Enzymatisch gesteuerte Benetzungsübergänge in zweidimensionalen dipolaren Langmuir-Filmen

二维偶极朗缪尔薄膜中酶促控制的润湿转变

• 批准号: 5235454
• 负责人: Professor Dr. Thomas Martin Fischer
• 金额: --
• 依托单位: Lehrstuhl für Experimentalphysik V
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2002-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5235454?language=en
• 关键词: Enzymatisch; gesteuerte; Benetzungs; berg; nge

The enzymatic control of two dimensional wetting in the three phase coexistence region liquid condensed/liquid expanded/ gaseous (LC/LE/G) of a Langmuir monolayer at the air/water interface shall be investigated using fluorescence microscopy combined with optical tweezers. Wetting transitions (complete towards partial wetting) shall be induced by adding enzymes to the subphase causing a change in surfactant composition in an initial two component (methyl-octadecanoate/phosphatidylinositol) monolayer mixture. Using the Langmuir monolayer as a model, the experiments shall reveal the possibility of a close interrelation between wetting nano-morphologies and protein activity in biological membranes, which originate from an electrostatically induced shape dependence of the line tensions between the coexisting phases.

利用荧光显微镜结合光学镊子研究了Langmuir单分子膜在空气/水界面处液相冷凝/液相膨胀/气相共存区（LC/LE/G）的二维润湿的酶促控制。在初始的两组分（十八酸甲酯/磷脂酰肌醇）单层混合物中，通过向亚相中添加酶引起表面活性剂组成的变化，可以诱导润湿转变（完全向部分润湿转变）。使用Langmuir单层作为模型，实验将揭示生物膜中润湿纳米形态和蛋白质活性之间密切相关的可能性，这源于静电诱导的共存相之间的线张力的形状依赖。