Development of Software for Automatic Analysis of Electric Signals of the Nerve Cells Recorded by Patch Clamp Method

膜片钳法记录神经细胞电信号自动分析软件的开发

• 批准号: 01870010
• 负责人: TAKAORI Shuji
• 金额: $ 4.35万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870010/
• 关键词: Electric signals of nerve cells; Software for analysis of electric signals; Patch clam method; Adrenal chromaffin cell; Muscarinic receptor; Potassium ion channel; Caudate nucleus; Dopamine D-2 receptor; 細胞電気信号; ソフトウェア; 副腎髄質; クロマフィン細胞; カリウムチャネ

We have studied the regulatory role of central monoaminergic and cholinergic nerves using electrophysiological method. Using mini-computor and our program developed recently, we are analyzing the data regarding current and voltage changes of the neurons recorded as well as the single channel activity. Furthermore, we developed software for supervision of the signals transferred from the electrophysiological equipments and automatic analysis with learning function. Using this system, the patch clamp studies were done to elucidate the mechanism underlying the muscarinic and nicotinic excitation of the adrenal chromaffin cells. In addition, the dopamine D-2 receptor-mediated inhibition from the substantia nigre of the caudate nucleus neuron receiving input from the cerebral cortex. In the first study, it was clarified that (1) excitation by acethylcholine of the adrenal chromaffin cells is mediated by both distinct muscarinic and nicotinic receptors, (2) the muscarinic excitation is induced by the inhibition of opening of potassium channels which are activated at near resting membrane potential, and (3) the inhibition of the potassium channel is due to the decrease of number of the channels activated. In the second study, it was demonstrated that dopamine derived from substantia nigre acts on D-2 receptors located on the nerve terminals from the cerebral cortex, thereby inhibiting the neuronal transmission from cerebral cortex to the caudate nucleus neurons.

我们用电生理方法研究了中枢单胺能神经和胆碱能神经的调节作用。利用微型计算机和我们最近开发的程序，我们正在分析有关记录的神经元的电流和电压变化以及单通道活动的数据。此外，我们还开发了用于监控电生理设备传输的信号并自动分析具有学习功能的软件。应用此系统进行了肾上腺嗜铬细胞的膜片钳研究，以阐明肾上腺嗜铬细胞的毒蕈碱和烟碱兴奋机制。此外，多巴胺D-2受体介导的抑制来自黑质的尾状核神经元接受来自大脑皮层的输入。在第一项研究中，阐明了（1）乙酰胆碱对肾上腺嗜铬细胞的兴奋是由不同的毒蕈碱和烟碱受体介导的，（2）毒蕈碱兴奋是通过抑制在接近静息膜电位时激活的钾通道开放诱导的，（3）钾通道的抑制是由于激活的通道数量减少。在第二项研究中，证明了来自黑质的多巴胺作用于位于来自大脑皮层的神经末梢上的D-2受体，从而抑制了从大脑皮层到尾状核神经元的神经元传递。

项目成果

Sasa M, Ohno Y, Takaori S: "Dopamine D-2 receptor-mediated inhibition from substantia nigre of caudate nucleus neurons activated by cortical stimulation." Brain Research.

Sasa M、Ohno Y、Takaori S：“多巴胺 D-2 受体介导的皮质刺激激活的尾状核神经元黑质抑制。”

Akaike A: "Voltage and current clamp studies of muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther. 255. 333-339 (1990)

Akaike A：“大鼠肾上腺嗜铬细胞毒蕈碱和烟碱激发的电压和电流钳研究。”

Akaike A: "A patch clamp study of muscarinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther. 255. 340-345 (1990)

Akaike A：“大鼠肾上腺嗜铬细胞毒蕈碱兴奋的膜片钳研究。”

Akaike A, Mine Y, Sasa M, Takaori S: "Voltage and current clamp studies of muscarinic and nicotinic excitation of the rat adrenal chromaffin cells." J Pharmacol Exp Ther. 255, (1). 333-339 (1990)

Akaike A、Mine Y、Sasa M、Takaori S：“大鼠肾上腺嗜铬细胞毒蕈碱和烟碱激发的电压和电流钳研究。”

Ujihara,H.: "Muscarinic regulation of spontaneously active vestibular neurons in vitro." Neurosci Lett. 106. 205-210 (1989)

Ujihara,H.：“体外自发活动前庭神经元的毒蕈碱调节。”