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Reserches on Anal Atresia related genes, genetic technology and human-pig genne mapping.

肛门闭锁相关基因、遗传技术及人猪基因图谱研究。

基本信息

批准号：
08457472
负责人：
OHKAWA Haruo
金额：
$ 4.22万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457472/
关键词：
Atresia ani Ano-rectal malformations Animal model Pig Disease related gene Microsatellite SSCP Comparative mapping 遺伝子 microsatellite

项目摘要

With 15 years'researches we have established our own pig family of ano-rectal malformations and have kept this with the help of the Grant-in-Aid for Scientific Reseach. Under the situation of continuous intra-family matings among anomalous pigs, the occurence rate of ano-rectal malformations increased gradually up to 75% in the matings between the anomalous pigs.From all the pig materials of the family, DNA material were extracted and preserved for the proceeding gene searches. Up to the present time this line of our study has not succeeded to clarify the requested genes so far, under SSCP and microsatellite analysis. The comparative gene maps between human and pig have developed in US Study Group and European Study Group separately. We have joined to European Group and have been developing new polymorphic markers ourselves. Some of the results were shown in "Mammalian Genome" : the relation between HSA-1 and SSC-6, SSC 14, and the relation between HSA-20 and SSC-17.In the past 2 years we were trying to complete the mating experiments through three generations between our pig family and the normal pigs from completely different primitive strain. We have not obtained enough numbers of anomalous pig material so far. But we are preceeding our gene targeting at the same time and hoping to reach the goal in the near future.

经过15年的研究，我们建立了自己的肛门直肠畸形猪家族，并在科学研究资助金的帮助下保持了这一点。在畸形猪家系内连续交配的情况下，畸形猪肛门直肠畸形的发生率逐渐升高，在家系间的交配中，畸形猪肛门直肠畸形的发生率高达75%。到目前为止，我们的研究还没有成功地澄清所要求的基因，到目前为止，通过SSCP和微卫星分析。人与猪的比较基因图谱分别由美国研究组和欧洲研究组完成。我们已经加入了欧洲集团，并一直在开发新的多态性标记自己。部分研究结果已在《哺乳动物基因组》上发表：HSA-1与SSC-6、SSC-14的亲缘关系，HSA-20与SSC-17的亲缘关系。到目前为止，我们还没有获得足够数量的异常猪材料。但我们同时也在推进基因打靶，希望在不久的将来实现这一目标。