Synthesis of Fungal potent auxins, accremoauxins, and their applied study on initiation and propagation of frail calli of conifers.

真菌强效生长素、accremoauxins的合成及其在针叶树脆弱愈伤组织引发和繁殖中的应用研究。

• 批准号: 02660128
• 负责人: SASSA Takeshi
• 金额: $ 1.41万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660128/
• 关键词: acremoauxins; syntheses of acremoauxins; 2-(3-indolyl) propionic acid; propagation of Sugi calli; plant growth-inhibiting activity; synthetic auxins; スギカルス; インド-ルプロピオン酸; 新オ-キシン物質; カルスの増殖

1)Acremoauxin A(AA-A), a new potent auxin derivative produced by Acremonium roseum I4267, was synthesized from 2, 3 : 4, 5-di-0-isopropylidene-D-arabitol prepared from Dmannitol and(+)-2-(3-indolyl)propionic acid[(+)-IPA, obtained from(<plus-minus>)-IPA by biological resolution using A. roseum I42671. The synthetically confirmed structure of AA-A was 1-0-[(2S)-2-(3-indolyl)propionyll-D-arabitol. Its related compound, AA-B chromatographically detected from this fungus, was similarly synthesized from the diisopropylidene-D-arabitol and(3-indolyl)acetic acid(IAA), and synthetic AA-B was in fair agreement with natural one in TLC, HPLC and ^1H-NMR.2)Effects of the var4-ous auxins including AA-A with or without BA on the growth of the calli from Sugi and Akamatsu were investigated on the Murasige & Skoog's media. Results indicated the following orders in activities of callus initiation and propagation. Explant : Sugi triploid>its diploid>Akamatsu, auxins : NAA, 2, 4-D>IBA, 5, 6-DIAA>IPA, AA-A, IAA.3)Effects of AA-A and the enantiomers of IPA on the growth of plant seedlings were examined. AA-A markedly inhibited the growth of the seedlings of Chinese cabbage, lettuce and oat, but its inhibitory activity against these seedlings was weaker than that of IPA. In tests with 5 species of plant seedlings, (+)-IPA showed the growth inhibition higher than that of its enantiomer. Two seedlings of rice and radish, however, were highly inhibited in the growth by(-)-IPA rather than(+)-IPA.

1)由D-甘露醇制得的2，3：4，5-二-O-异亚丙基-D-阿拉伯糖醇与（+）-2-（3-吲哚基）丙酸[（+）-IPA]经生物拆分得到Acremoauxin A（AA-A）<plus-minus>。玫瑰I42671。AA-A的合成确认结构为1-0-[（2S）-2-（3-吲哚基）丙酰基]-D-阿拉伯糖醇。其相关化合物AA-B通过色谱从该真菌中检测到，类似地从二异亚丙基-D-阿拉伯糖醇合成，（3-吲哚基）乙酸（IAA）和合成AA-B在薄层色谱中与天然产物一致，2）以日本杉和赤松的愈伤组织为材料，研究了不同浓度的AA-A和BA对愈伤组织生长的影响。Skoog的媒体。结果表明，愈伤组织的诱导和增殖活性顺序为：取出：生长素：NAA、2，4-D&gt;IBA、5，6-DIAA&gt;IPA、AA-A、IAA。AA-A对大白菜、莴苣和燕麦幼苗生长有明显的抑制作用，但抑制作用弱于IPA。对5种植物幼苗的生长抑制试验表明，（+）-IPA的生长抑制作用高于其对映体。水稻和萝卜幼苗的生长受到（-）-IPA的强烈抑制，而（+）-IPA则没有。

项目成果

Yoshida,Noriyuki Sassa,Takeshi: "Synthesis of Acremoauxin A,a New Plant Growth Regulator Produced by Acremonium roseum I 4267" Agric.Biol.Chem.54. 2681-2683 (1990)

Yoshida、Noriyuki Sassa、Takeshi：“Acremoauxin A 的合成，一种由 Acremonia Roseum I 4267 生产的新型植物生长调节剂”Agric.Biol.Chem.54。

Noriyuki Yoshida and Takeshi Sassa: "Synthesis of Acremoauxin A,a New Plant Growth Regulator Produced by Acrenonium reseum I 4267" Agric.Biol.Chem.54. 2681-2683 (1990)

Noriyuki Yoshida 和 Takeshi Sassa：“Acremoauxin A 的合成，一种由 Acrenium reseum I 4267 生产的新型植物生长调节剂”Agric.Biol.Chem.54。

Noriyuki Yoshida and Takeshi Sassa: ""Synthesis of Acremoauxin A, a New Plant Growth Regulator Produced by Acremonium roseum I4267"" Agric. Biol. Chem.54(1). 2681-2683 (1990)

Noriyuki Yoshida 和 Takeshi Sassa：“Acremoauxin A 的合成，一种由 Acremonia Roseum I4267 生产的新型植物生长调节剂”Agric。