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Studies on analysis of ribosomal RNA gene organization, phylogeny and evolution of spirochetes

螺旋体核糖体RNA基因组织、系统发育和进化分析研究

基本信息

批准号：
02807046
负责人：
YANAGIHARA Yasutake
金额：
$ 0.96万
依托单位：
University of Shizuoka
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807046/
关键词：
Spirochetes Leptospira Borrelia Treponema Ribosomal RNA gene Cloning Sequencing リボソームRNA 遺伝子の構成塩基配列

项目摘要

In order to study the organization of the ribosomal RNA genes of some spirochetes the rRNA genes were cloned in phage lambda EMBL3. By subcloning the re striction fragments into pUC18 plasmid DNA and analyzing the resulting clones by Sourthern blot hybridization, a restriction map of the rRNA genes was generated and the organization of the rRNA genes was determined. The results obtained from pathogenic and nonpathogenic treponemes show that the genes for three types of rRNA (rrs, rrl, rrf) are closely linked and occur twice in their genome. Fukuna-ga et al., Microbiol. Immunol., 36, 161-167, 1992.Correlation of genomic hybridization data with physical charactedrization of recombinant plasmid DNAs of the rRNA genes in Borrelia burgdorferi, the etiological agent of Lyme disease, showed that, in contrast to the general organization of rDNA operons ineubacteria, the rrs gene is separated from the closely linked and tandemly repeated rrl/rrf genes. Direct repeat of rRNA genes in eubacterial … More genome is the first discovery and this is a remarkable feature in the organization of the eubacterial rRNA genes. Fukunaga et al., J. Gen. Microbiol., 138, in press and Fukunaga and Sohnaka, Biochem. Bioshys. Res. Commun., in press.To elucidate the organization of the transcription units encoding the rRNAs in the Leptospira interrogans, the nucleotide sequences of the genes and their flanking regions were determined, and the 5' termini of the transcripts were mapped by primer extention experiments. The results show that each of the rRNAs is transcribed separately, and the fact is consistent with the lack of physical linkage among them. This was the only organism known in the eubacteria, at that time, in which all the rRNA genes are present and expressed separately. We also found that there is no linkage among those in the organisms belonging to the genera Leptospira and Leptonema. Furthermore, the number of rrf gene in leptospires was correlated with their parasitism. In the strains of nonparasitic leptospires, there are two genes for rrf, whereas only one gene is carried in parasitic leptospires. Fukunaga et al., J. Bacteriol., 172, 32643268, 1990., Fukunaga et al., J. Gen. Microbiol., 137, 1523-1528, 1991. Less

为了研究某些螺旋体的核糖体RNA基因的组织，将rRNA基因克隆到λ噬菌体EMBL 3中。通过将限制性片段亚克隆到pUC 18质粒DNA中，并通过Sourmet印迹杂交分析所得克隆，产生rRNA基因的限制性图谱，并确定rRNA基因的组织。从致病性和非致病性密螺旋体获得的结果表明，三种类型的rRNA（rrs，rrl，rrf）的基因是紧密连锁的，并在其基因组中出现两次。Fukuna-ga等人，Microbiol.免疫学，36，161-167，1992.莱姆病的病原体伯氏疏螺旋体中rRNA基因的重组质粒DNA的物理特性与基因组杂交数据的相关性表明，与非细菌rDNA操纵子的一般结构相反，rrs基因与紧密连锁和串联重复的rrl/rrf基因分离。真细菌rRNA基因的直接重复序列 ...更多信息基因组是第一个发现，这是真细菌rRNA基因组织中的一个显著特征。Fukunaga等人，J. Gen. Microbiol.，138，出版中，以及Fukunaga和Sohnaka，Biochem. Bioshys.通信报告：为了阐明问号钩端螺旋体中编码rRNA的转录单位的结构，测定了该基因及其侧翼区的核苷酸序列，并通过引物延伸实验绘制了转录物的5'末端。结果表明，每个rRNA是单独转录的，这与它们之间缺乏物理联系是一致的。这是当时已知的真细菌中唯一一种所有rRNA基因都存在并单独表达的生物。我们还发现，在属于钩端螺旋体属和钩端螺旋体属的生物体中，它们之间没有连锁。钩端螺旋体rrf基因的数目与其寄生性有关。在非寄生性钩端螺旋体的菌株中，有两个rrf基因，而寄生性钩端螺旋体中只有一个基因。Fukunaga等人，J. Bacteriol.，172，32643268，1990.，Fukunaga等人，J. Gen. Microbiol.，137，1523-1528，1991。少