Studies on Heterogeniety of General Transcription Factor TFIID

总转录因子TFIID异质性研究

• 批准号: 02808051
• 负责人: TAMURA Taka-aki
• 金额: $ 1.02万
• 依托单位: Saitama Medical School (1991)Okazaki National Research Institutes (1990)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02808051/
• 关键词: Transcription factor; Transcriptional regulation

The core promoter of the mouse myelin basic protein (MBP) gene from -36 to +12 was tissue-specifically (brain) transcribed. Biochemical studies demonstrated that TFIID fraction contains factors involved in such a specific transcription. Tnus. we hypothesized the existence of a tissuespecific TFIID. We isolated a cDNA clone for TFIID from the mouse brain, and compared its structure with the human TFIID. Few minor differences were observed only in the N-terminal portion of the mouse TFIID. The C-terminus that is required for the TFIID function was identical among two TFIIDs. Only a single TFIID gene was detected in mouse genome. Northern blot analysis demonstated that the TFIID transcript is expressedas the same size RNAs in mouse tissues. Mutation analysis within the MBP core promoter showed that the TATA-box is not essential but TATA-flanking sequences were important for tissue-specific transcription. From these results, there may be no tissue-specific TFIID. In these days, TFIID associating factors (TAF) have been identified by other groups. In our case, it is suggested that a tissue-specific TAF can be involved in the core promoter-mediated tissue-specific transcription.

小鼠髓鞘碱性蛋白（MBP）基因的核心启动子从-36到+12是组织特异性（脑）转录的。生物化学研究表明，TFIID组分含有参与这种特异性转录的因子。Tnus。我们假设存在组织特异性TFIID。我们从小鼠脑中分离了TFIID的cDNA克隆，并将其结构与人TFIID进行了比较。仅在小鼠TFIID的N-末端部分观察到很少的微小差异。TFIID功能所需的C-末端在两个TFIID中是相同的。在小鼠基因组中只检测到一个TFIID基因。北方印迹分析表明TFIID转录本在小鼠组织中以相同大小的RNA表达。MBP核心启动子内的突变分析表明，TATA盒是不是必不可少的，但TATA侧翼序列是重要的组织特异性转录。根据这些结果，可能没有组织特异性TFIID。在这些日子里，TFIID相关因素（TAF）已被其他小组确定。在我们的情况下，这表明，组织特异性TAF可以参与核心启动子介导的组织特异性转录。

项目成果

田村 隆明,他: "Sequences involved in brainーspecific in vitro transcription from the core promoter of the mause myelin basic protein gene" Biochimica et Biophysica Acta. 1129. 83-86 (1991)

Takaaki Tamura 等人：“来自小鼠髓磷脂碱性蛋白基因核心启动子的脑特异性体外转录涉及的序列”Biochimica et Biophysicala Acta. 1129. 83-86 (1991)

Taka-aki Tamura et al.: "Sequences in evolved in brain-specifc in vitro transcription from the core promoter of the mouse myelin basic protein gene." Biochim. et Biophys. Acta. 1129. 83-86 (1991)

Taka-aki Tamura 等人：“从小鼠髓磷脂碱性蛋白基因的核心启动子进化而来的大脑特异性体外转录序列。”

田村 隆明,他: "Striking homology of the “Variable" Nーterminal as well as the “conserved" domains of the mouse and human TATAーfactors(TFIID)" Nucleic Acids Research. 19. 3816-3865 (1991)

Takaaki Tamura 等人：“小鼠和人类 TATA 因子 (TFIID) 的“可变”末端以及“保守”结构域的惊人同源性”《核酸研究》19. 3816-3865 (1991)。

田村 隆明,他: "Striking homology of the ‘Variable'Nーterminal as well as the ‘Conserved' domains of the mouse and human TATAーfactors(TFIID)" Nucleic Acids Research. 19. 3816-3865 (1991)

Takaaki Tamura 等人：“小鼠和人类 TATA 因子 (TFIID) 的‘可变’N 末端以及‘保守’结构域的惊人同源性”《核酸研究》19. 3816-3865 (1991)。

Taka-aki Tamura et al.: "Striking homology of the 'variable' N-terminal as well as the 'conserved' domains of the mouse and human TATA-factors (TFIID)." Nucleic Acids Research. 19. 3816-3865 (1991)

Taka-aki Tamura 等人：“小鼠和人类 TATA 因子 (TFIID) 的‘可变’ N 端以及‘保守’结构域具有惊人的同源性。”