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Neuronal death and sex hormones during brain sexual differentiation

大脑性别分化过程中的神经元死亡和性激素

基本信息

批准号：
02640585
负责人：
ARAI Yasumasa
金额：
$ 1.22万
依托单位：
Juntendo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640585/
关键词：
Brain sexual differentiation sex steroid cell death sex difference LHRH neurons cell migration olfactory placodal origin preoptic area 性ホルモン嗅枝 NCAM 神経細胞死性的二型性 ANP チロシン水酸化酵素エストロゲン受容体

项目摘要

During the process of brain sexual differentiation, sex steroids regulate the number of certain neuronal groups. Recently, we have found that the anteroventral periventricular nucleus of the preoptic area (AVPVN-POA) is sexually dimorphic and perinatal treatment of female rats with androgen decreases the neuronal number of the AVPVN-POA, resulting in malefemale difference in nuclear volume.In the present study, we injected Bromodeoxyuridine (BrdU) into pregnant rats at day 15 of pregnancy in order to label the proliferating neurons with BrdU. Pregnant mothers were also injected with androgen from days 14 to 18. Animals were sacrificed at day 17 or 21Exposure of androgen to female fetuses from. !days 14 to 18 markedly reduced BrdU-labeled neurons in the AVPVN area, compared to control female fetuses, when sacrificeed, . at day : 21 of pregnancy, whereas there- was no differenced when. sacrificed at day 17. BrdU labelled neurons in the AVPVN-POA was also significantly smaller than that in control females at day 21.Incidence of pycnotic figures in the AVPVN-POA was significaltly higher in males and androgenized females than control females, when sacrificed at day 21.These results suggest that gndrogengiven prenatally regulates the neuron number of the AVPVN=POA through enhancing neuronal death.other than the regulation of neuronal number by cell death, neuronal migration phenomenon is one of the influencing factors for neuronal number of. the nuclear groups. In the present study, we have studied the origin and migration phenomenon of LHRH neurons from the olfactory placode to the forebrain area in the chick embryos. In this study, the possible participation of a highly polysialylated NCAM in migration of LHRH neurons from the nasal region to the forebrain area.

在脑性别分化过程中，性类固醇激素调节某些神经元群的数量。最近，我们发现，前腹侧视前区室周核（AVPVN-POA）是性二态性的，围产期给予雄激素治疗的雌性大鼠减少了AVPVN-POA的神经元数量，导致核体积的雄性差异。怀孕的母亲也注射雄激素从第14天至18天。在雄激素暴露于雌性胎仔的第17天或第21天处死动物。!与对照组雌性胎儿相比，第14 - 18天的AVPVN区域中BrdU标记的神经元显著减少。在妊娠第21天，而在何时没有差异。在第17天处死。AVPVN-POA内BrdU标记的神经元在21天时也显著小于对照组雌性。雄性和雄激素化雌性AVPVN-POA内固缩影的发生率显著高于对照组雌性，这些结果表明，孕激素通过促进神经元死亡来调节AVPVN=POA的神经元数量。除了通过细胞死亡来调节神经元数量外，神经元迁移现象是影响神经元数量的因素之一。核集团。本文研究了鸡胚嗅板LHRH神经元向前脑区的起源和迁移现象。在这项研究中，高度多聚唾液酸化的NCAM可能参与LHRH神经元从鼻区迁移到前脑区。