Studies on development of softwares to analyze electrical signals from neurons in the central nervous system.

我们在一台配有A/D转换器的微型计算机上开发了中枢神经系统电生理数据的支持分析软件。由于程序是在UNIX操作系统下用C语言编写的，所以软件可以根据实验目的方便地修改。该监测系统的程序可对数据进行快速、准确的分析，并能三维显示动作电位、兴奋性突触后电位（EPSP）和膜片钳单通道电流。用与计算机相连的A/D转换器将局部刺激诱发的EPSP数字化。将数字化数据显示在图形终端上，并绘制EPSP上升和衰减阶段数字化点的对数与时间的关系。利用上述计算机系统，进行了以下实验和数据分析， ...更多信息 1.用细胞内记录技术在脑片上观察了多巴胺对大鼠尾状核神经元的作用。用低浓度的多巴胺灌注浴<micrn>产生去极化，伴随着自发放电的增加和由施加到细胞中的去极化脉冲诱发的动作电位的数目的增加。与此相反，较高浓度（100 <micrn>M）的多巴胺抑制自发和电流诱导的放电，而对静息膜电位没有明显的影响。因此，可以认为抑制性D-1受体和兴奋性D-2受体同时存在于同一个尾状核神经元中。2.用大鼠尾状核切片研究了胆碱能激动剂和拮抗剂的作用，以阐明胆碱能系统在尾状核中的作用。结果表明，位于尾状核神经元突触前和突触后部位的M受体分别介导胆碱能抑制和兴奋。这些结果还提示，在尾状核内，乙酰胆碱引起的突触前抑制比突触后兴奋对突触传递的调节更占优势。少

We developed softwares for sutomatic analysis of the data obtained from electrophysiological studies in the central nervous system using a mini-computer equipped with an A/D converter. Since the program was written by "C" language under "UNIX" operating system, the sorfwares were easily altered according to the purpose of experiments. A rapid and accurate analysis of the data couls be made using programs for the monitoring system with three-dimensional display for action porentials, excitatory postsynaptic potential (EPSP) using an intracellular recording technique and single channel currents obtained by a patch clamp recording. EPSP induced by local stimulation was digitized using the A/D converter connected to the computer. The, digirized data were displayed on a graphic terminal, and the logatithms of the figitized points in the rise and decay phases of the EPSP were plotted against time. Using the computer system mentined above, following experiments and analysis of the data were c … More arried out.1. The effects of dopamine on the rat caudate nucleus neurons were examined in a slice preparation using an intracellular recording rechnique. Perfusion of the bath with a low concentration 1 <micrn>M) of dopamine produced a depolarization concomitant with an increase in the spontaneous firing and the number of action potentials evoked by a depolarizing pulse applied into the cells. In contrast, higher concentration (100 <micrn>M) of dopamine inhibited the spontaneous and current-induced firings without apparent effects on the resting membrane potential. Therefore, is was concluded that both inhibitory D-1 and excitatory D-2 receptors existed in the same caudate neuron. 2. The effects of cholinergic agonists and antagonists were investigated using slice preparations of the rat caudate nucleus to elucidate the role of the cholinergic system in the caudate nucleus. The results indicated that muscatinic receprots located on the presynaptic and postsynaptic sites of the caudate neutons mediated cholinergic inhibition and excitation, respectively. The data also implied that the presynaptic inhibition induced by acetuylcholine is much more dominant that the postsynaptic excitation with respect to the reguqlation of synapric transmissior in the caudate nucleus. Less