RENAL FUNCTION IN PROTEIN DEFICIENCY

蛋白质缺乏时的肾功能

• 批准号: 3229332
• 负责人: MANUEL MARTINEZ-MALDONADO
• 金额: $ 13万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3229332
• 关键词: adenosine monophosphate; aldosterone; angiotensins; atrial natriuretic peptide; diabetes insipidus; dietary constituent; glomerular filtration; guanosine monophosphate; hemodynamics; juxtaglomerular apparatus; kidney function; laboratory rat; norepinephrine; nutrition related tag; parathyroid hormones; prostaglandins; protein deficiency; renin

Protein malnutrition is a world wide problem. Malnutrition affects renal function, including reductions in renal plasma flow (RPF) and glomerular filtration rate (GFR). We have also found reduced peripheral renin (PRA) and aldosterone activity, resistance to the hypertensive effects of exogenous norepinephrine (NE) and increased sensitivity to the blood pressure-lowering effects of alpha blockade. The mechanisms involved in these changes remain largely unknown. The specific aim of this proposal is to establish the role played by various hormones and substances in this condition. Our data indicate that reduced the production of vasodilator prostaglandins and, possibly, increased sensitivity to the vasoconstrictor effects of angiotensin II and norepinephrine may be partly responsible for the reduced RPF, GFR and PRA. Also, low protein feeding of rats led to a fall in immunoreactive parathyroid hormone (iPTH), that may have curtailed the fall in GFR. We propose experiments to systematically examine the role of renin, angiotensin prostaglandins, NE, atrial natriuretic factor (ANF), antidiuretic hormone (ADH), and PTH on systemic and renal hemodynamics. Experiments will be conducted in rats fed low protein (6% casein) for two or sixteen weeks compared to controls pair-fed isocaloric normal protein (23% casein) diet. Balance, clearance and micropuncture studies (Munich-Wistar) will be done in intact, diabetes insipidus (Brattleboro) and parathyroidectomized rats. In addition, experiments will be conducted in isolated glomeruli and juxtaglomerular (granular) cells to determine the role of cyclic nucleotides (AMP and GMP), prostaglandins and various pharmacological agents in renin release. Radiocalcium fluxes and pharmacological manipulation of intracellular Ca mobilization will be performed in these preparations to determine the role of Ca. These experiments should provide important information regarding the systemic and renal hemodynamic changes associated with experimental and, possibly, human protein deficiency.

蛋白质营养不良是一个世界性的问题。营养不良影响

项目成果

Modulation of ANG II receptor and its mRNA in normal rat by low-protein feeding.

• DOI: 10.1152/ajprenal.1993.265.5.f660
• 发表时间: 1993
• 期刊: The American journal of physiology
• 作者: J. Benabe;S. Wang;J. Wilcox;M. Martínez‐Maldonado

Sequential changes in renal expression of renin-angiotensin system genes in acute unilateral ureteral obstruction.

急性单侧输尿管梗阻中肾素-血管紧张素系统基因的肾脏表达的连续变化。

• DOI: 10.1038/ki.1995.408
• 发表时间: 1995
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: PimentelJr,JL;Montero,A;Wang,S;Yosipiv,I;el-Dahr,S;Martínez-Maldonado,M
• 通讯作者: Martínez-Maldonado,M

Role of angiotensin II in the expression and regulation of transforming growth factor-beta in obstructive nephropathy.

血管紧张素II在阻塞性肾病转化生长因子-β的表达和调节中的作用。

• DOI: 10.1038/ki.1995.407
• 发表时间: 1995
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: PimentelJr,JL;Sundell,CL;Wang,S;Kopp,JB;Montero,A;Martínez-Maldonado,M
• 通讯作者: Martínez-Maldonado,M

Internal cRNA standards for quantitative northern analysis.

用于定量 Northern 分析的内部 cRNA 标准品。

• 发表时间: 1993
• 期刊: BioTechniques
• 影响因子: 2.7
• 作者: Wang,S;MurtaghJr,JJ;Luo,C;Martinez-Maldonado,M
• 通讯作者: Martinez-Maldonado,M

Renal renin, angiotensinogen, and ANG I-converting-enzyme gene expression: influence of dietary protein.

• DOI: 10.1152/ajprenal.1993.264.6.f981
• 发表时间: 1993-06
• 期刊: The American journal of physiology
• 作者: M. Martínez‐Maldonado;J. Benabe;Josiah N. Wilcox;Susheng Wang;Chuying Luo