Rhizobial Nod-Gene Induction By Released Signal form Host Plant

通过宿主植物释放的信号诱导根瘤菌Nod基因

• 批准号: 02804058
• 负责人: HIGASHI Shiro
• 金额: $ 1.02万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02804058/
• 关键词: Phizobium; nod-gene; Infection thread formation; Strain H1; H1 (nodA : : lacZ); Flavonoids; Gene expression; Root exudate; 根粒形成能; 感染能欠損株H1; H1(nodA::lacZ); 根毛カ-リング; 感染糸形成; 構築プラスミド; 発現ペプチド

The inducing effects of flavonoids on the lebel of nod gene transcription were examined. The expression induction was measured the beta-galactosidase activity in Rhizobium leguminosarum bv. trifolii H1 (nod218) which is introduced a fusion plasmid (pMudI 1734) including lacZ, into nodA poeron of R. bv. trifolii into Sym plasmid cured strain H1(Nod^-).Four flavonoids (7-hydroxyflavone, 7HF ; 4', 7-dihydroxyflavone, DHF : 4', 5, 7-trihydroxyflavone, apigenic ; 3', 4, 5, 7-tetrahydroxyflavone, luteolin) were tested for nod inducing activity. The beta-galactosidase activity of strain H1(nod218) was transiently expressed only in early logarithmic phase of its growth stages. All of them were effective on the lacZ expression at the same lebel. The maximum infection of bete-galactiosidase activity was caused by 5 mu M of flavonoid addition.The induction of nodA : : lacZ expression of strain H1(nod28) wes also detected with an exudate form intact seedidilings of Trifulium repens cv. Ladino (1-5 days afyer seeding).Moreover, the influences of flavonoids in the rhizosphere on in the rhizosphre on infection thread formation was also observed.

研究了黄酮类化合物对nod基因转录水平的诱导作用。以大豆根瘤菌Rhizobium leguminosarum bv. trifoliiiH 1（pM218），将含有lacZ的融合质粒（pMudI 1734）导入R. BV.以三叶草为材料，将4种黄酮类化合物（7-hydroxyflavone，7 HF; 4 '，7-dihydroxyflavone，DHF; 4'，5，7-trihydroxyflavone，apigenic ; 3 '，4，5，7-tetrahydroxyflavone，luteolin）转化到Sym质粒转化菌株H1（Nod^-）中，测定其诱导结瘤活性。菌株H1（H1218）的β-半乳糖苷酶活性仅在对数生长期早期瞬时表达。它们对lacZ基因表达的影响均在同一水平。5 μ M的类黄酮对β-半乳糖苷酶活性的诱导作用最大，用三叶草完整幼苗的分泌物也能检测到H1（H128）菌株的β-半乳糖苷酶A：：lacZ表达。此外，还观察了根际黄酮类化合物对根际侵染线形成的影响。

项目成果

Mikiko ABE: "Nodulation geneーexpression of Rhizobium leguminosarum bv.trifolii by flavonoids addion" Journal of General and Applied Microbiology.

Mikiko ABE：“添加类黄酮对豆科根瘤菌 bv.trifolii 的结瘤基因表达”《普通与应用微生物学杂志》。

Abe,M.,T.Nagai,S.Higashi,T.Uchiumi: "Role of flavonoids on early stage of nodulation of white clover with Rhizobium trifolii inoculation." Plant Physiology,.

Abe，M.，T.Nagai，S.Higashi，T.Uchiumi：“类黄酮对接种三叶根瘤菌的白三叶草结瘤早期的作用。”

Mikiko ABE,et al.: "Nodulation gene expression by flavonoids (in Jpn.)" Annual Reports on Plant-Microbe Interactions. 1. 27-29 (1991)

Mikiko ABE 等人：“黄酮类化合物的结瘤基因表达（日本）”植物-微生物相互作用年度报告。

Mikiko ABE, et al.: "Nodulation gene expression of Phizobium leguminosarum bv. trifolii by flavonoids addition." Journal of General and Applied Microbiology.

Mikiko ABE 等人：“通过添加类黄酮来表达豆类根瘤菌 bv. trifolii 的结瘤基因。”

Uchiumi,T.,S.Tachiwana,M.Abe,S.Higashi: "Transform to wide host range strain of Rhizobium fredii USDA193 by nodーgene of R. trifolii transfer." Journal of General and Applied Microbiology,.

Uchiumi, T., S. Tachiwana, M. Abe, S. Higashi：“通过 R. trifolii 转移转化为广泛宿主范围的根瘤菌 USDA193 菌株。”