Migration of LHRH neurons born in the olfactory placode into the forebrain in the chick embryo

鸡胚嗅基板中产生的 LHRH 神经元迁移至前脑

基本信息

  • 批准号:
    05680704
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

(1)The epithelial cells of the olfactory placode of the chick embryoswere labeled with a fluorescent carbocyanine dye DiI at embryonic days 3.5-4.0. The DiI-labeled cells were first detected in the olfactory epithelium and olfactory nerve one day after the application of DiI.Two or four days after the dye application, the labeled cells sequentially appeared in the rostral medial forebrain, and in the septo-preoptic area. The distribution pattern of DiI-labeled cells closely resembled that of LHRH neurons. Double staining for DiI and LHRH demonstrated that DiI-labeled cells co-expressed LHRH.These results provide evidence for the actual migration of LHRH neurons from the olfactory placode to the septo-preoptic area during the development.(2)In the embryos with a incomplete placodectomy, a small number of LHRH-immunoreactive (ir) cells expressing NCAM-H were detected in the olfactory epithelial fragments and in the NCAM-H-positive olfactory nerve remnants which ceased to extend their axons to the forebrain. The lack of the central projection of the olfactory nerve caused stagnation of LHRH-ir cells, no LHRH-ir cells being found in the forebrain. Furthermore, the migrating LHRH-ir cells deviated from the poorly developed olfactory nerve and migrated into the NCAM-H-positive medial nasal branch of the ophthalmic nerve of the trigeminal nerve. These results suggest the importance of the structual support for the migration of LHRH neurons, and that the migration route and target of LHRH neurons are not exactly programd in the precursor cells of LHRH neurons in the olfactory placode. Since the migrating LHRL-ir cells were never independent of NCAM-H-positive neural elements in vivo and in vitro, the possible interaction between the migration of LHRH neurons and NCAM-H may be needed for successful LHRH neuronal migration.
(1)在胚胎期3.5 ~ 4.0天,用荧光碳青素染料DiI标记鸡胚嗅觉基板上皮细胞。DiI标记的细胞在给药1天后首次在嗅上皮和嗅神经中检测到。染色后2天或4天,标记细胞依次出现在前脑吻侧内侧和中隔-视前区。dii标记细胞的分布模式与LHRH神经元非常相似。DiI和LHRH的双染色表明,DiI标记的细胞共表达LHRH。这些结果为LHRH神经元在发育过程中从嗅觉区实际迁移到隔视前区提供了证据。(2)在不完全切除胎盘的胚胎中,在嗅上皮碎片和停止向前脑延伸轴突的NCAM-H阳性嗅神经残体中检测到少量表达NCAM-H的lhrh免疫反应(ir)细胞。嗅觉神经中央投射的缺失导致LHRH-ir细胞的停滞,在前脑中没有发现LHRH-ir细胞。此外,迁移的LHRH-ir细胞偏离发育不良的嗅神经,迁移到ncam - h阳性的三叉神经眼神经内鼻支。这些结果提示了LHRH神经元迁移的结构支持的重要性,并且LHRH神经元的迁移途径和目标并不完全在嗅觉基因位的LHRH神经元前体细胞中被编程。由于迁移的LHRL-ir细胞在体内和体外从未独立于NCAM-H阳性神经元件,因此LHRH神经元迁移与NCAM-H之间可能存在相互作用,这可能是LHRH神经元迁移成功的必要条件。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Miyakawa,M.: "Developmental changes of NCAM-H and cytoskelton in LHRH-expressing migrating cells from the olfactory placode to the brain." Neuroscience Research. S18. S110 (1993)
Miyakawa,M.:“表达 LHRH 的细胞从嗅觉基板迁移到大脑时 NCAM-H 和细胞骨架的发育变化。”
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    0
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新井康允: "GnRHニューロンの組織発生-嗅上皮から脳内への移動" Hormone Frontier in Gynecology. 1. 13-21 (1994)
Yasunori Arai:“GnRH 神经元的组织发育 - 从嗅觉上皮到大脑的迁移”《妇科激素前沿》1. 13-21 (1994)。
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    0
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新井康允: "高シアル酸化したNCAM(NCAM=H)の神経系の発生及び成体でのニューロン新生における役割" 生体の科学. 44. 285-291 (1993)
Yasutoshi Arai:“高度唾液酸化的 NCAM (NCAM=H) 在神经系统发育和成人神经发生中的作用”《生物科学》44. 285-291 (1993)。
  • DOI:
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  • 影响因子:
    0
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Murakami,S.: "Direct evidence for the migration of LHRH neurons from the nasal region to the forebrain in the chick embryo:a carbocyanine dye analysis." Neurosci Res. 19. 331-338 (1994)
Murakami,S.:“鸡胚中 LHRH 神经元从鼻区迁移到前脑的直接证据:碳花青染料分析。”
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Murakami.S.: "Direct evidence for the migration of LHRH neurons from the nasal region to the forebrain in the chick embryo:a carbocyanine dye……" Neuroscience Research. 19(in press). (1994)
Murakami.S.:“鸡胚胎中 LHRH 神经元从鼻区迁移到前脑的直接证据:碳花青染料......”《神经科学研究》19(出版中)。
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ARAI Yasumasa其他文献

ARAI Yasumasa的其他文献

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{{ truncateString('ARAI Yasumasa', 18)}}的其他基金

Migration of LHRH neurons from nasal to forebrain region--an analysis of molecular mechanisms of neuronal migration
LHRH神经元从鼻腔向前脑区的迁移--神经元迁移的分子机制分析
  • 批准号:
    07680880
  • 财政年份:
    1995
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Neuronal death and sex hormones during brain sexual differentiation
大脑性别分化过程中的神经元死亡和性激素
  • 批准号:
    02640585
  • 财政年份:
    1990
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
A developmental biological analysis: Neurotropic effects of estrogen on the perinatal hypothalamic tissue transplanted into the adult rat brain.
发育生物学分析:雌激素对移植到成年大鼠大脑的围产期下丘脑组织的神经营养作用。
  • 批准号:
    61570031
  • 财政年份:
    1986
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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