权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Embryological studies on the genesis of ano-rectal malformations, using pig models.

使用猪模型对肛门直肠畸形的起源进行胚胎学研究。

基本信息

批准号：
02044024
负责人：
OHAKWA Haruo
金额：
$ 1.34万
依托单位：
Institute of Clinical Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02044024/
关键词：
Ano-rectal malformations Anal atresia Pig model with anal atresia Cloaca Cloacal plate Internal anal sphincter Three dimensional reconstruction 三次元立体構築三次元立体構築法 Cloacal plate dorsal cloaca cloac(総排泄腔)直腸尿道瘻

项目摘要

An ano-rectal malformation is one of the most important diseases in pediatric surgery. Various operative methods and their variations have been devised so far. But the operative results of high type of anomalies have not been necessarily satisfactory at all. We have tried to solve these problems from an embryological point of view.We have already established 2 families of pig model of ano-rectal malformations and have been capable to get anomalous pigs and foetuses from experimental matings. Pig foetuses of 25-60 days were obtained systematically, but there are still defective gestational ages of foetuses. They were supplemented by the materials of University of Utrecht by this co-opearative study, which had been produced from their already extinguished family.The results of the present studies are followed.1) Two pig families with malformations have been maintained by Tsukuba University team.2) Abnormal foetuses of 22-60 days were successfully obtained. But younger ages than 22 days w … More ere not obtained, and they were concluded to be impossible to get in the present method of abortion.3) Early embryological developments of anorectal malformations have been studied with these serially sectioned foetuses. They were studied in combination with those materials in the cooperative research with Utrecht University.4) Especially for the three dimensional reconstruction studies, over 1, 000 serial sections were necessary from each foetus.5) The progressive process of the partition between the ano-rectum and urogenital sinus from original cloacal system were ascertained. It was shown by the three dimensional pictures from our own devices. (Mebio 9(1), 1992)6) Classical explanation and concept of "cloacal membrane" was denied by our research. The defect of cloacal plate between the dorsal cloaca and tail groove were suggested to be a cause of anal atresia. This unsuccessful partition around 30 days in the case of pig is a cause of the anomaly.7) The inductive role of the cloacal plate was confirmed. But the true story must be confirmed by the materials before 21 days in the future.8) The development of internal sphincter was confirmed in normal abnormal pigs.9) The sex difference in the development of anal atresia were clearly explained by this research embryologically. Less

肛门直肠畸形是小儿外科最重要的疾病之一。到目前为止，已经设计了各种手术方法及其变体。但高型异常的手术效果却不一定令人满意。我们试图从胚胎学的角度来解决这些问题，已经建立了2个家系的猪肛门直肠畸形模型，并通过实验交配获得了畸形猪和畸形胎儿。系统地获得了25-60天的猪胎，但仍存在胎龄缺陷。本研究的结果如下：1）筑波大学的研究小组饲养了两个畸形猪家系; 2）成功地获得了22-60天的畸形胎儿。但年龄小于22天的妇女 ...更多信息 3）肛门直肠畸形的早期胚胎学发育已经用这些连续切片的胎儿进行了研究。特别是三维重建研究，每例胎儿需要1000多张连续切片; 5）明确肛门直肠与尿生殖窦从原始泄殖腔系统分隔的进展过程。这是由我们自己的设备的三维图片显示。（Mebio 9（1），1992）6）我们的研究否定了“泄殖腔膜”的经典解释和概念。泄殖腔背侧与尾沟之间的泄殖腔板缺损可能是肛门闭锁的原因之一。在猪的情况下，大约30天的这种不成功的分隔是异常的原因。7）泄殖腔板的诱导作用被证实。8）在正常异常猪中证实了内括约肌的发育; 9）本研究从胚胎学角度解释了肛门闭锁发生的性别差异。少