Molecular Genetics on Origin, Distribution and Control of Lyme Disease

莱姆病起源、分布和控制的分子遗传学

• 批准号: 06044191
• 负责人: YANAGIHARA Yasutake
• 金额: $ 5.89万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044191/
• 关键词: Lyme disease; Genetic analysis; OspA serotype; Transmission route; B.burgdorferi; B.gariniiii; B.afzelii; B.japonica; Ixodes ticks; Osp C; 病原性

1) Genomic DNAs of Lyme disease (LD) borreliae were digested with EcoRV of Hinc II and hybridized with probes specific for 23S rRNA genes and borreliae were classified into 10 types, I-X.2) From base sequences of OspC protein and flagellin genes, ribotype II,and IV-VI of Japanese isolates were classified as B.garinii.3) Main causative agents of Japanese LD were B.garinii and B.afzelii and main vector was I.Persulcatus. Main reservoir was Apodemus speciosus, and Clethrionomys rufocanus bedfordiae and migrating birds, Turdus chrysolaus and Emberiza spodocephala playd as reservoirs.4) Japanese LD borreliae were classified into 11 OspA serotypes based on reactivities to anti-OspA monoclonal antibodies. European and American isolates were classifed into 7 OspA serotypes. Only 1 type were identical each other.5) In Far East Russia, I.persulcatus harbored the organism at 26.6% (225/846). B.garinii and B.afzelii were isolated from ixodid ticks and rodents, genera Apodemus, Clethrionomys, Tamias, and Sorex, and their ratios were about 80% and 10%, respectively. No B.burgdorferi sensu stricto was found. Distribution pattern of Borrelia in Far East Russia was closely similar to that in Japan.6) B.japonica isolated from I.ovatus caused arthritis in SCID,C3H/HeN and ddY mice with collaborative works with Dr.Simon.7) Three borreliae isolated from I.tanuki, I.turdus and I.clumnae were suggested as new species, respectively.8) Borrelia, having 38kDa flagellin and 1 set of 23/5S rRNA genes, from I.persulcatus was distantly close to relapsing fever agent and named as B.miyamotoi.9) Meeting on the Cooperative Research was held in Ventura, U.S.A.on 22/23 Jan.1996 under the attendance of Drs.Johnson, Baranton, Simon, Wilske, Fukunaga, Masuzawa and Yanagihara and confirmed the success of this Joint Research.

1)用HincII的EcoRV消化莱姆病疏螺旋体的基因组DNA，与23S rRNA基因特异的探针杂交，将疏螺旋体分为10种类型。2)根据OspC蛋白和鞭毛基因的碱基序列，将日本分离株分为I-X型和IV-VI型。3)日本莱姆病疏螺旋体的主要病原是加里氏杆菌和阿氏疏螺杆菌，主要病原是Persulcatus。主要宿主为大姬鼠，红纹夜蛾和候鸟，金鱼和花斑斑潜蝇为主要宿主。4)根据对OspA单抗的反应性，将日本轮状疏螺旋体分为11个OspA血清型。欧美分离株可分为7个OspA血清型。5)在俄罗斯远东地区，全沟拟线虫的感染率为26.6%(225/846)。从硬蜱和啮齿动物、姬鼠属、节肢动物属、达米阿斯属和Sorex属中分离到加里尼氏杆菌和阿氏巴氏杆菌，分别约占80%和10%。未发现伯氏假单胞菌。从卵形螺旋体中分离到的疏螺旋体在俄罗斯远东地区的分布格局与日本极为相似。6)从卵形螺旋体分离的疏螺旋体引起SCID、C3H/母鸡和ddy小鼠的关节炎。7)分别从I.tanuki、I.turdus和I.clumnae分离的3个疏螺旋体被认为是新种。8)从I.perulcatus分离的疏螺旋体具有38 kDa鞭毛蛋白和1套23/5S rRNA基因，距离复发热原很近，命名为B.miyamotoi.9)合作研究会议在文图拉举行，1996年1月22日，在Johnson、Baranton、Simon、Wilske、Fukunaga、Masuzawa和Yanagihara博士的出席下，美国确认了这项联合研究的成功。

项目成果

川端寛樹、増澤俊幸、柳原保武: "臨床DNA診断法(古庄敏行、井村裕夫 監修、編集) スピロヘータ(ボレリア)" 金原出版, 1134 (1995)

Hiroki Kawabata、Toshiyuki Masuzawa、Yasutake Yanagihara：“临床 DNA 诊断方法（由 Toshiyuki Furusho 和 Hiroo Imura 监督和编辑）Spirochete (Borrelia)” Kanehara Publishing，1134 (1995)

Toshiyuki Masuzawa: "Infectivity and early antibody-response to Borrelia burgdorferi sensu lato isolated in Japan in outbred mice" Microbiology and Immunology. 38. 621-627 (1994)

Toshiyuki Masuzawa：“日本在远交小鼠中分离出的伯氏疏螺旋体的感染性和早期抗体反应”微生物学和免疫学。

Toshiyuki Masuzawa: "Identification of spirochetes isolated from wild rodents in Japan as Borrelia japonica" Journal of Clinical Microbiology. 33(5). 印刷中 (1995)

Toshiyuki Masuzawa：“从日本野生啮齿动物中分离出的螺旋体为日本疏螺旋体”，《临床微生物学杂志》33(5)，出版中。

Masahito Fukunaga: "Characterization of unusual spirochetes of the genus Borrelia isolated from ixodid ticks,I*odes persulcatus,in Japan" Journal of Bacteriology. (submitted).

Masahito Fukunaga：“日本从硬蜱（I*odes persulcatus）中分离出的疏螺旋体属异常螺旋体的特征”《细菌学杂志》。

宮本健司: "ダニと疾患のインターフェイス" Yuki書房.SADI組織委員会編, 179 (1994)

Kenji Miyamoto：“蜱虫与疾病之间的界面”Yuki Shobo，SADI 组织委员会编辑，179（1994）。