Study on Autoregulatory Mechanism of Body Fluid with Special Emphasis on Kidney and Hormones

为了维持体液稳态，人体的自调节机制包括渗透压、电解质、酸碱平衡、营养物质、水分等，在人体中起着重要作用。为了阐明自身调控机制，特别是肾脏功能和激素的作用，本课题组成立。结果揭示了来自大鼠肾收集管的新型抗利尿激素敏感水通道的主要结构，噻嗪或阿米洛利与甲状旁腺激素对家兔连接管Ca2+吸收的相互作用，激素受体的定位及其沿大鼠肾细胞的信号转导，血管紧张素介导的大鼠肾小球系膜细胞信号转导，系膜功能和氯离子。血管加压素诱导的大鼠髓集管信号转导，一氧化氮诱导的大鼠低血压和尿钠/利尿，醛固酮受体的结构/功能关系，近端小管中药物转运质子协转运蛋白的调节，内皮素诱导的血管内皮和平滑肌细胞信号转导。

To maintain body fluid homeostasis, autoregulatory mechanism including osmolarity, electrolytes, acid-base balance, nutritents, water etc, plays important roles in human body. To elucidate autoregulatory mechanism, especially kidney function and hormone actions, the present research project team was organized. The results revealed the primary structure of a novel vasopressin-sensitive water channel from rat renal collecting duct, interaction of thiazide or amiloride with PTH in stimulating Ca2+ absorption in rabbit connecting tubulus, localization of hormone receptors and their signal transductions along rat nephron, angiotensin-mediated signal transduction in rat glomerular mesangial cells, mesangial function and chloride ions, vasopressin-induced signal transduction of rat medullary collecting duct, NO-induced hypotension and natriuresis/diuresis in rat, structure/function relationship of aldosteron receptor, regulation of proton cotranspoter for drug transport in proximal tubulus, and endothelins-induced signal transduction in vascular endothelial and smooth muscle cells.