukumoto：“癌化中的基因扩增”医学免疫学 18. 687-695 (1989)。

Fukumoto,M.et al.: "Association of Kiーras with Amplified DNA Sequences,Detection in Human Ovarian Carcinoma by a Modified Inーgel Renaturation Assay." Cancer Res.49. 1693-1697 (1989)

Fukumoto, M. 等人：“Kiras 与扩增 DNA 序列的关联，通过改良的 Ingel 复性测定检测人类卵巢癌。”49. 1693-1697 (1989)