Studies on the physiological mechanisms of isoflavonoids on the nod-gene expression of Bradyrhizobium japonicum

异黄酮对日本慢生根瘤菌nod基因表达的生理机制研究

• 批准号: 07660086
• 负责人: YAMAKAWA Takeo
• 金额: $ 1.73万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660086/
• 关键词: Rj-gene; Bradyrhizobium japonicum; nod-gene; isoflavonoids; glyceollins; genistein; daidzein; Nod metabolites; ジェニスタイン; 根粒形成遺伝子; ファイトアレキシン; グリセオリン; グリシノール

In order to investigate the point shared into ineffective or effective root nodules when soybean cultivars harboring Rj-gene were inoculated with a compatible or incompatible strain, we analyzed anatomically the nodule development in the roots of Rj_2Rj_3-and Rj_4-genotype soybean, CNS and Hill respectively, infected by Bradyrhizobium japonicum strains Is-1 (incompatible to CNS and compatible to Hill) and Is-34 (compatible to CNS and incompatible to Hill). The root hairs curlings were assayd by the staining method with methylene blue, the cortex divisions were examined with a light microscope, the invitation of rhizobia were investigated by the fluorescent antibody of rhizobia strains Is-1 or Is-34 with a fluorescence microscope. These results showed that the restriction mechanism of the infection with incompatible strains might operate at the step of rhizobia invasion into the primary meristem directly under cortex within 5 days after sowing and inoculation.On the assumption that the … More various composition of isoflavonoids were different in those soybean roots and the species of nod metabolites produced by genistein, daidzein and the root extracts were different among rhizobia strains, we searched a signal molecular which operate at the point shared intoineffective or effective root nodules. Daizein and glyceollin I contents in those soybean roots were level up to 7 days. Also the roots of CNS and Hill of 3 and 5 days' seedling inoculated with Is-1 or Is-34 were no difference in the contects of genistein and daidzen known as main isoflavonoids which induced the nod-gene expression of rhizobia. However a specific fluorescent spot was identified in the root extract of Hill and not in that of CNS.In order to analyze the composition and amount of nod metabolites, Is-1 and Is-34 were incubated using ^<14>C-acetate as tracer with genistein, daidzein, each soybean root extract or a specific fluorescent spot of Hill. These results shown that the separation pattern of nod metabolites of Is-1 or Is-34 were little difference among each treatment, but in comparison between Is-1 or Is-34, the number and intensity of nod metabolites were large difference. The chitinase treatment of those nod metabolites decreased the number of nod metabolites, thus it was confirmed that nod factors were included those nod metabolites. Hence, it was the different composition of nod metabolites between strains determined the compatibility of soybean varieties and B.japonicum strains, through different responses of host plant roots to nod metabolites. Less

为了探讨携带Rj基因的大豆品种接种亲和或不亲和菌株时，无效或有效根瘤的共有点，我们对Rj_2Rj_3-和Rj_4-基因型大豆、CNS和Hill的根瘤发育进行了解剖学分析。感染慢生根瘤菌菌株Is-1（与CNS不相容，与Hill相容）和Is-34（与CNS相容，与Hill不相容）。用亚甲蓝染色法观察根毛卷曲，光学显微镜观察皮层分裂，荧光显微镜观察根瘤菌Is-1和Is-34荧光抗体对根瘤菌的诱导作用。这些结果表明，不亲和菌株侵染的限制机制可能发生在根瘤菌在播种和接种后5天内侵入皮层下的初生分生组织这一步 ...更多信息 大豆根瘤菌根中根瘤菌的组成不同，染料木素、大豆苷元和根提取物产生的结瘤代谢产物种类也不同，我们寻找一个作用于无效或有效根瘤共有点的信号分子。大豆根中大豆苷元和大豆抗毒素I含量在7 d内保持稳定。接种Is-1和Is-34的3、5天幼苗的CNS和Hill根中，诱导根瘤菌表达外源基因的染料木素和大豆甙元的含量无明显差异。为了分析结瘤代谢物的组成和量，使用13 C-乙酸盐作为示踪剂，将Is-1和Is-34<14>与染料木素、大豆苷元、每种大豆根提取物或Hill的特异性荧光点一起孵育。结果表明，Is-1和Is-34的结瘤代谢产物的分离模式在各处理间差异不大，但在Is-1和Is-34之间比较，结瘤代谢产物的数量和强度差异较大。几丁质酶处理使结瘤代谢产物的数量减少，证实结瘤代谢产物中含有结瘤因子。因此，大豆品种与B.japonicum菌株间结瘤代谢产物组成的差异，通过寄主植物根系对结瘤代谢产物的不同反应，决定了大豆品种与B.japonicum菌株的亲和性。少

项目成果

Yoshimasa Nakano,Takeo Yamakawa,Motoki Ikeda,Junji Ishizuka: "Nodulation of Rj-Soybean Varieties with Rhizoblum fredii VSDA 193 under limited Supply of Nutrients." Soil Science and Plant Nutrition. 43(In press). (1997)

Yoshimasa Nakano、Takeo Yamakawa、Motoki Ikeda、Junji Ishizuka：“在营养物质供应有限的情况下，Rj-大豆品种与根瘤菌 VSDA 193 结瘤。”

Nakano, Y., Yamakawa, T., Ikeda, M.and Ishizuka, J.: "Nodulation of Rj-soybean varieties with Rhizobium fredii USDA 193 under limited supply nutritients." Soil Sci.Plant Nutr.43(In Press). (1997)

Nakano, Y.、Yamakawa, T.、Ikeda, M. 和 Ishizuka, J.：“Rj 大豆品种与根瘤菌 USDA 193 在营养供应有限的情况下结瘤。”

Yuichi Saeki,Takeo Yamakawa,Motoki Ikeda,Junji Ishizuka: "Effects of Roots exudatgs of Rj_2Rj_3-and Rj_4-genotype Soybeans on growth and Chemotaxis of Brady rhizobium japonicum." Soil Science and Plant Nutrition. 42・2. 413-417 (1996)

Yuichi Saeki、Takeo Yamakawa、Motoki Ikeda、Junji Ishizuka：“Rj_2Rj_3 和 Rj_4 基因型大豆的根分泌物对日本根瘤菌生长和趋化性的影响”42・2。 ）

Yuichi Saeki, Takeo Yamakawa, Motoki Ikeda, Junji Ishizuka: "Effects of root exudates of Rj_2 Rj_3-and Rj_4-genotype Soybeans on growth and Chemotaxis of Brady rhigobium Japonicum." Soil Science and Plant Nutrition. 42(In Press). (1996)

Yuichi Saeki、Takeo Yamakawa、Motoki Ikeda、Junji Ishizuka：“Rj_2、Rj_3 和 Rj_4 基因型大豆的根分泌物对日本慢生根菌生长和趋化性的影响。”

Saeki, Y., Yamakawa, T., Ikeda, M.and Ishizuka, J.: "Effects of roots exudates of Rj_2Rj_3-and Rj_4-genotype soybeans on growth and chemotaxis of Bradyrhizobiium japonicum." Soil Sci.Plant Nutr.42-2. 413-417 (1996)

Saeki, Y.、Yamakawa, T.、Ikeda, M. 和 Ishizuka, J.：“Rj_2Rj_3 和 Rj_4 基因型大豆根分泌物对日本慢生根瘤菌生长和趋化性的影响”。