Molecular cloning of photolyase gene in soybean.

以大豆（Glycine max L.）此外，还构建了含有预扩增的大肠杆菌和酿酒酵母光解酶基因同源物的质粒“富集cDNA”文库。通过在大肠杆菌和S.从酿酒酵母中分离出两种编码高等植物光裂合酶的克隆。这两个克隆均不能转化光复活缺陷的葡萄球菌细胞。从克隆A的核苷酸序列推导的氨基酸序列与脊椎动物的氨基酸序列显著不同，但与大肠杆菌和链球菌的氨基酸序列相似。所有已知光解酶中保守的氨基酸残基在大豆光解酶中完全保守。

lambdaZAP cDNA library was constructed from mRNA of grean leaves of soybean Glycine max L. In addition, plasmid "enriched cDNA" library with pre-amplification of homologes of Escherichia coli and Saccharomyces cerevisiae photolyase genes were also constructed for the same source. By introducing and expressing them in E.coli and S. cerevisiae, two types of clones encoding photolyase of the higher plant were isolated. Both of clones did not transform photoreactivation defficient cells of S.cerevisiae. The amino acid sequence deduced from the nucleotide sequence of clone A differs significantly from that of the vertibrate, but is similar to those of E.coli and S.cerevisiae. Amino acid residues conserved in all the known photolyases are perfectly conserved in soybean photolyase.