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A study on detoxication of dietary products of lipid peroxidation in digestive tract.

膳食制品对消化道脂质过氧化的解毒作用研究

基本信息

批准号：
02660095
负责人：
KANAZAWA Kazuki
金额：
$ 1.6万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660095/
关键词：
Lipid Peroxidation Products Detoxify in Digestive Tract Detection of Peroxidation Products Nutrition Linoleic Acid Hydroperoxide Trilinolein Hydroperoxide Toxicity of Lipoperoxide Incorporation into Body ペルオキシドの分解過酸化物のHPLC 胃内解毒食餌脂質

项目摘要

Detoxifying ability of digestive tract for dietary products of lipid peroxidation was investigated in rats. The method for quantitative detection of peroxidation products from the animal stomach and intestines was estimated using a HPLC equipped with an amperometer and a UV detector. Linoleic acid hydroperoxides dosed orally easily disappeared in the stomach. A part of them was reduced to hydroxy linoleic acid or decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The others were changed to keto-epoxy-octadecaenoic acid and remained in the gastric membrane. When trilinolein hydroperoxides were dosed orally, they disappeared in the stomach more slowly than linoleic acid hydroperoxides. Because trilinolein hydroperoxides were hydrolyzed to linoleic acid hydroperoxides in the stomach by lipase, it was considered that they were also decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The disappearing rate of trilinolein hydroperoxides in the stomach was not affected by the nutritional conditions of rats on such a diet as high-lipid, high-protein, or high-carbohydrate. Thus, lipid hydroperoxides were decomposed in the stomach to aldehydes, which were incorporated into the body. It was therefore concluded that the toxicity of dietary products of lipid peroxidation must be due to their aldehydic products. I have already been made clear the hepatotoxicity by dietary aldehydes in the rats. By this project, a major components of the aldehydic products, 9-oxononanoic acid, was determined to be incorporated into liver. Then, another study on a role of 9-oxononanoic acid as one of bio-signals in the body is in progress.

以大鼠为实验对象，研究其对膳食脂质过氧化产物的解毒作用。使用配有安培计和紫外检测器的高效液相色谱对动物胃和肠道中过氧化产物的定量检测方法进行了评估。口服亚油酸过氧化氢很容易在胃里消失。其中一部分被还原为羟基亚油酸或分解为醛，然后转移到空肠并结合到体内。其余部分为酮基-环氧十八烯酸，并留在胃膜内。当口服过氧化氢三甘油酯时，它们在胃中的消失速度比亚油酸过氧化氢要慢。由于三亚油酸过氧化氢在胃中被脂肪酶降解为亚油酸过氧化氢，因此认为它们也被分解成醛，然后转移到空肠并结合到体内。高脂、高蛋白、高碳水化合物饮食大鼠的营养状况不影响胃内过氧化氢三甘油酯的消失率。因此，脂质过氧化氢在胃中被分解成醛，并被结合到体内。由此可见，膳食脂质过氧化产物的毒性可能源于其醛类产物。我已经清楚了饮食中的醛对大鼠的肝脏毒性。通过该项目，确定了醛类产品中的主要成分9-氧辛酸被引入肝脏。然后，另一项关于9-氧杂环酸作为生物信号在体内的作用的研究正在进行中。