Study on Autoregulatory Mechanism of Body Fluid with Special Emphasis on Kidney and Hormones

以肾与激素为重点的津液自动调节机制研究

• 批准号: 02304055
• 负责人: MARUMO Fumiaki
• 金额: $ 2.56万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304055/
• 关键词: autoregulation; renal function; hormones; body fluid; mesangium cell; tubular cell; signal transduction; water channel; エンドセリン; レセプター; バゾプレッシン; オ-トクリン; カルシウムイオン; 腎尿細管; cAMP; 体液調節; 腎; 内分泌; 高血圧; 利尿

To maintain body fluid homeostasis, autoregulatory mechanism including osmolarity, electrolytes, acid-base balance, nutritents, water etc, plays important roles in human body. To elucidate autoregulatory mechanism, especially kidney function and hormone actions, the present research project team was organized. The results revealed the primary structure of a novel vasopressin-sensitive water channel from rat renal collecting duct, interaction of thiazide or amiloride with PTH in stimulating Ca2+ absorption in rabbit connecting tubulus, localization of hormone receptors and their signal transductions along rat nephron, angiotensin-mediated signal transduction in rat glomerular mesangial cells, mesangial function and chloride ions, vasopressin-induced signal transduction of rat medullary collecting duct, NO-induced hypotension and natriuresis/diuresis in rat, structure/function relationship of aldosteron receptor, regulation of proton cotranspoter for drug transport in proximal tubulus, and endothelins-induced signal transduction in vascular endothelial and smooth muscle cells.

为了维持体液平衡，渗透压、电解质、酸碱平衡、营养物质、水分等自动调节机制在人体内起着重要作用。为了阐明自身调节机制，特别是肾功能和激素作用，本研究项目组成立。结果揭示了大鼠肾集合管中新型加压素敏感水通道的一级结构、噻嗪或阿米洛利与PTH在刺激兔连接小管Ca 2+吸收中的相互作用、激素受体的定位及其信号传导、激素受体及其信号传导沿着大鼠肾单位、血管紧张素介导的大鼠肾小球系膜细胞信号传导、系膜功能和氯离子、加压素诱导的大鼠延髓集合管信号转导、NO诱导的大鼠降压和利钠/利尿、醛固酮受体的结构/功能关系、近端小管质子共转运体对药物转运的调节以及内皮素诱导的血管内皮细胞和平滑肌细胞信号转导。

项目成果

Ujiie,K,Nonoguchi,H,Tomita,K,Marumo,F.: "Effects of ANF on cGMP synthesis" Am.J.Physiol. 259. F535-F538 (1990)

Ujiie,K,Nonoguchi,H,Tomita,K,Marumo,F.：“ANF 对 cGMP 合成的影响”Am.J.Physiol。

Ishikawa S,Okuda K,Saio T.: "Prompt inhibition of arginine vasopressinーinduced cellular cAMP production by extracellular sodium depletion in rat renal inner medullary collecting duct cells in culture" Endocrinology. 127. 560-566 (1990)

Ishikawa S、Okuda K、Saio T.：“培养的大鼠肾内髓集合管细胞中细胞外钠消耗对精氨酸加压素诱​​导的细胞 cAMP 产生的迅速抑制”内分泌学 127. 560-566 (1990)。

T.Okuno,H.Maegawa,K.Inui,R.Hori.: "Intraction of ofloxacin with organic cation transport system in rat renal brushーborder membranes" J.Pharmacol.Exp.Ther.,. 255. 1033-1037 (1990)

T.Okuno，H.Maekawa，K.Inui，R.Hori.：“氧氟沙星与有机阳离子转运系统在大鼠肾刷状缘膜中的引入”J.Pharmacol.Exp.Ther.，255。1033-1037（ 1990)

Marumo,F,Sakamoto,H,Ando,K,Ishigami,T.: "Concentrations of Atrial Natriuretic Peptide in Plasma and Urine of Kidney Disease Patients" Clinical Chemistry. 36. 1650-1653 (1990)

Marumo,F,Sakamoto,H,Ando,K,Ishigami,T.：“肾病患者血浆和尿液中心房钠尿肽的浓度”临床化学。

Fujiwara Y,Ochi S,Fukuhara Y,Yokoyama K,Fukunaga M,Shin S,Orita Y,Ueda N,Kamada T.: "Isotopic measurement of glomerular intracapillary volume as a quantitative index for mesangial cell contractility." Contribution to Nephrology.

Fujiwara Y、Ochi S、Fukuhara Y、Yokoyama K、Fukunaga M、Shin S、Orita Y、Ueda N、Kamada T.：“肾小球毛细血管内体积的同位素测量作为系膜细胞收缩性的定量指标。”