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Continuous determination of Na concentration in CSF during recovery from thermal dehydration in rats.

连续测定大鼠热脱水恢复期间脑脊液中的钠浓度。

基本信息

批准号：
02807017
负责人：
HOSE Hiroshi
金额：
$ 1.02万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807017/
关键词：
Dehydration Rehydration Na electrode Cerebrospinal fluid Continuous measurement Naーelectrode

项目摘要

To determine changes in Na concentration in the cerebrospinal fluid([Na]csf)during restitution from thermal dehydration, we developed a method to measure[Na]csf continuously in rats using a double barreled Na sensitive electrode. The Na sensitive membrane was prepared by immobilizing an ionexchanger(Bis 12-crown-4). The confidence limit for measuring Na concentration was <plus-minus>0.5 meq/kg H20 within physiological range. We placed the electrode in the right lateral ventricle of an anesthetized rat thermally dehydrated by about 11% of body wt. Na concentration in plasma([Na]p)was measured by a flow cell typed Na sensitive electrode placed in an extracorporeal by-pass circuit between the left carotid artery and right jugular vein. We measured changes in[Na]p and[Na]csf continuously during the infusion of 2.5 ml/ lOOg body wt of distilled water for 10 min and following 20 min after the end of infusion. [Na]p began to decrease 3 min after the start of infusion and attained the lowest value of -11.6<plus-minus>0.9 meq/kg H_2O at 6 min after the end of infusion. This level was maintained until the end of experiment. [Na]csf decreased slowly after the start of infusion and at the end of experiment the change was -5.9<plus-minus>0.5 meq/kg H_2O. To assess the change in water and/or Na ion movement between blood and CSF in dehydrated rats, the response time of[Na]esf to change in[Na]p was compared between dehydrated(D)and enhydrated rats(E). The response time in E was 5.3<plus-minus>0.6 min which was significantly larger than in D, 2.0<plus-minus>0.5 min(p<0.01). These results suggest that[Na]csf showed attenuated and delayed response against acute change in[Na]p and recovered more slowly than[Na]p during rehydration. In addition. the movement of water and/or Na between blood and CSF was accelerated during rehydration.

为了测定热脱水恢复过程中大鼠脑脊液([Na]CSF)中钠离子浓度的变化，我们建立了一种用双管钠敏感电极连续测定大鼠脑脊液中钠离子浓度的方法。通过固定化离子交换剂(Bis 12-冠-4)制备了钠敏感膜。在生理范围内测量Na浓度的置信限为正负0.5meq/kg H20。我们将电极放置在热脱水约11%体重的麻醉大鼠的右侧脑室，在左侧颈动脉和右侧颈静脉之间的体外旁路中放置流动电池型钠敏感电极，测量血浆中钠浓度([Na]p)。分别于输注2.5ml/100g体重蒸馏水10min和输液结束后20min连续测定[Na]p和[Na]CSF的变化。[Na]p在输液开始后3min开始下降，6min时降至最低值，为-11.6±-0.9meq/kgH_2O。这一水平一直维持到实验结束。[NA]脑脊液在开始输液后缓慢下降，实验结束时变化为-5.9；+-0.5meq/kg H_2O。为了评价脱水大鼠血液和脑脊液中水和/或钠离子运动的变化，比较了脱水大鼠(D)和复水大鼠(E)的[Na]ESF对[Na]p变化的反应时间。E组的反应时间为5.3±0.6分钟，显著长于D组的2.0±0.5分钟(P&lt；0.01)。这些结果表明，在复水过程中，[Na]-CSF对[Na]p的急性变化表现出减弱和延迟的反应，并且恢复的速度慢于[Na]p。此外。在复水过程中，水和/或钠在血液和脑脊液之间的移动加快。