Studies on development of softwares to analyze electrical signals from neurons in the central nervous system.

研究开发分析中枢神经系统神经元电信号的软件。

• 批准号: 61870013
• 负责人: TAKAORI Shuji
• 金额: $ 4.67万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61870013/
• 关键词: Analysis of electrical signals; Softwares; Central neuron; EPSP; Caudate nucleus; Dopaminergic system; コリン作動系; 波形解析; uNIX; 細胞内記録; ドーパミン; ドーパミン受容体

We developed softwares for sutomatic analysis of the data obtained from electrophysiological studies in the central nervous system using a mini-computer equipped with an A/D converter. Since the program was written by "C" language under "UNIX" operating system, the sorfwares were easily altered according to the purpose of experiments. A rapid and accurate analysis of the data couls be made using programs for the monitoring system with three-dimensional display for action porentials, excitatory postsynaptic potential (EPSP) using an intracellular recording technique and single channel currents obtained by a patch clamp recording. EPSP induced by local stimulation was digitized using the A/D converter connected to the computer. The, digirized data were displayed on a graphic terminal, and the logatithms of the figitized points in the rise and decay phases of the EPSP were plotted against time. Using the computer system mentined above, following experiments and analysis of the data were c … More arried out.1. The effects of dopamine on the rat caudate nucleus neurons were examined in a slice preparation using an intracellular recording rechnique. Perfusion of the bath with a low concentration 1 <micrn>M) of dopamine produced a depolarization concomitant with an increase in the spontaneous firing and the number of action potentials evoked by a depolarizing pulse applied into the cells. In contrast, higher concentration (100 <micrn>M) of dopamine inhibited the spontaneous and current-induced firings without apparent effects on the resting membrane potential. Therefore, is was concluded that both inhibitory D-1 and excitatory D-2 receptors existed in the same caudate neuron. 2. The effects of cholinergic agonists and antagonists were investigated using slice preparations of the rat caudate nucleus to elucidate the role of the cholinergic system in the caudate nucleus. The results indicated that muscatinic receprots located on the presynaptic and postsynaptic sites of the caudate neutons mediated cholinergic inhibition and excitation, respectively. The data also implied that the presynaptic inhibition induced by acetuylcholine is much more dominant that the postsynaptic excitation with respect to the reguqlation of synapric transmissior in the caudate nucleus. Less

我们开发了一种软件，用于自动分析从中枢神经系统电生理研究中获得的数据，使用配备a /D转换器的小型计算机。由于程序是在UNIX操作系统下用C语言编写的，所以很容易根据实验目的对软件进行修改。利用三维显示动作电位、细胞内记录技术的兴奋性突触后电位（EPSP）和膜片钳记录获得的单通道电流的监测系统程序，可以对数据进行快速准确的分析。通过与计算机连接的A/D转换器对局部刺激诱发的EPSP进行数字化处理。将数字化的数据显示在图形终端上，并绘制出EPSP上升和衰减阶段数字化点随时间的对数。利用上述计算机系统，对数据进行了实验和分析。采用细胞内记录技术，观察了多巴胺对大鼠尾状核神经元的影响。低浓度（1 <micrn>M）的多巴胺浴液灌注产生去极化，同时细胞内的自发放电和动作电位数量增加。相比之下，较高浓度（100 <micrn>M）的多巴胺可抑制自发放电和电流诱发放电，对静息膜电位无明显影响。因此，我们认为在同一尾状神经元中存在抑制性D-1受体和兴奋性D-2受体。2. 利用大鼠尾状核切片研究胆碱能激动剂和拮抗剂的作用，以阐明胆碱能系统在尾状核中的作用。结果表明，位于尾状神经元突触前和突触后位置的麝香碱受体分别介导胆碱能抑制和兴奋。在尾状核突触传递的调控中，乙酰胆碱诱导的突触前抑制比突触后兴奋更占优势。少

项目成果

Ohno, Y.: "Coexistence of inhibitory dopamine D-1 and excitatory D-2 receptors in the same caudate nucleus neurons." Life Sciences. 40. 1937-1945 (1987)

Ohno, Y.：“抑制性多巴胺 D-1 和兴奋性 D-2 受体在同一尾状核神经元中共存。”

Sasa,M.: Brain Research. 420. 157-161 (1987)

Sasa，M.：大脑研究。

Sasa, M.: "Effects of L-threo-DOPS, an L-noradrenaline precursor, on locus coetuleus-originating neurons in spinal trigeminal nucleus." Brain Research. 420. 157-161 (1987)

Sasa, M.：“L-苏氨酸-DOPS（一种 L-去甲肾上腺素前体）对三叉神经脊髓核中的 coetuleus 源性神经元的影响。”

Akaike, A.: "Muscatinic inhibition as a dominant role in cholinergic regulation of transmission in the caudate nucleus." J. Ohatmacol Exp Ther. submitted.

Akaike, A.：“麝香抑制在尾状核传输的胆碱能调节中起主导作用。”

Akaike, A.: "Excitatory and inhibitory effects of dopamine on neutonal activity of the caudate nucleus nuerons in vitro." Brain Research. 418. 262-272 (1987)

Akaike, A.：“多巴胺对体外尾状核神经元中性活动的兴奋和抑制作用。”